Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-beyerene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
ent-cassadiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-cassadien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
ent-cassadien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
ent-sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-sandaracopimaradien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
ent-sandaracopimaradien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
?
isokaurene + [reduced NADPH-hemoprotein reductase] + O2
isokauren-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
isopimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
isopimara-8,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxyisopimara-8,15-diene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
isopimara-8,15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxysandaracopimaradiene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
syn-aphidicolene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxy-syn-aphidicolene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
syn-aphidicolene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
syn-labdatriene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
syn-pimara-7,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxy-syn-pimaradiene + 2alpha-hydroxy-syn-pimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
syn-pimara-9(11),15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
low activity
-
-
?
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
3beta-hydroxy-syn-pimaradiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
syn-stemarene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
syn-stemodene + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
additional information
?
-
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
a three step oxidation
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV8
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV8
a three step oxidation
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV7, Q68YV9, Q6GZ42
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV7, Q68YV9, Q6GZ42
a three step oxidation
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
a three step oxidation
-
-
?
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 30% yield of end-product ent-kaurenoate with ent-kaurenol as substrate
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction, substrate is isolated from leaves of Cryptomeria japonica
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
overall reaction
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
part of gibberelin biosynthetic pathway, decreased KO levels increase the sensitivity to KO inhibitors
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 6% yield of end-product ent-kaurenoate with ent-kaurene as substrate
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
?
-
-
-
?
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
?
low activity
-
-
?
additional information
?
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
?
additional information
?
-
use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, and ent-kauran-16beta-ol
-
-
?
additional information
?
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
?
additional information
?
-
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
?
additional information
?
-
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
?
additional information
?
-
Q68YV8
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
?
additional information
?
-
Q68YV7
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
Q68YV9
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
Q6GZ42
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, ent-kauran-16beta-ol, ent-cassadiene, ent-sandaracopimaradiene, syn-aphidicolene, isopimara-7,15-diene, ent-beyerene, syn-pimara-7,15-diene, syn-stemarene, syn-stemodene, syn-labdatriene, syn-pimara-9(11),15-diene, isopimara-8,15-diene, sandaracopimaradiene
-
-
?
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
?
additional information
?
-
-
enzyme catalyzes the oxidation of ent-kaurene to entkaurenoic acid through three successive monooxygenations. Te rate-limiting step of this oxidation might be the initial step that forms ent-kaurenol
-
-
?
additional information
?
-
enzyme catalyzes the oxidation of ent-kaurene to entkaurenoic acid through three successive monooxygenations. Te rate-limiting step of this oxidation might be the initial step that forms ent-kaurenol
-
-
?
additional information
?
-
-
GC-MS or GLC product identification
-
-
?
additional information
?
-
GC-MS or GLC product identification
-
-
?
additional information
?
-
-
the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
-
-
?
additional information
?
-
-
CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid
-
-
?
additional information
?
-
-
CYP701A26 exhibits substrate promiscuity to catalyze oxidation of other labdane related diterpenes
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
additional information
?
-
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV8
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV7, Q68YV9, Q6GZ42
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
overall reaction
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
part of gibberelin biosynthetic pathway, decreased KO levels increase the sensitivity to KO inhibitors
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
?
additional information
?
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
?
additional information
?
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
?
additional information
?
-
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
?
additional information
?
-
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
?
additional information
?
-
Q68YV8
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
?
additional information
?
-
Q68YV7
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
Q68YV9
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
Q6GZ42
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
?
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
?
additional information
?
-
-
the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
-
-
?
additional information
?
-
-
CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Sherwin, P.F.; Coates, R.M.
Stereospecificity of the oxidation of ent-kauren-19-ol to ent-kaurenal by a microsomal enzyme preparation from Marah macrocarpus
J. Chem. Soc.
1982
1013-1014
1982
Marah macrocarpa
-
brenda
Ashman, P.J.; Mackenzie, A.; Bramley, P.M.
Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi
Biochim. Biophys. Acta
1036
151-157
1990
Cucurbita maxima, Fusarium fujikuroi, Fusarium fujikuroi SG78, Malus sp.
brenda
Archer, C.; Ashman, P.J.; Hedden, P.; Bowyer, J.R.; Bramley, P.M.
Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima
Biochem. Soc. Trans.
20
218S
1992
Cucurbita maxima, Fusarium fujikuroi
brenda
Helliwell, C.A.; Poole, A.; Peacock, W.J.; Dennis, E.S.
Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis
Plant Physiol.
119
507-510
1999
Arabidopsis thaliana
brenda
Helliwell, C.A.; Sullivan, J.A.; Mould, R.M.; Gray, J.C.; Peacock, W.J.; Dennis, E.S.
A plastid envelope location of Arabidopsis ent-kaurene oxidase links the plastid and endoplasmic reticulum steps of the gibberellin biosynthesis pathway
Plant J.
28
201-208
2001
Arabidopsis thaliana (Q93ZB2), Arabidopsis thaliana
brenda
Tudzynski, B.; Hedden, P.; Carrera, E.; Gaskin, P.
The P450-4 gene of Gibberella fujikuroi encodes ent-kaurene oxidase in the gibberellin biosynthesis pathway
Appl. Environ. Microbiol.
67
3514-3522
2001
Fusarium fujikuroi, Fusarium fujikuroi (O94142)
brenda
Swain, S.M.; Singh, D.P.; Helliwell, C.A.; Poole, A.T.
Plants with increased expression of ent-kaurene oxidase are resistant to chemical inhibitors of this gibberellin biosynthesis enzyme
Plant Cell Physiol.
46
284-291
2005
Arabidopsis thaliana
brenda
Davidson, S.E.; Smith, J.J.; Helliwell, C.A.; Poole, A.T.; Reid, J.B.
The pea gene LH encodes ent-kaurene oxidase
Plant Physiol.
134
1123-1134
2004
Pisum sativum (Q6XAF4), Pisum sativum
brenda
Sakamoto, T.; Miura, K.; Itoh, H.; Tatsumi, T.; Ueguchi-Tanaka, M.; Ishiyama, K.; Kobayashi, M.; Agrawal, G.K.; Takeda, S.; Abe, K.; Miyao, A.; Hirochika, H.; Kitano, H.; Ashikari, M.; Matsuoka, M.
An overview of gibberellin metabolism enzyme genes and their related mutants in rice
Plant Physiol.
134
1642-1653
2004
Oryza sativa
brenda
Malonek, S.; Rojas, M.C.; Hedden, P.; Gaskin, P.; Hopkins, P.; Tudzynski, B.
Functional characterization of two cytochrome P450 monooxygenase genes, P450-1 and P450-4, of the gibberellic acid gene cluster in Fusarium proliferatum (Gibberella fujikuroi MP-D)
Appl. Environ. Microbiol.
71
1462-1472
2005
Fusarium proliferatum (Q701P2), Fusarium proliferatum
brenda
Humphrey, T.V.; Richman, A.S.; Menassa, R.; Brandle, J.E.
Spatial organisation of four enzymes from Stevia rebaudiana that are involved in steviol glycoside synthesis
Plant Mol. Biol.
61
47-62
2006
Stevia rebaudiana
brenda
Zhu, S.; Gao, F.; Cao, X.; Chen, M.; Ye, G.; Wei, C.; Li, Y.
The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms
Plant Physiol.
139
1935-1945
2005
Oryza sativa Japonica Group (Q68YV7), Oryza sativa Japonica Group (Q68YV9), Oryza sativa Japonica Group (Q6GZ42), Oryza sativa (Q68YV8)
brenda
Sawada, Y.; Katsumata, T.; Kitamura, J.; Kawaide, H.; Nakajima, M.; Asami, T.; Nakaminami, K.; Kurahashi, T.; Mitsuhashi, W.; Inoue, Y.; Toyomasu, T.
Germination of photoblastic lettuce seeds is regulated via the control of endogenous physiologically active gibberellin content, rather than of gibberellin responsiveness
J. Exp. Bot.
59
3383-3393
2008
Lactuca sativa (B5MEX5), Lactuca sativa (B5MEX6)
brenda
Ko, K.W.; Lin, F.; Katsumata, T.; Sugai, Y.; Miyazaki, S.; Kawaide, H.; Okada, K.; Nojiri, H.; Yamane, H.
Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system
Biosci. Biotechnol. Biochem.
72
3285-3288
2008
Oryza sativa
brenda
Morrone, D.; Chen, X.; Coates, R.M.; Peters, R.J.
Characterization of the kaurene oxidase CYP701A3, a multifunctional cytochrome P450 from gibberellin biosynthesis
Biochem. J.
431
337-344
2010
Arabidopsis thaliana (Q93ZB2), Arabidopsis thaliana, Arabidopsis thaliana Columbia (Q93ZB2)
brenda
Okazaki, M.; Nimitkeatkai, H.; Muramatsu, T.; Aoyama, H.; Ueno, K.; Mizutani, M.; Hirai, N.; Kondo, S.; Ohnishi, T.; Todoroki, Y.
Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8-hydroxylase CYP707A
Bioorg. Med. Chem.
19
406-413
2011
Oryza sativa, Oryza sativa Nipponbare
brenda
Lei, L.; Fan, Z.; Tang, L.; Chen, F.
Molecular cloning and identification of tissue-specific expression of ent-kaurene oxidase gene in Momordica charantia
Afr. J. Biotechnol.
10
15724-15730
2011
Momordica charantia (D5LXQ4)
-
brenda
Miyazaki, S.; Katsumata, T.; Natsume, M.; Kawaide, H.
The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P
FEBS Lett.
585
1879-1883
2011
Physcomitrium patens (F2ZAE0), Physcomitrium patens
brenda
Hamberger, B.; Ohnishi, T.; Hamberger, B.; Seguin, A.; Bohlmann, J.
Evolution of diterpene metabolism: Sitka spruce CYP720B4 catalyzes multiple oxidations in resin acid biosynthesis of conifer defense against insects
Plant Physiol.
157
1677-1695
2011
Picea sitchensis
brenda
Wang, Q.; Hillwig, M.L.; Wu, Y.; Peters, R.J.
CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism
Plant Physiol.
158
1418-1425
2012
Oryza sativa
brenda
Villa-Ruano, N.; Lozoya-Gloria, E.; Betancourt-Jimenez, M.; Herlt, T.; Pacheco-Hernandez, Y.; Castro-Juarez, C.
Functional characterization of ent-kaurene oxidase, MtKO, from Montanoa tomentosa (zoapatle)
Arch. Biol. Sci.
67
193-199
2015
Montanoa tomentosa
-
brenda
Todoroki, Y.; Naiki, K.; Muramatsu, T.; Ohnishi, T.; Ueno, K.; Mizutani, M.; Hirai, N.
A conformationally restricted uniconazole analogue as a specific inhibitor of rice ent-kaurene oxidase, CYP701A6
Bioorg. Med. Chem. Lett.
22
3240-3243
2012
Oryza sativa Japonica Group (Q5Z5R4)
brenda
Mao, H.; Shen, Q.; Wang, Q.
CYP701A26 is characterized as an ent-kaurene oxidase with putative involvement in maize gibberellin biosynthesis
Biotechnol. Lett.
39
1709-1716
2017
Zea mays
brenda
Su, P.; Guan, H.; Zhang, Y.; Wang, X.; Gao, L.; Zhao, Y.; Hu, T.; Zhou, J.; Ma, B.; Tu, L.; Tong, Y.; Huang, L.; Gao, W.
Probing the single key amino acid responsible for the novel catalytic function of ent-kaurene oxidase supported by NADPH-cytochrome P450 reductases in Tripterygium wilfordii
Front. Plant Sci.
8
1756
2017
Tripterygium wilfordii
brenda
Noguchi, C.; Miyazaki, S.; Kawaide, H.; Gotoh, O.; Yoshida, Y.; Aoyama, Y.
Characterization of moss ent-kaurene oxidase (CYP701B1) using a highly purified preparation
J. Biochem.
163
69-76
2018
Physcomitrium patens, Physcomitrium patens (F2ZAE0)
brenda
Yamamura, Y.; Taguchi, Y.; Ichitani, K.; Umebara, I.; Ohshita, A.; Kurosaki, F.; Lee, J.B.
Characterization of ent-kaurene synthase and kaurene oxidase involved in gibberellin biosynthesis from Scoparia dulcis
J. Nat. Med.
72
456-463
2018
Scoparia dulcis (A0A0N7H9X2), Scoparia dulcis (A0A0N9YKX0), Scoparia dulcis
brenda
Itoh, H.; Tatsumi, T.; Sakamoto, T.; Otomo, K.; Toyomasu, T.; Kitano, H.; Ashikari, M.; Ichihara, S.; Matsuoka, M.
A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase
Plant Mol. Biol.
54
533-547
2004
Oryza sativa Japonica Group (Q5Z5R4)
brenda
Mafu, S.; Jia, M.; Zi, J.; Morrone, D.; Wu, Y.; Xu, M.; Hillwig, M.L.; Peters, R.J.
Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis
Proc. Natl. Acad. Sci. USA
113
2526-2531
2016
Oryza sativa Japonica Group (Q5Z5R4), Arabidopsis thaliana (Q93ZB2)
brenda
Su, P.; Tong, Y.; Cheng, Q.; Hu, Y.; Zhang, M.; Yang, J.; Teng, Z.; Gao, W.; Huang, L.
Functional characterization of ent-copalyl diphosphate synthase, kaurene synthase and kaurene oxidase in the Salvia miltiorrhiza gibberellin biosynthetic pathway
Sci. Rep.
6
23057
2016
Salvia miltiorrhiza (A0A0G2RKY1), Salvia miltiorrhiza
brenda