The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.
The enzyme appears in viruses and cellular organisms
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SYSTEMATIC NAME
IUBMB Comments
4-isopropylbenzoate:oxygen 2,3-oxidoreductase
The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.
Substrates: - Products: the substrate for the ring cleavage of 2,3-dihydroxy-p-cumate is formed from p-cumate in two reactions via a dihydrodiol intermediate (2,3-dihydroxy-4-isopropylcyclohexa-4,6-dienoate). Both hydroxyl atoms of the dihydrodiol are derived from the same molecule of 02
Substrates: - Products: the substrate for the ring cleavage of 2,3-dihydroxy-p-cumate is formed from p-cumate in two reactions via a dihydrodiol intermediate (2,3-dihydroxy-4-isopropylcyclohexa-4,6-dienoate). Both hydroxyl atoms of the dihydrodiol are derived from the same molecule of 02
a mutant of Pseudomonas putida, which is unable to grow with p-cumate, accumulates 2,3-dihydroxy-4-isoproplycyclohexa-4,6-dienoate. Both hydroxyl atoms of the dihydrodiol are derived from the same molecule of 02
componentns of p-cumate 2,3-dioxygenase are subunit CmtAa to CmtAd, i.e. ferredoxin reductase, large subunit of the terminal dioxygenase, small subunit of the terminal dioxygenase, and ferredoxin, respectively
componentns of p-cumate 2,3-dioxygenase are subunit CmtAa to CmtAd, i.e. ferredoxin reductase, large subunit of the terminal dioxygenase, small subunit of the terminal dioxygenase, and ferredoxin, respectively
componentns of p-cumate 2,3-dioxygenase are subunit CmtAa to CmtAd, i.e. ferredoxin reductase, large subunit of the terminal dioxygenase, small subunit of the terminal dioxygenase, and ferredoxin, respectively
enrichment of mutants defective in the utilization of p-cymene as sole carbon source by incubation of cells in minimal growth media containing p-cymene, p-chlorotoluene, p-bromotoluene, alpha-chloro-p-xylene, or p-iodobenzoate. Several classes of mutants can be isolated, p-cymene hydroxylase and p-cumate 2,3-dioxygenase are likely to be multicomponent enzymes
Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: Chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon
J. Bacteriol.
178
1351-1362
1996
Pseudomonas putida (Q51973 and Q51974 and Q51975 and Q51978), Pseudomonas putida, Pseudomonas putida DSM 6899 (Q51973 and Q51974 and Q51975 and Q51978)
Agullo, L.; Romero-Silva, M.J.; Domenech, M.; Seeger, M.
p-Cymene promotes its catabolism through the p-cymene and the p-cumate pathways, activates a stress response and reduces the biofilm formation in Burkholderia xenovorans LB400
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