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5'-AUUCUCAm6AC-3' + 2-oxoglutarate + O2
5'-AUUCUCAAC-3' + formaldehyde + succinate + CO2
-
ssRNA substrate
-
-
?
5'-CUCGAUACG(m6A)UCCGGUCAAA-3' + 2-oxoglutarate + O2
5'-CUCGAUACGAUCCGGUCAAA-3' + formaldehyde + succinate + CO2
-
-
-
?
5'-CUGGm6ACUGG-3' + 2-oxoglutarate + O2
5'-CUGGACUGG-3' + formaldehyde + succinate + CO2
-
ssRNA substrate
-
-
?
5'-m6ACUGACUAG-3' + 2-oxoglutarate + O2
5'-m6ACUGACUAG-3' + formaldehyde + succinate + CO2
-
ssRNA substrate
-
-
?
5'-UACACUCGAUCUGG(m6A)CUAAAGCUGCUC-3'-biotin + 2-oxoglutarate + O2
5'-UACACUCGAUCUGGCUAAAGCUGCUC-3'-biotin + formaldehyde + succinate + CO2
-
-
-
-
?
biotin-AAGCTCCCATGTTAGGm6ATCAGTGTCTCGAG-biotin + 2-oxoglutarate + O2
biotin-AAGCTCCCATGTTAGGATCAGTGTCTCGAG-biotin + formaldehyde + succinate + CO2
-
-
-
?
CATGTTAGGATCAGTG + 2-oxoglutarate + O2
CATGTTAGGm6ATCAGTG + formaldehyde + succinate + CO2
-
-
-
?
CCCC(m6A)CCCCCCCCC + 2-oxoglutarate + O2
? + formaldehyde + succinate + CO2
-
30% demethylation
-
-
?
GA(m6A)CA + 2-oxoglutarate + O2
GAACA + formaldehyde + succinate + CO2
-
39% demethylation
-
-
?
GCGG(m6A)CUCCAGAUG + 2-oxoglutarate + O2
GCGGACUCCAGAUG + formaldehyde + succinate + CO2
-
31% demethylation
-
-
?
GG(m6A)CU + 2-oxoglutarate + O2
GGACU + formaldehyde + succinate + CO2
-
37% demethylation
-
-
?
N3-methylcytosine in single-stranded DNA + 2-oxoglutarate + O2
cytosine in single-stranded DNA + formaldehyde + succinate + CO2
N3-methylthymine in single-stranded DNA + 2-oxoglutarate + O2
thymine in single-stranded DNA + formaldehyde + succinate + CO2
N3-methyluracil in single-stranded mRNA + 2-oxoglutarate + O2
uracil in single-stranded mRNA + formaldehyde + succinate + CO2
N6,N6-dimethyladenosine + 2 2-oxoglutarate + 2 O2
adenosine + 2 formaldehyde + 2 succinate + 2 CO2
-
-
-
-
?
N6-methyladenine + 2-oxoglutarate + O2
adenine + formaldehyde + succinate + CO2
-
-
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
N6-methyladenine in NANOG mRNA + 2-oxoglutarate + O2
adenine in NANOG mRNA + formaldehyde + succinate + CO2
-
-
-
-
?
N6-methyladenine in NEAT1 + 2-oxoglutarate + O2
adenine in NEAT1 + formaldehyde + succinate + CO2
substrate i.e. nuclear paraspeckle assembly transcript 1
-
-
?
N6-methyladenine in RNA + 2-oxoglutarate + O2
adenine in RNA + formaldehyde + succinate + CO2
-
-
-
-
?
N6-methyladenine in single-stranded DNA + 2-oxoglutarate + O2
adenine in single-stranded DNA + formaldehyde + succinate + CO2
the ALKBH5 catalytic domain (residues 74294) is active and can demethylate ssDNA and ssRNA with similar activity. m6A ssDNA may not be a physiologically relevant ALKBH5 substrate
-
-
?
N6-methyladenine in single-stranded DNA oligonucleotide + 2-oxoglutarate + O2
adenine in single-stranded DNA oligonucleotide + formaldehyde + succinate + CO2
-
-
-
?
N6-methyladenine in ZNF333 mRNA + 2-oxoglutarate + O2
adenine in ZNF333 mRNA + formaldehyde + succinate + CO2
-
-
-
?
N6-methyladenosine + 2-oxoglutarate + O2
adenosine + formaldehyde + succinate + CO2
-
-
-
-
?
additional information
?
-
N3-methylcytosine in single-stranded DNA + 2-oxoglutarate + O2
cytosine in single-stranded DNA + formaldehyde + succinate + CO2
strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA
-
-
?
N3-methylcytosine in single-stranded DNA + 2-oxoglutarate + O2
cytosine in single-stranded DNA + formaldehyde + succinate + CO2
strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA
-
-
?
N3-methylthymine in single-stranded DNA + 2-oxoglutarate + O2
thymine in single-stranded DNA + formaldehyde + succinate + CO2
strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA. Negligible activities against N3-methylthymine in double-stranded DNA
-
-
?
N3-methylthymine in single-stranded DNA + 2-oxoglutarate + O2
thymine in single-stranded DNA + formaldehyde + succinate + CO2
strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA. Negligible activities against N3-methylthymine in double-stranded DNA
-
-
?
N3-methyluracil in single-stranded mRNA + 2-oxoglutarate + O2
uracil in single-stranded mRNA + formaldehyde + succinate + CO2
2-fold preference for N3-methyluracil in single-stranded mRNA as the substrate over N3-methylthymine in single-stranded DNA. Slightly higher efficiency over that of N3-methylthymine in single-stranded DNA
-
-
?
N3-methyluracil in single-stranded mRNA + 2-oxoglutarate + O2
uracil in single-stranded mRNA + formaldehyde + succinate + CO2
2-fold preference for N3-methyluracil in single-stranded mRNA as the substrate over N3-methylthymine in single-stranded DNA. Slightly higher efficiency over that of N3-methylthymine in single-stranded DNA
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
-
737368, 737446, 738271, 738709, 738753, 739688, 742218, 742336, 743216, 743373, 743572, 743808 -
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
-
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
m6A in nuclear RNA is the physiological substrate of the enzyme
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
m6A modification preferentially appears after G in the conserved motif RRm6ACH (R is A/G and H is A/C/U)
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
15-mer m6A-containing ssRNA or 15-mer m3U-containing ssRNA. Over 50-fold preference of the enzyme for m6A (at pH 7.0) over m3U (qat pH 6.0)
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
the ALKBH5 catalytic domain (residues 74294) is active and can demethylate ssDNA and ssRNA with similar activity
-
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
-
-
?
additional information
?
-
very low activity toward repairing N1-methyladenine in 49 mer single-stranded DNA
-
-
?
additional information
?
-
negligible activity with m6A-containing dsDNA and dsRNA. Treatment with one molar equivalent of enzyme at 16°C overnight results in a demethylation yield of 40% for dsDNA and 24% for dsRNA, respectively
-
-
?
additional information
?
-
-
negligible activity with m6A-containing dsDNA and dsRNA. Treatment with one molar equivalent of enzyme at 16°C overnight results in a demethylation yield of 40% for dsDNA and 24% for dsRNA, respectively
-
-
?
additional information
?
-
-
the enzyme binds preferentially to pre-mRNAs in intronic regions, in the proximity of alternatively spliced exons and poly(A) sites
-
-
?
additional information
?
-
very low activity toward repairing N1-methyladenine in 49 mer single-stranded DNA
-
-
?
additional information
?
-
-
poor substrates: N1-methyladenosine, O2'-methyladenosine, 5-methylcytosine, and 5-methyluridine
-
-
-
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additional information
isoforms ALKBH9A and ALKBH9C are not involved in alfalfa mosaic virus infrection
malfunction
Alkbh5-deficient male mice have increased m6A in mRNA and are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase stage spermatocytes
malfunction
Alkbh5-deficient male mice have increased m6A in mRNA and are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase stage spermatocytes
malfunction
-
enzyme knockdown in MDAMB-231 human breast cancer cells significantly reduces their capacity for tumor initiation as a result of reduced numbers of breast cancer stem cells
metabolism
N6-methylation of adenosine is the most ubiquitous and abundant modification of nucleoside in eukaryotic mRNA and long non-coding RNA. This modification plays an essential role in the regulation of mRNA translation and RNA metabolism
metabolism
-
the enzyme enhances NANOG mRNA stability by catalyzing m6A demethylation
metabolism
comparison of isoforms FTO and ALKBH5. FTO behaves like a classical nonheme Fe(II)-2OG-dependent dioxygenase by performing stepwise oxidation, whereas ALKBH5 catalyzes a unique direct 6-methyladenosine-to-adenosine conversion with rapid release of formaldehyde. A catalytic R130/K132/Y139 triad within ALKBH5 facilitates release of formaldehyde via an covalent-based demethylation mechanism with direct detection of a covalent intermediate. In a mechanistic model for ALKBH5, K132 promotes Schiff base formation on hm6A, which may then undergo subsequent nucleophilic attack by K132 or Y139. Y139 may alternatively play a role in nucleobase recognition via hydrogen bonding to the N6 nitrogen. Formation of a methylene bridge between K132 and Y139 is a probable intermediate prior to hydrolysis and may facilitate release of adeosine
metabolism
comparison of isoforms FTO and ALKBH5. FTO follows a traditional oxidative N-demethylation pathway to catalyze conversion of m6A to hm6A with subsequent slow release of adenosine and formaldehyde. FTO behaves like a classical nonheme Fe(II)-2OG-dependent dioxygenase by performing stepwise oxidation, whereas ALKBH5 catalyzes a unique direct 6-methyladenosine-to-adeosine conversion. FTO gives 6-hydroxymethyladenosine as a major product and 6-formyladenosine as a minorproduct
metabolism
-
during the reaction, the hv-excited FMN abstracts one electron and one proton from N6-methyl in m6A, probably via a proton-coupled electron transfer pathway, to give the deprotonated amine radical cation and the semiquinone radical of FMNH. A second proton-coupled electron transfer then leads to the formation of the corresponding imine with the concomitant reduction of the FMNH radical to FMNH2. The N,O-hemiacetal hm6A is formed from the hydration of II[4a,b] and it quickly oxidizes to N6-formyladenosine, followed by water-assisted decomposition to yield the adenosine and formic acid. Alternatively, a direct loss of formaldehyde from N6-formyladenosine would also afford adenosine
physiological function
plays a role in spermatogenesis
physiological function
-
enzyme expression promotes m6A RNA demethylation in hypoxic breast cancer cells
physiological function
-
the enzyme FTO plays a role in human obesity and energy utilization. FTO is involved in various diseases including cardiovascular diseases,Alzheimer's disease, type II diabetes, breast cancer, and end-stage renal disease. The enzyme ALKHB5 is involoved in sperm development
physiological function
-
the enzyme triggers inclusion of alternatively spliced exons and regulates expression of last exons
physiological function
ALKBH10B mutants are hypersensitive to abscisic acid, osmotic and salt stress during seed germination. The expression of several abscisic acid response genes is upregulated in the mutants. abscisic acid signaling genes, including PYR1, PYL7, PYL9, ABI1, and SnRK2.2 are N6-methyladenine-hypermethylated in the mutants after abscisic acid treatment
physiological function
ALKBH5 demethylates zinc finger protein ZNF333 mRNA, leading to enhanced ZNF333 expression by abolishing m6A-YTHDF2-dependent mRNA degradation. ALKBH5 activates CDX2 and downstream intestinal markers by targeting the ZNF333/CYLD axis and activating NF-kappaB signaling. p65, the key transcription factor of the canonical NF-kappaB pathway, enhances the transcription activity of ALKBH5 in the nucleus. ALKBH5 levels are positively correlated with ZNF333 and CDX2 levels in gastric intestinal metaplasia tissues
physiological function
ALKBH5 mRNA and protein expression are upregulated during osteoblast differentiation. ALKBH5 knockdown suppresses osteoblast differentiation, mineralization, and the expression of osteogenic biomarkers. ALKBH5 overexpression promotes osteogenesis. Knockdown of ALKBH5 significantly impaires the mRNA stability of the transcription factor Runx2
physiological function
ALKBH9B interacts with the coat protein of alfalfa mosaic virus, causing a profound impact on the viral infection cycle. Residues located between 387 and 427 are critical for the interaction with the alfalfa mosaic virus coat protein, which should be critical for modulating the viral infection process. ALKBH9B deletions of either N-terminal 20 residues or the C-terminal's last 40 amino acids impede their accumulation in siRNA bodies
physiological function
ALKBH9B might be required by alfalfa mosaic virus to invade the vascular tissues. Alfalfa mosaic virus cell-to-cell movement among mesophyll cells is reduced in the absence of ALKBH9B and absence of ALKBH9B affects the viral replication cycle
physiological function
-
flavin mononucleotide functions as an artificial m6A demethylase. FMN mediates substantial photochemical demethylation of m6A residues of RNA in live cells
physiological function
FTO acts as a senescence-retarding protein via N6-methyladenosine, and transcription factor subunit FOS knockdown significantly alleviates the aging of FTO-knockdown granulosa cells. FTO-knockdown granulosa cells show faster aging-related phenotypes, and increased N6-methyladenosine levels are found in the FOS-mRNA 3'UTR
physiological function
FTO loss-of-function mutations reduce the proliferation rate of cancer cells. FTO knockdown also inhibits the colony formation ability of lung cancer cells. FTO knockdown reduces lung cancer cells growth in vivo. FTO decreases the m6A level and increases mRNA stability of ubiquitin-specific protease (USP7), which relies on the demethylase activity of FTO
physiological function
HCT-116 cells show high expression of both FTO and programmed cell death-ligand 1 (PD-L1) proteins. The knockdown of FTO decreases mRNA and protein levels of PD-L1 in HCT-116 cells. Upon depletion of FTO in HCT-116 cells in the presence of IFN-gamma to upregulate PD-L1 expression, PD-L1 expression is reduced in an IFN-gamma signaling-independent manner. PD-L1 mRNA is m6A-modified and FTO binds to the PD-L1 mRNA in HCT-116 cells
physiological function
knockdown of FTO increases m6A methylation in critical protumorigenic melanoma cell-intrinsic genes including PD-1, CXCR4, and SOX10, leading to increased RNA decay through the m6A reader YTHDF2. Knockdown of FTO sensitizes melanoma cells to interferon gamma
physiological function
knockdown of FTO increases m6A methylation in critical protumorigenic melanoma cell-intrinsic genes including PD-1, CXCR4, and SOX10, leading to increased RNA decay through the m6A reader YTHDF2. Knockdown of FTO sensitizes melanoma cells to interferon gamma and sensitizes melanoma to anti-PD-1 treatment in mice
physiological function
ROS significantly induces global mRNA N6-methyladenosine levels by modulating ALKBH5 post-translational modifications, leading to the rapid and efficient induction of thousands of genes. DNA damage repair genes are ALKBH5 downstream targets induced by ROS. ROS promotes ALKBH5 SUMOylation through activating ERK/JNK signaling, leading to inhibition of ALKBH5m6A demethylase activity by blocking substrate accessibility
physiological function
substrate NEAT1 is a potential binding long noncoding lncRNA of ALKBH5. NEAT1 is overexpressed in gastric cancer cells and tissue. Knockdown of NEAT1 significantly represses invasion and metastasis of gastric cancer cells. ALKBH5 affects the m6A level of NEAT1. The binding of ALKBH5 and NEAT1 influences the expression of EZH2 (a subunit of the polycomb repressive complex) and thus affects gastric cancer invasion and metastasis
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Jia, G.; Yang, C.G.; Yang, S.; Jian, X.; Yi, C.; Zhou, Z.; He, C.
Oxidative demethylation of 3-methylthymine and 3-methyluracil in single-stranded DNA and RNA by mouse and human FTO
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2008
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42
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Homo sapiens (Q6P6C2), Homo sapiens
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69
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17
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588
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Danio rerio (Q08BA6), Danio rerio
brenda
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Sequencing of FTO and ALKBH5 in men undergoing infertility work-up identifies an infertility-associated variant and two missense mutations
Fertil. Steril.
105
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Homo sapiens
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289
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Homo sapiens (Q6P6C2), Homo sapiens
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289
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A Radioactivity-based assay for screening human m6A-RNA methyltransferase, METTL3-METTL14 complex, and demethylase ALKBH5
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21
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49
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brenda
Jia, G.; Fu, Y.; Zhao, X.; Dai, Q.; Zheng, G.; Yang, Y.; Yi, C.; Lindahl, T.; Pan, T.; Yang, Y.G.; He, C.
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7
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Homo sapiens (Q9C0B1), Homo sapiens
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Crystal structure of the FTO protein reveals basis for its substrate specificity
Nature
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Homo sapiens (Q9C0B1)
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Huang, Y.; Yan, J.; Li, Q.; Li, J.; Gong, S.; Zhou, H.; Gan, J.; Jiang, H.; Jia, G.F.; Luo, C.; Yang, C.G.
Meclofenamic acid selectively inhibits FTO demethylation of m6A over ALKBH5
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43
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Homo sapiens (Q6P6C2), Homo sapiens
brenda
Zhang, C.; Samanta, D.; Lu, H.; Bullen, J.W.; Zhang, H.; Chen, I.; He, X.; Semenza, G.L.
Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA
Proc. Natl. Acad. Sci. USA
113
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Homo sapiens
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Zou, S.; Toh, J.D.; Wong, K.H.; Gao, Y.G.; Hong, W.; Woon, E.C.
N6-methyladenosine: a conformational marker that regulates the substrate specificity of human demethylases FTO and ALKBH5
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6
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Homo sapiens
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Chandola, U.; Das, R.; Panda, B.
Role of the N6-methyladenosine RNA mark in gene regulation and its implications on development and disease
Brief. Funct. Genomics
14
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2015
Homo sapiens
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Liu, K.; Ding, Y.; Ye, W.; Liu, Y.; Yang, J.; Liu, J.; Qi, C.
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Curr. Protein Pept. Sci.
17
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Homo sapiens
brenda
Ye, F.; Zhang, L.; Jin, L.; Zheng, M.; Jiang, H.; Luo, C.
Repair of methyl lesions in RNA by oxidative demethylation
MedChemComm
5
1797-1803
2014
Homo sapiens
-
brenda
Bartosovic, M.; Molares, H.C.; Gregorova, P.; Hrossova, D.; Kudla, G.; Vanacova, S.
N6-methyladenosine demethylase FTO targets pre-mRNAs and regulates alternative splicing and 3-end processing
Nucleic Acids Res.
45
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2017
Homo sapiens
brenda
Berulava, T.; Rahmann, S.; Rademacher, K.; Klein-Hitpass, L.; Horsthemke, B.
N6-adenosine methylation in miRNAs
PLoS ONE
10
e0118438
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Homo sapiens
brenda
Yang, Y.; Huang, W.; Huang, J.T.; Shen, F.; Xiong, J.; Yuan, E.F.; Qin, S.S.; Zhang, M.; Feng, Y.Q.; Yuan, B.F.; Liu, S.M.
Increased N6-methyladenosine in human sperm RNA as a risk factor for asthenozoospermia
Sci. Rep.
6
24345
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Homo sapiens
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Selberg, S.; Seli, N.; Kankuri, E.; Karelson, M.
Rational design of novel anticancer small-molecule RNA m6A demethylase ALKBH5 inhibitors
ACS Omega
6
13310-13320
2021
Homo sapiens (Q6P6C2)
brenda
Zhang, Y.; Li, Q.N.; Zhou, K.; Xu, Q.; Zhang, C.Y.
Identification of specific N6-methyladenosine RNA demethylase FTO inhibitors by single-quantum-dot-based FRET nanosensors
Anal. Chem.
92
13936-13944
2020
Homo sapiens (Q6P6C2), Homo sapiens (Q9C0B1), Homo sapiens
brenda
Xie, L.J.; Yang, X.T.; Wang, R.L.; Cheng, H.P.; Li, Z.Y.; Liu, L.; Mao, L.; Wang, M.; Cheng, L.
Identification of flavin mononucleotide as a cell-active artificial N6-methyladenosine RNA demethylase
Angew. Chem. Int. Ed. Engl.
58
5028-5032
2019
synthetic construct
brenda
Li, J.; Han, Y.; Zhang, H.; Qian, Z.; Jia, W.; Gao, Y.; Zheng, H.; Li, B.
The m6A demethylase FTO promotes the growth of lung cancer cells by regulating the m6A level of USP7 mRNA
Biochem. Biophys. Res. Commun.
512
479-485
2019
Homo sapiens (Q9C0B1)
brenda
Tsuruta, N.; Tsuchihashi, K.; Ohmura, H.; Yamaguchi, K.; Ito, M.; Ariyama, H.; Kusaba, H.; Akashi, K.; Baba, E.
RNA N6-methyladenosine demethylase FTO regulates PD-L1 expression in colon cancer cells
Biochem. Biophys. Res. Commun.
530
235-239
2020
Homo sapiens (Q9C0B1)
brenda
Du, T.; Li, G.; Yang, J.; Ma, K.
RNA demethylase Alkbh5 is widely expressed in neurons and decreased during brain development
Brain Res. Bull.
163
150-159
2020
Mus musculus (Q3TSG4), Mus musculus
brenda
Jiang, Z.X.; Wang, Y.N.; Li, Z.Y.; Dai, Z.H.; He, Y.; Chu, K.; Gu, J.Y.; Ji, Y.X.; Sun, N.X.; Yang, F.; Li, W.
The m6A mRNA demethylase FTO in granulosa cells retards FOS-dependent ovarian aging
Cell Death Dis.
12
744
2021
Homo sapiens (Q9C0B1), Homo sapiens
brenda
Feng, L.; Fan, Y.; Zhou, J.; Li, S.; Zhang, X.
The RNA demethylase ALKBH5 promotes osteoblast differentiation by modulating Runx2 mRNA stability
FEBS Lett.
595
2007-2014
2021
Rattus norvegicus (D3ZKD3)
brenda
Martinez-Perez, M.; Gomez-Mena, C.; Alvarado-Marchena, L.; Nadi, R.; Micol, J.L.; Pallas, V.; Aparicio, F.
The m6A RNA Demethylase ALKBH9B plays a critical role for vascular movement of alfalfa mosaic virus in Arabidopsis
Front. Microbiol.
12
745576
2021
Arabidopsis thaliana (Q9SL49)
brenda
Alvarado-Marchena, L.; Marquez-Molins, J.; Martinez-Perez, M.; Aparicio, F.; Pallas, V.
Mapping of functional subdomains in the atALKBH9B m6A-demethylase required for its binding to the viral RNA and to the coat protein of alfalfa mosaic virus
Front. Plant Sci.
12
701683
2021
Arabidopsis thaliana (Q9SL49)
brenda
Tang, J.; Yang, J.; Duan, H.; Jia, G.
ALKBH10B, an mRNA m6A demethylase, modulates ABA response during seed germination in Arabidopsis
Front. Plant Sci.
12
712713
2021
Arabidopsis thaliana (Q9ZT92)
brenda
Zhang, J.; Guo, S.; Piao, H.; Wang, Y.; Wu, Y.; Meng, X.; Yang, D.; Zheng, Z.; Zhao, Y.
ALKBH5 promotes invasion and metastasis of gastric cancer by decreasing methylation of the lncRNA NEAT1
J. Physiol. Biochem.
75
379-389
2019
Homo sapiens (Q6P6C2)
brenda
Niu, Y.; Lin, Z.; Wan, A.; Chen, H.; Liang, H.; Sun, L.; Wang, Y.; Li, X.; Xiong, X.F.; Wei, B.; Wu, X.; Wan, G.
RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
Mol. Cancer
18
46
2019
Homo sapiens (Q9C0B1), Homo sapiens
brenda
Yue, B.; Cui, R.; Zheng, R.; Jin, W.; Song, C.; Bao, T.; Wang, M.; Yu, F.; Zhao, E.
Essential role of ALKBH5-mediated RNA demethylation modification in bile acid-induced gastric intestinal metaplasia
Mol. Ther. Nucleic Acids
26
458-472
2021
Homo sapiens (Q6P6C2)
brenda
Yang, S.; Wei, J.; Cui, Y.H.; Park, G.; Shah, P.; Deng, Y.; Aplin, A.E.; Lu, Z.; Hwang, S.; He, C.; He, Y.Y.
m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade
Nat. Commun.
10
2782
2019
Mus musculus (Q8BGW1), Homo sapiens (Q9C0B1), Homo sapiens
brenda
Yu, F.; Wei, J.; Cui, X.; Yu, C.; Ni, W.; Bungert, J.; Wu, L.; He, C.; Qian, Z.
Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response
Nucleic Acids Res.
49
5779-5797
2021
Homo sapiens (Q6P6C2)
brenda
Toh, J.; Crossley, S.; Bruemmer, K.; Ge, E.; He, D.; Iovan, D.; Chang, C.
Distinct RNA N-demethylation pathways catalyzed by nonheme iron ALKBH5 and FTO enzymes enable regulation of formaldehyde release rates
Proc. Natl. Acad. Sci. USA
117
25284-25292
2020
Homo sapiens (Q6P6C2), Homo sapiens (Q9C0B1)
brenda