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4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
6-deoxy-D-glucose + 2,6-dichlorophenol indophenol
?
-
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
6-deoxy-D-glucose + O2
?
-
hexoses as substrates determined
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
arabinoxylan + O2
?
-
-
-
?
beech wood xylan + O2
?
-
-
-
?
beta-(1->3,1->4)-glucan + O2
?
beta-D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + ?
-
DCIP, electron acceptor specificity determined
-
-
?
beta-D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
kinetic parameters for various sugars shown, enzyme properties compared to other sugar oxidizing enzymes
-
-
?
carboxymethyl cellulose + O2
?
cello-oligosaccharide + acceptor
?
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
?
-
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
cellobiose + acceptor
?
-
-
-
?
cellobiose + O2
?
-
disaccharides as substrates determined
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
cellohexaose + O2
?
-
oligosaccharides as substrates determined
-
-
?
cellohexaose + O2
cellohexaono-1,5-lactone + H2O2
cellohexaose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
?
-
oligosaccharides as substrates determined
-
-
?
cellopentaose + O2
cellopentaono-1,5-lactone + H2O2
cellopentaose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
?
-
oligosaccharides as substrates determined
-
-
?
cellotetraose + O2
cellotetraono-1,5-lactone + H2O2
cellotetraose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
?
-
oligosaccharides as substrates determined
-
-
?
cellotriose + O2
? + H2O2
cellotriose + O2
cellotriono-1,5-lactone + H2O2
cellotriose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
D-fructose + O2
?
-
oligosaccharides as substrates determined
-
-
?
D-galactose + 2,6-dichlorophenol indophenol
?
-
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
D-galactose + O2
?
-
hexoses as substrates determined
-
-
?
D-galactose + O2
? + H2O2
-
-
-
?
D-glucose + O2
? + H2O2
highest catalytic efficiency with D-glucose
-
-
?
D-glucose + O2
D-glucono-1,5-lactone + H2O2
D-mannose + O2
?
-
hexoses as substrates determined
-
-
?
D-xylobiose + O2
?
-
oligosaccharides as substrates determined
-
-
?
D-xylose + 2,6-dichlorophenol indophenol
?
-
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
D-xylose + O2
?
-
pentoses as substrates determined
-
-
?
D-xylose + O2
D-xylono-1,5-lactone + H2O2
debranched soluble oat spelt xylan + acceptor
?
-
-
-
?
glucomannan + O2
?
-
-
-
?
L-arabinose + 2,6-dichlorophenol indophenol
?
-
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
L-arabinose + O2
?
-
pentoses as substrates determined
-
-
?
lactose + 2,6-dichlorophenol indophenol
?
-
substrate specificity for alpha-lactose and beta-lactose determined
-
-
?
lactose + acceptor
?
-
-
-
?
lactose + O2
?
-
substrate specificity for alpha-lactose and beta-lactose determined
-
-
?
malto-oligosaccharide + acceptor
?
-
-
-
?
maltohexaose + O2
?
-
oligosaccharides as substrates determined
-
-
?
maltopentaose + O2
?
-
oligosaccharides as substrates determined
-
-
?
maltose + 2,6-dichlorophenol indophenol
?
-
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
maltose + acceptor
?
-
-
-
?
maltose + O2
4-O-alpha-D-glucopyranosyl-D-glucono-1,5-lactone + H2O2
-
-
-
?
maltose + O2
?
-
disaccharides as substrates determined
-
-
?
maltose + O2
maltono-1,5-lactone + H2O2
-
-
-
?
maltotetraose + O2
?
-
oligosaccharides as substrates determined
-
-
?
maltotriose + O2
?
-
oligosaccharides as substrates determined
-
-
?
N-acetyl-D-glucosamine + O2
? + H2O2
-
-
-
?
propoxylated wheat bran hemicellulose + O2
?
-
-
-
?
soluble oat spelt xylan + acceptor
?
-
-
-
?
wheat bran hemicellulose + O2
?
-
-
-
?
xylobiose + 2,6-dichlorophenol indophenol
?
-
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
xylobiose + O2
?
-
disaccharides as substrates determined
-
-
?
xylobiose + O2
? + H2O2
-
-
-
?
xylobiose + O2
xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
xylobiose + O2
xylobiono-1,5-lactone + H2O2
xyloglucan + O2
?
-
-
-
?
xylohexaose + O2
D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
xylohexaose + O2
xylohexaono-1,5-lactone + H2O2
xylooligosaccharide + O2
? + H2O2
-
-
-
?
xylopentaose + O2
D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
xylopentaose + O2
xylopentaono-1,5-lactone + H2O2
xylotetraose + O2
xylotetraono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
? + H2O2
-
-
-
?
xylotriose + O2
xyl-beta-(1->4)-xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
xylotriono-1,5-lactone + H2O2
additional information
?
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
Avicel + O2
?
-
-
-
?
beta-(1->3,1->4)-glucan + O2
?
-
-
-
?
beta-(1->3,1->4)-glucan + O2
?
-
-
-
?
carboxymethyl cellulose + O2
?
-
-
-
?
carboxymethyl cellulose + O2
?
-
-
-
?
cellobiose + O2
? + H2O2
-
-
-
?
cellobiose + O2
? + H2O2
preferred substrate
-
-
?
cellobiose + O2
? + H2O2
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
-
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
cellohexaono-1,5-lactone + H2O2
-
-
-
-
?
cellohexaose + O2
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
cellopentaono-1,5-lactone + H2O2
-
-
-
-
?
cellopentaose + O2
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
cellotetraono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
cellotetraono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
? + H2O2
-
-
-
?
cellotriose + O2
? + H2O2
-
-
-
?
cellotriose + O2
cellotriono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
cellotriono-1,5-lactone + H2O2
-
-
-
-
?
cellotriose + O2
cellotriono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
cellotriono-1,5-lactone + H2O2
-
-
-
?
cellulose + O2
?
-
-
-
?
D-glucose + acceptor
?
-
-
-
-
?
D-glucose + acceptor
?
-
-
-
?
D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
-
-
-
?
D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
? + H2O2
-
-
-
?
D-xylose + O2
? + H2O2
-
-
-
?
D-xylose + O2
D-xylono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
D-xylono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
D-xylono-1,5-lactone + H2O2
-
-
-
-
?
D-xylose + O2
D-xylono-1,5-lactone + H2O2
-
-
-
?
maltose + O2
? + H2O2
-
-
-
?
maltose + O2
? + H2O2
-
-
-
?
xylobiose + O2
xylobiono-1,5-lactone + H2O2
highest activity
-
-
?
xylobiose + O2
xylobiono-1,5-lactone + H2O2
-
highest activity
-
-
?
xylobiose + O2
xylobiono-1,5-lactone + H2O2
highest activity
-
-
?
xylohexaose + O2
xylohexaono-1,5-lactone + H2O2
-
-
-
?
xylohexaose + O2
xylohexaono-1,5-lactone + H2O2
-
-
-
-
?
xylopentaose + O2
xylopentaono-1,5-lactone + H2O2
-
-
-
?
xylopentaose + O2
xylopentaono-1,5-lactone + H2O2
-
-
-
-
?
xylotriose + O2
xylotriono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
xylotriono-1,5-lactone + H2O2
-
-
-
-
?
xylotriose + O2
xylotriono-1,5-lactone + H2O2
-
-
-
?
additional information
?
-
no activity with mannose
-
-
?
additional information
?
-
-
no activity with mannose
-
-
?
additional information
?
-
enzyme GOOX oxidizes the anomeric carbon of carbohydrate substrates through an FAD reduction mechanism. The enzyme displays a comparatively broad substrate profile, ranging from D-xylose, and galactose, to malto-, cello- and xylooligosaccharides. GOOX oxidizes oligosaccharides more effectively than monosaccharides
-
-
?
additional information
?
-
no activity with mannose
-
-
?
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0.3
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
52 - 75
6-deoxy-D-glucose
0.078 - 0.09
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
0.052 - 0.059
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
0.06 - 0.26
cellotetraose
56 - 950
N-acetyl-D-glucosamine
0.067 - 0.11
xylotetraose
52
6-deoxy-D-glucose
-
O2 used as electron acceptor
75
6-deoxy-D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.078
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.09
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.052
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.059
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.039
cellobiose
mutant enzyme A38V, at pH 8.0 and 37°C
0.04
cellobiose
wild type enzyme, at pH 8.0 and 37°C
0.05
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.061
cellobiose
wild type protein
0.07
cellobiose
wild type enzyme, at 37°C and pH 8.0
0.08
cellobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.083
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
0.13
cellobiose
-
O2 used as electron acceptor
0.22
cellobiose
H70A mutant protein
0.25
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
0.25
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
0.25
cellobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.25
cellobiose
pH 8.0, 37°C, recombinant mutant Y300A
0.38
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
0.4
cellobiose
C130A mutant protein
0.06
cellohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.08
cellohexaose
wild type enzyme, at pH 8.0 and 37°C
0.08
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.1
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.16
cellohexaose
-
O2 used as electron acceptor
0.31
cellohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.03
cellopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.057
cellopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
cellopentaose
wild type enzyme, at pH 8.0 and 37°C
0.09
cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.15
cellopentaose
-
O2 used as electron acceptor
0.3
cellopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.06
cellotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.06
cellotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
0.07
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
0.09
cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.26
cellotetraose
-
O2 used as electron acceptor
0.05
cellotriose
wild type enzyme, at pH 8.0 and 37°C
0.05
cellotriose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
cellotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.08
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.085
cellotriose
wild type enzyme, at 37°C and pH 8.0
0.11
cellotriose
-
O2 used as electron acceptor
0.11
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
0.21
cellotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.25
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
0.25
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
0.25
cellotriose
pH 8.0, 37°C, recombinant mutant Y300A
0.44
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
36.1
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
61
D-galactose
-
2,6-dichlorophenol indophenol used as electron acceptor
91
D-galactose
-
O2 used as electron acceptor
96
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
110
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
132
D-galactose
wild type enzyme, at 37°C and pH 8.0
3.11
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
5.4
D-glucose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
8.1
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
8.1
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37°C
8.1
D-glucose
pH 8.0, 37°C, recombinant mutant Y300A
12
D-glucose
mutant enzyme A38V, at pH 8.0 and 37°C
15
D-glucose
wild type enzyme, at pH 8.0 and 37°C
15
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
17.4
D-glucose
wild type enzyme, at 37°C and pH 8.0
20
D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
25
D-glucose
-
O2 used as electron acceptor
31
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
2 - 3
D-xylose
-
O2 used as electron acceptor
6.7
D-xylose
-
2,6-dichlorophenol indophenol used as electron acceptor
32
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
37
D-xylose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
51.8
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
51.8
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37°C
51.8
D-xylose
pH 8.0, 37°C, recombinant mutant Y300A
105
D-xylose
wild type enzyme, at 37°C and pH 8.0
118
D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
118
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
129
D-xylose
wild type enzyme, at pH 8.0 and 37°C
288
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
47
L-arabinose
-
O2 used as electron acceptor
85
L-arabinose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.11
lactose
-
O2 used as electron acceptor
0.19
lactose
-
2,6-dichlorophenol indophenol used as electron acceptor
13
maltohexaose
-
O2 used as electron acceptor
30
maltohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
11
maltopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
32
maltopentaose
-
O2 used as electron acceptor
2.81
maltose
wild type enzyme, at 37°C and pH 8.0
4.9
maltose
-
2,6-dichlorophenol indophenol used as electron acceptor
5
maltose
mutant enzyme W351F, at 37°C and pH 8.0
11
maltose
-
O2 used as electron acceptor
11
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
19.6
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
12
maltotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
30
maltotetraose
-
O2 used as electron acceptor
18
maltotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
30
maltotriose
-
O2 used as electron acceptor
56
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
92
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
340
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
950
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
0.05
xylobiose
mutant enzyme A38V, at pH 8.0 and 37°C
0.057
xylobiose
wild type enzyme, at pH 8.0 and 37°C
0.09
xylobiose
-
O2 used as electron acceptor
0.098
xylobiose
wild type enzyme, at 37°C and pH 8.0
0.099
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.21
xylobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.35
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
4.83
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
5.11
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
5.11
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
5.11
xylobiose
pH 8.0, 37°C, recombinant mutant Y300A
5.84
xylobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.005
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
xylohexaose
wild type enzyme, at pH 8.0 and 37°C
0.1
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
3
xylohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.05
xylopentaose
wild type enzyme, at pH 8.0 and 37°C
0.05
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.104
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
4.49
xylopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.067
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
0.07
xylotetraose
wild type enzyme, at pH 8.0 and 37°C
0.11
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.08
xylotriose
wild type enzyme, at pH 8.0 and 37°C
0.08
xylotriose
mutant enzyme A38V, at pH 8.0 and 37°C
0.1
xylotriose
wild type enzyme, at 37°C and pH 8.0
0.1
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.31
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
3.15
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
3.15
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
3.15
xylotriose
pH 8.0, 37°C, recombinant mutant Y300A
4.3
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
4.57
xylotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.45 - 0.81
6-deoxy-D-glucose
9 - 13.5
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
8.8 - 12.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
1.1 - 18.33
cellopentaose
7.8 - 12.8
N-acetyl-D-glucosamine
0.45
6-deoxy-D-glucose
-
O2 used as electron acceptor
0.81
6-deoxy-D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
9
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
13.5
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
8.8
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
12.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.22
cellobiose
H70A mutant protein
0.58
cellobiose
C130A mutant protein
5.7
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
6.2
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
6.3
cellobiose
wild type enzyme, at 37°C and pH 8.0
7
cellobiose
wild type enzyme, at pH 8.0 and 37°C
7.4
cellobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
9
cellobiose
-
O2 used as electron acceptor
11.4
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
11.9
cellobiose
wild type protein
12
cellobiose
mutant enzyme A38V, at pH 8.0 and 37°C
13.7
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
13.7
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
13.72
cellobiose
pH 8.0, 37°C, recombinant mutant Y300A
16.33
cellobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.1
cellohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
6.6
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
7.2
cellohexaose
wild type enzyme, at pH 8.0 and 37°C
10
cellohexaose
-
O2 used as electron acceptor
13.2
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
16.5
cellohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.1
cellopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
7.6
cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
8.3
cellopentaose
wild type enzyme, at pH 8.0 and 37°C
9
cellopentaose
-
O2 used as electron acceptor
12.7
cellopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
18.33
cellopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.4
cellotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
4.17
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
7.8
cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
9.9
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
11
cellotetraose
-
O2 used as electron acceptor
12.8
cellotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
1.5
cellotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
5.3
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
6
cellotriose
wild type enzyme, at 37°C and pH 8.0
6.9
cellotriose
wild type enzyme, at pH 8.0 and 37°C
7.3
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
7.5
cellotriose
-
O2 used as electron acceptor
10
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
11.17
cellotriose
pH 8.0, 37°C, recombinant mutant Y300A
11.2
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
11.2
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
13.7
cellotriose
mutant enzyme A38V, at pH 8.0 and 37°C
18.67
cellotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.25
D-galactose
-
2,6-dichlorophenol indophenol used as electron acceptor
1.5
D-galactose
-
O2 used as electron acceptor
6.6
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
7.2
D-galactose
wild type enzyme, at 37°C and pH 8.0
11.8
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
13.3
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
0.46
D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
2.1
D-glucose
-
O2 used as electron acceptor
5.6
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
6
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
6.3
D-glucose
wild type enzyme, at pH 8.0 and 37°C
7.5
D-glucose
wild type enzyme, at 37°C and pH 8.0
10.4
D-glucose
mutant enzyme A38V, at pH 8.0 and 37°C
10.8
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
13.2
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
13.2
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37°C
13.22
D-glucose
pH 8.0, 37°C, recombinant mutant Y300A
15.68
D-glucose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.58
D-xylose
-
2,6-dichlorophenol indophenol used as electron acceptor
1.7
D-xylose
-
O2 used as electron acceptor
4.6
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
5.3
D-xylose
wild type enzyme, at 37°C and pH 8.0
5.5
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
7.4
D-xylose
wild type enzyme, at pH 8.0 and 37°C
11.3
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
11.3
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37°C
11.33
D-xylose
pH 8.0, 37°C, recombinant mutant Y300A
11.7
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
12.4
D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
16.25
D-xylose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.73
L-arabinose
-
O2 used as electron acceptor
1.1
L-arabinose
-
2,6-dichlorophenol indophenol used as electron acceptor
4.4
lactose
-
2,6-dichlorophenol indophenol used as electron acceptor
6.3
lactose
-
O2 used as electron acceptor
0.35
maltohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.57
maltohexaose
-
O2 used as electron acceptor
0.9
maltopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
1.8
maltopentaose
-
O2 used as electron acceptor
0.76
maltose
-
2,6-dichlorophenol indophenol used as electron acceptor
2
maltose
-
O2 used as electron acceptor
5.4
maltose
mutant enzyme W351F, at 37°C and pH 8.0
6
maltose
wild type enzyme, at 37°C and pH 8.0
10.4
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
10.4
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
2.3
maltotetraose
-
O2 used as electron acceptor
2.6
maltotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
2.2
maltotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
2.3
maltotriose
-
O2 used as electron acceptor
7.8
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
8.1
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
11.3
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
12.8
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
3
xylobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
3.3
xylobiose
-
O2 used as electron acceptor
7.5
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
8
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
8.7
xylobiose
wild type enzyme, at pH 8.0 and 37°C
8.8
xylobiose
wild type enzyme, at 37°C and pH 8.0
12
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
13.28
xylobiose
pH 8.0, 37°C, recombinant mutant Y300A
13.3
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
13.3
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
14.8
xylobiose
mutant enzyme A38V, at pH 8.0 and 37°C
19.72
xylobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
7.8
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
8.6
xylohexaose
wild type enzyme, at pH 8.0 and 37°C
15.2
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
17.43
xylohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
8.8
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
10.4
xylopentaose
wild type enzyme, at pH 8.0 and 37°C
15.2
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
26.17
xylopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
8.1
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
9.7
xylotetraose
wild type enzyme, at pH 8.0 and 37°C
16
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
7.9
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
8.3
xylotriose
wild type enzyme, at 37°C and pH 8.0
8.7
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
10.3
xylotriose
wild type enzyme, at pH 8.0 and 37°C
12
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
13.87
xylotriose
pH 8.0, 37°C, recombinant mutant Y300A
13.9
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
13.9
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
14.5
xylotriose
mutant enzyme A38V, at pH 8.0 and 37°C
19.67
xylotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
36.5
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
115 - 150
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
148.3 - 233.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
78.3 - 216.7
cellopentaose
56.7 - 233.3
cellotetraose
0.008 - 0.2
N-acetyl-D-glucosamine
73.3 - 233.3
xylotetraose
115
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
150
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
148.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
233.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.25
cellobiose
pH 8.0, 37°C, recombinant mutant Y300A
30
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
56.7
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
56.7
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
68.3
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
100
cellobiose
wild type enzyme, at 37°C and pH 8.0
116.7
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
156.7
cellobiose
wild type enzyme, at pH 8.0 and 37°C
306.7
cellobiose
mutant enzyme A38V, at pH 8.0 and 37°C
66.7
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
91.7
cellohexaose
wild type enzyme, at pH 8.0 and 37°C
150
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
78.3
cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
145
cellopentaose
wild type enzyme, at pH 8.0 and 37°C
216.7
cellopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
56.7
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
86.7
cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
150
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
233.3
cellotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
0.25
cellotriose
pH 8.0, 37°C, recombinant mutant Y300A
23.3
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
45
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
45
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
48.3
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
70
cellotriose
wild type enzyme, at 37°C and pH 8.0
93.3
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
143.3
cellotriose
wild type enzyme, at pH 8.0 and 37°C
250
cellotriose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
D-galactose
wild type enzyme, at 37°C and pH 8.0
0.11
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
0.14
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
0.18
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
0.18
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
0.4
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.42
D-glucose
wild type enzyme, at pH 8.0 and 37°C
0.43
D-glucose
wild type enzyme, at 37°C and pH 8.0
0.83
D-glucose
mutant enzyme A38V, at pH 8.0 and 37°C
1.6
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37°C
1.63
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
2.883
D-glucose
pH 8.0, 37°C, recombinant enzyme mutant
3.6
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
8.1
D-glucose
pH 8.0, 37°C, recombinant mutant Y300A
0.02
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
0.05
D-xylose
wild type enzyme, at 37°C and pH 8.0
0.05
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.06
D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.11
D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.22
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
0.22
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37°C
0.36
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
0.45
D-xylose
pH 8.0, 37°C, recombinant enzyme mutant
51.8
D-xylose
pH 8.0, 37°C, recombinant mutant Y300A
0.53
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
0.95
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
1.1
maltose
mutant enzyme W351F, at 37°C and pH 8.0
2.13
maltose
wild type enzyme, at 37°C and pH 8.0
0.008
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
0.02
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
0.14
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
0.2
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
2.48
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
2.6
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
2.6
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
5.11
xylobiose
pH 8.0, 37°C, recombinant mutant Y300A
23.3
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
75
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
90
xylobiose
wild type enzyme, at 37°C and pH 8.0
151.7
xylobiose
wild type enzyme, at pH 8.0 and 37°C
300
xylobiose
mutant enzyme A38V, at pH 8.0 and 37°C
73.3
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
135
xylohexaose
wild type enzyme, at pH 8.0 and 37°C
300
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
85
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
196.7
xylopentaose
wild type enzyme, at pH 8.0 and 37°C
283.3
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
73.3
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
136.7
xylotetraose
wild type enzyme, at pH 8.0 and 37°C
233.3
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
2.83
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
3.15
xylotriose
pH 8.0, 37°C, recombinant mutant Y300A
4.3
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
4.33
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
25
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
85
xylotriose
wild type enzyme, at 37°C and pH 8.0
85
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
136.7
xylotriose
wild type enzyme, at pH 8.0 and 37°C
183.3
xylotriose
mutant enzyme A38V, at pH 8.0 and 37°C
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A38V
the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
A38V/S388N
the mutation increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
C130A
FAD binding site, FAD covalently attached
E247A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
H70A
FAD binding site, FAD covalently attached
H70A/C130A
no activity, lack of essential FAD cofactor
Q353A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Q353N
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Q384A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Q384N
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
W351F
the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates
Y300N
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
Y310A
larger amount of carbohydrates
Y72A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Y72F
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
A38V
-
the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
-
E247A
-
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
-
Q384A
-
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
-
W351F
-
the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates
-
Y300A
-
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
-
Y300N
-
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
-
Y72A
-
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
-
Y72F
-
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
-
W351A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
W351A
the mutation increases Km values by up to 2 orders of magnitude while also increasing kcat up to 3fold on cello- and xylo-oligosaccharides and showing no substrate inhibition compared to the wild type enzyme
Y300A
the mutation doubles kcat values for monosaccharide and oligosaccharide substrates
Y300A
the variant shows broader substrate range and higher H2O2 stability and specifically exhibits up to 40times higher activity on all tested sugars except D-glucose compared to the wild type enzyme
Y300A
the mutant variant of gluco-oligosaccharide oxidase (GOOX) from Sarocladium strictum shows a broader substrate range and higher H2O2 stability compared to the wild-type enzyme. The mutant Y300A exhibits up to 40times higher activity on all tested sugars except glucose, compared to wild-type. Fusion of the Y300A variant to a family 22 carbohydrate binding module from Clostridium thermocellum (CtCBM22A) nearly doubles its catalytic efficiency on glucose, while retaining significant activity on oligosaccharides. In the presence of 200 mM of H2O2, the recombinant CtCBM22A_Y300A retains 80% of activity on glucose and 100% of activity on cellobiose, the preferred substrate for this enzyme, while the wild-type enzyme retains 60% activity on D-glucose under the same conditions. GOOX variants appear to undergo a different mechanism of inactivation, as a loss of histidine instead of methionine is observed after H2O2 incubation. The addition of CtCBM22A also promotes functional binding of the fusion enzyme to xylan, facilitating its simultaneous purification and immobilization using edible oat spelt xylan, which might benefit the usage of this enzyme preparation in food and baking applications. The presence of CtCBM22A also permits enzyme binding to oat spelt xylans, facilitating simultaneous purification and immobilization of the enzyme, and ancillary fibre enrichment in potential food applications
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Kiryu, T.; Nakano, H.; Kiso, T.; Murakami, H.
Purification and characterization of a carbohydrate: acceptor oxidoreductase from Paraconiothyrium sp. that produces lactobionic acid efficiently
Biosci. Biotechnol. Biochem.
72
833-841
2008
Paraconiothyrium sp.
brenda
Huang, C.H.; Winkler, A.; Chen, C.L.; Lai, W.L.; Tsai, Y.C.; Macheroux, P.; Liaw, S.H.
Functional roles of the 6-S-cysteinyl, 8alpha-N1-histidyl FAD in glucooligosaccharide oxidase from Acremonium strictum
J. Biol. Chem.
283
30990-30996
2008
Sarocladium strictum (Q6PW77), Sarocladium strictum
brenda
Foumani, M.; Vuong, T.V.; Master, E.R.
Altered substrate specificity of the gluco-oligosaccharide oxidase from Acremonium strictum
Biotechnol. Bioeng.
108
2261-2269
2011
Sarocladium strictum (Q6PW77), Sarocladium strictum, Sarocladium strictum CBS 346.70 (Q6PW77)
brenda
Vuong, T.V.; Vesterinen, A.H.; Foumani, M.; Juvonen, M.; Seppaelae, J.; Tenkanen, M.; Master, E.R.
Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictum and variants with reduced substrate inhibition
Biotechnol. Biofuels
6
148
2013
Sarocladium strictum (D7PF15), Sarocladium strictum, Sarocladium strictum CBS 346.70 (D7PF15)
brenda
Foumani, M.; Vuong, T.V.; MacCormick, B.; Master, E.R.
Enhanced polysaccharide binding and activity on linear beta-glucans through addition of carbohydrate-binding modules to either terminus of a glucooligosaccharide oxidase
PLoS ONE
10
e0125398
2015
Sarocladium strictum (D7PF15), Sarocladium strictum CBS 346.70 (D7PF15), Sarocladium strictum CBS 346.70
brenda
Vuong, T.V.; Master, E.R.
Fusion of a xylan-binding module to gluco-oligosaccharide oxidase increases activity and promotes stable immobilization
PLoS ONE
9
e95170
2014
Sarocladium strictum (D7PF15), Sarocladium strictum, Sarocladium strictum CBS 346.70 (D7PF15)
brenda
Vuong, T.V.; Foumani, M.; MacCormick, B.; Kwan, R.; Master, E.R.
Direct comparison of gluco-oligosaccharide oxidase variants and glucose oxidase: substrate range and H2O2 stability
Sci. Rep.
6
37356
2016
Sarocladium strictum (D7PF15)
brenda
Vuong, T.V.; Foumani, M.; MacCormick, B.; Kwan, R.; Master, E.R.
Direct comparison of gluco-oligosaccharide oxidase variants and glucose oxidase substrate range and H2O2 stability
Sci. Rep.
6
37356
2016
Sarocladium strictum (Q6PW77)
brenda