Information on EC 1.1.5.14 - fructose 5-dehydrogenase

for references in articles please use BRENDA:EC1.1.5.14

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EC Tree 1 Oxidoreductases 1.1 Acting on the CH-OH group of donors 1.1.5 With a quinone or similar compound as acceptor 1.1.5.14 fructose 5-dehydrogenase

IUBMB Comments

The enzyme, characterized from the bacterium Gluconobacter japonicus, is a heterotrimer composed of an FAD-containing large subunit, a small subunit, and a heme c-containing subunit, which is responsible for anchoring the complex to the cytoplasmic membrane and for transferring the electrons to ubiquinone.

The enzyme appears in viruses and cellular organisms

1.1.5.14
electrode
biosensors
amperometric
gluconobacter
5-ketofructose
oxydans
biocathode
bioelectrode
bioanode
tetrathiafulvalene
5-keto-d-fructose
biotechnology
food industry
analysis
synthesis
biofuel production
industry

Reaction Schemes

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an ubiquinone

an ubiquinol

Synonyms
fructose dehydrogenase, d-fructose dehydrogenase, fdhscl, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D-fructose dehydrogenase

11 entries

dehydrogenase, fructose 5- (acceptor)

FDH

FdhL

FdhS

fdhSCL

fructose 5-dehydrogenase

Gluconobacter sp.

686063

fructose dehydrogenase

4 entries

D-fructose dehydrogenase

Gluconobacter frateurii

710853, 762826

D-fructose dehydrogenase

Gluconobacter frateurii CHM 43

D-fructose dehydrogenase

Gluconobacter japonicus

740360, 740361, 740362, 740364, 740368, 740369, 760332, 760656, 760974, 760975, 760976, 760977, 761572, 761604

D-fructose dehydrogenase

Gluconobacter japonicus LMG 1281

D-fructose dehydrogenase

Gluconobacter japonicus NBRC 3260

D-fructose dehydrogenase

Gluconobacter japonicus NBRC3260

D-fructose dehydrogenase

Gluconobacter japonicus NCBR 3260

D-fructose dehydrogenase

Gluconobacter oxydans

703276

D-fructose dehydrogenase

Gluconobacter sp.

706060, 723937, 724735

D-fructose dehydrogenase

Gluconobacter sp.

FDH

686063

FDH

Gluconobacter frateurii

710853, 762826

FDH

Gluconobacter frateurii CHM 43

FDH

Gluconobacter japonicus

724031, 739769, 739849, 740360, 740361, 740362, 740364, 740368, 740369, 760332, 760337, 760973, 760974, 760975, 760976, 760977, 761572, 761604

FDH

Gluconobacter japonicus LMG 1281

FDH

Gluconobacter japonicus NBRC 3260

FDH

Gluconobacter japonicus NBRC3260

FDH

Gluconobacter japonicus NBRCA3260

FDH

Gluconobacter japonicus NCBR 3260

FDH

Gluconobacter oxydans

740366

FDH

Gluconobacter oxydans 621H

FDH

Gluconobacter sp.

655081, 702780, 723937, 724735, 739767, 740365, 760893, 762362

FdhL

Gluconobacter japonicus

large subunit

724031

FdhL

Gluconobacter japonicus NBRC3260

large subunit

FdhS

Gluconobacter japonicus

small subunit

724031

FdhS

Gluconobacter japonicus NBRC3260

small subunit

fdhSCL

Gluconobacter japonicus

gene name of the flavoprotein-cytochrome c complex fructose dehydrogenase

724031

fdhSCL

Gluconobacter japonicus NBRC3260

gene name of the flavoprotein-cytochrome c complex fructose dehydrogenase

fructose dehydrogenase

Gluconobacter japonicus

724031, 760337, 760973

fructose dehydrogenase

Gluconobacter japonicus NBRC3260

fructose dehydrogenase

Gluconobacter japonicus NCBR 3260

fructose dehydrogenase

Gluconobacter sp.

702780, 760893, 762362

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

D-fructose + acceptor = 5-dehydro-D-fructose + reduced acceptor

2 entries

D-fructose + an ubiquinone = 5-dehydro-D-fructose + an ubiquinol

D-fructose + acceptor = 5-dehydro-D-fructose + reduced acceptor

ping-pong mechanism

Gluconobacter sp.

348284

D-fructose + acceptor = 5-dehydro-D-fructose + reduced acceptor

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

oxidation

redox reaction

reduction

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SYSTEMATIC NAME

IUBMB Comments

D-fructose:ubiquinone 5-oxidoreductase

The enzyme, characterized from the bacterium Gluconobacter japonicus, is a heterotrimer composed of an FAD-containing large subunit, a small subunit, and a heme c-containing subunit, which is responsible for anchoring the complex to the cytoplasmic membrane and for transferring the electrons to ubiquinone.

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CAS REGISTRY NUMBER

COMMENTARY

37250-85-4

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

D-(-)fructose + 2,6-dichloroindophenol

5-dehydro-D-(-)-fructose + reduced 2,6-dichloroindophenol

2 entries

D-(-)fructose + cytochrome c

5-dehydro-D-(-)-fructose + reduced cytochrome c

2 entries

D-(-)fructose + potassium ferricyanide

5-dehydro-D-(-)-fructose + potassium ferrocyanide

2 entries

D-fructose + 1,1'-dimethylferricenium ion

5-dehydro-D-fructose + 1,1'-dimethylferrocenium ion

Gluconobacter sp.

723937

Substrates: -
Products: -

D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone

5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol

2 entries

D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone

5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol

2 entries

D-fructose + 2,5-bis[methyl(phenyl)amino]cyclohexa-2,5-diene-1,4-dione

5-dehydro-D-fructose + 2,5-bis[methyl(phenyl)amino]benzene-1,4-diol

Gluconobacter japonicus

760973

Substrates: -
Products: -

D-fructose + 2,6-dichlorophenolindophenol

5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol

2 entries

D-fructose + 2-(2-fluoroanilino)cyclohexa-2,5-diene-1,4-dione

5-dehydro-D-fructose + 2-(2-fluoroanilino)benzene-1,4-diol

Gluconobacter japonicus

760973

Substrates: -
Products: -

D-fructose + 2-(2-methoxyanilino)cyclohexa-2,5-diene-1,4-dione

5-dehydro-D-fructose + 2-(2-methoxyanilino)benzene-1,4-diol

Gluconobacter japonicus

760973

Substrates: -
Products: -

D-fructose + 2-(3-nitroanilino)cyclohexa-2,5-diene-1,4-dione

5-dehydro-D-fructose + 2-(3-nitroanilino)benzene-1,4-diol

Gluconobacter japonicus

760973

Substrates: -
Products: -

D-fructose + 2-(4-fluoroanilino)cyclohexa-2,5-diene-1,4-dione

5-dehydro-D-fructose + 2-(4-fluoroanilino)benzene-1,4-diol

Gluconobacter japonicus

760973

Substrates: -
Products: -

D-fructose + 2-(4-methoxyanilino)cyclohexa-2,5-diene-1,4-dione

5-dehydro-D-fructose + 2-(4-methoxyanilino)benzene-1,4-diol

Gluconobacter japonicus

760973

Substrates: -
Products: -

D-fructose + 2-[methyl(phenyl)amino]cyclohexa-2,5-diene-1,4-dione

5-dehydro-D-fructose + 2-[methyl(phenyl)amino]benzene-1,4-diol

Gluconobacter japonicus

760973

Substrates: -
Products: -

D-fructose + 7,7,8,8-tetracyanoquinodimethane

5-dehydro-D-fructose + reduced 7,7,8,8-tetracyanoquinodimethane

Gluconobacter sp.

724735

Substrates: -
Products: -

D-fructose + acceptor

2-dehydro-D-fructose + reduced acceptor

Gluconobacter sp.

686063

Substrates: direct electron-transfer bioelectrocatalytic oxidation, DET-type
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

4 entries

D-fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

2 entries

D-fructose + electron acceptor

5-dehydro-D-fructose + reduced electron acceptor

Gluconobacter sp.

762362

Substrates: -
Products: -

D-fructose + ferricyanide

5-dehydro-D-fructose + ferrocyanide

6 entries

D-fructose + nitro blue tetrazolium

5-dehydro-D-fructose + ?

Gluconobacter oxydans

348281, 348283

Substrates: -
Products: -

D-fructose + oxidized 1-methoxy-5-methylphenazinium methylsulfate

5-dehydro-D-fructose + reduced 1-methoxy-5-methylphenazinium methylsulfate

Gluconobacter oxydans

703276

Substrates: -
Products: -

D-fructose + oxidized electron acceptor

5-dehydro-D-fructose + reduced electron acceptor

2 entries

D-fructose + phenazine methosulfate

5-dehydro-D-fructose + ?

Gluconobacter oxydans

348281, 348283

Substrates: -
Products: -

D-fructose + potassium ferricyanide

5-dehydro-D-fructose + potassium ferrocyanide

4 entries

D-fructose + ubiquinone

5-dehydro-D-fructose + ubiquinol

2 entries

D-fructose + [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]

5-dehydro-D-fructose + [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] formazan

Gluconobacter sp.

706060

Substrates: in the presence of phenazine methosulfate
Products: -

D-fructose + [Fe(CN)6]3-

5-dehydro-D-fructose + [Fe(CN)6]4-

Gluconobacter sp.

655081

Substrates: -
Products: -

fructose + 2,6-dichlorophenolindophenol

5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol

2 entries

fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

2 entries

fructose + phenazine methosulfate

5-dehydro-D-fructose + reduced phenazine methosulfate

2 entries

additional information

14 entries

D-(-)fructose + 2,6-dichloroindophenol

5-dehydro-D-(-)-fructose + reduced 2,6-dichloroindophenol

Gluconobacter japonicus

760332

Substrates: -
Products: -

D-(-)fructose + 2,6-dichloroindophenol

5-dehydro-D-(-)-fructose + reduced 2,6-dichloroindophenol

Gluconobacter japonicus NCBR 3260

760332

Substrates: -
Products: -

D-(-)fructose + cytochrome c

5-dehydro-D-(-)-fructose + reduced cytochrome c

Gluconobacter japonicus

760332

Substrates: -
Products: -

D-(-)fructose + cytochrome c

5-dehydro-D-(-)-fructose + reduced cytochrome c

Gluconobacter japonicus NCBR 3260

760332

Substrates: -
Products: -

D-(-)fructose + potassium ferricyanide

5-dehydro-D-(-)-fructose + potassium ferrocyanide

Gluconobacter japonicus

760332

Substrates: -
Products: -

D-(-)fructose + potassium ferricyanide

5-dehydro-D-(-)-fructose + potassium ferrocyanide

Gluconobacter japonicus NCBR 3260

760332

Substrates: -
Products: -

D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone

5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol

Gluconobacter japonicus

724031

Substrates: -
Products: -

D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone

5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol

Gluconobacter japonicus NBRC3260

724031

Substrates: -
Products: -

D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone

5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol

Gluconobacter japonicus

724031

Substrates: -
Products: -

D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone

5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol

Gluconobacter japonicus NBRC3260

724031

Substrates: -
Products: -

D-fructose + 2,6-dichlorophenolindophenol

5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol

Gluconobacter cerinus

348282

Substrates: most effective electron acceptor
Products: -

D-fructose + 2,6-dichlorophenolindophenol

5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol

Gluconobacter oxydans

348281, 348283

Substrates: -
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter frateurii

710853

Substrates: -
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter japonicus

739769, 740362, 740361, 740364

Substrates: -
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter oxydans

348283

Substrates: involved in utilization of D-fructose by acetic acid bacteria
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter sp.

702780, 740365, 739767

Substrates: -
Products: -

D-fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus

760976

Substrates: -
Products: -

D-fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus NBRC 3260

760976

Substrates: -
Products: -

D-fructose + ferricyanide

5-dehydro-D-fructose + ferrocyanide

Gluconobacter japonicus

739849, 740360, 740368

Substrates: -
Products: -

D-fructose + ferricyanide

5-dehydro-D-fructose + ferrocyanide

Gluconobacter japonicus NBRC3260

740360, 740368

Substrates: -
Products: -

D-fructose + ferricyanide

5-dehydro-D-fructose + ferrocyanide

Gluconobacter japonicus NBRCA3260

739849

Substrates: -
Products: -

D-fructose + ferricyanide

5-dehydro-D-fructose + ferrocyanide

Gluconobacter oxydans

348281, 348283, 740366

Substrates: -
Products: -

D-fructose + ferricyanide

5-dehydro-D-fructose + ferrocyanide

Gluconobacter oxydans 621H

740366

Substrates: -
Products: -

D-fructose + ferricyanide

5-dehydro-D-fructose + ferrocyanide

Gluconobacter sp.

348284

Substrates: -
Products: -

D-fructose + oxidized electron acceptor

5-dehydro-D-fructose + reduced electron acceptor

Gluconobacter japonicus

760656

Substrates: -
Products: -

D-fructose + oxidized electron acceptor

5-dehydro-D-fructose + reduced electron acceptor

Gluconobacter sp.

760893

Substrates: -
Products: -

D-fructose + potassium ferricyanide

5-dehydro-D-fructose + potassium ferrocyanide

Gluconobacter japonicus

761604, 760337, 760974, 760975, 760977

Substrates: -
Products: -

D-fructose + potassium ferricyanide

5-dehydro-D-fructose + potassium ferrocyanide

Gluconobacter japonicus NBRC 3260

761604

Substrates: -
Products: -

D-fructose + potassium ferricyanide

5-dehydro-D-fructose + potassium ferrocyanide

Gluconobacter japonicus NBRC3260

760975, 760977

Substrates: -
Products: -

D-fructose + potassium ferricyanide

5-dehydro-D-fructose + potassium ferrocyanide

Gluconobacter japonicus NCBR 3260

760337, 760974

Substrates: -
Products: -

D-fructose + ubiquinone

5-dehydro-D-fructose + ubiquinol

Gluconobacter japonicus

740369

Substrates: -
Products: -

D-fructose + ubiquinone

5-dehydro-D-fructose + ubiquinol

Gluconobacter japonicus NBRC3260

740369

Substrates: -
Products: -

fructose + 2,6-dichlorophenolindophenol

5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol

Gluconobacter japonicus

761572

Substrates: -
Products: -

fructose + 2,6-dichlorophenolindophenol

5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol

Gluconobacter japonicus LMG 1281

761572

Substrates: -
Products: -

fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus

761572

Substrates: -
Products: -

fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus LMG 1281

761572

Substrates: -
Products: -

fructose + phenazine methosulfate

5-dehydro-D-fructose + reduced phenazine methosulfate

Gluconobacter japonicus

761572

Substrates: -
Products: -

fructose + phenazine methosulfate

5-dehydro-D-fructose + reduced phenazine methosulfate

Gluconobacter japonicus LMG 1281

761572

Substrates: -
Products: -

additional information

Gluconobacter cerinus

348282

Substrates: no activity with NADP+
Products: -

additional information

Gluconobacter cerinus

348282

Substrates: no activity with NAD+
Products: -

additional information

Gluconobacter frateurii

710853

Substrates: oxygen is completely inactive as an electron acceptor
Products: -

additional information

Gluconobacter frateurii

710853

Substrates: when FDH is adsorbed onto a porous carbon electrode surface, it can transfer electrons from D-fructose to the electrode directly without a mediator
Products: -

additional information

Gluconobacter japonicus

724031

Substrates: cells producing only subunits FdhS and FdhL have no fructose-oxidizing activity, but show significantly high D-fructose:ferricyanide oxidoreductase activity in the soluble fraction of cell extracts, whereas the cells producing the FDH complex show activity in the membrane fraction
Products: -

additional information

Gluconobacter japonicus

760332

Substrates: subunit II is equivalent to the cytochrome domain and acts as electron acceptor to the catalytic subunit I of the heterotrimeric enzyme
Products: -

additional information

Gluconobacter japonicus NBRC3260

724031

additional information

Gluconobacter japonicus NCBR 3260

760332

Substrates: subunit II is equivalent to the cytochrome domain and acts as electron acceptor to the catalytic subunit I of the heterotrimeric enzyme
Products: -

additional information

Gluconobacter oxydans

348281

Substrates: no activity with NADP+
Products: -

additional information

Gluconobacter oxydans

348283

Substrates: no activity with NADP+
Products: -

additional information

Gluconobacter oxydans

348281

Substrates: no activity with O2
Products: -

additional information

Gluconobacter oxydans

348283

Substrates: no activity with O2
Products: -

additional information

Gluconobacter oxydans

348281

Substrates: no activity with NAD+
Products: -

additional information

Gluconobacter oxydans

348283

Substrates: no activity with NAD+
Products: -

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

4 entries

D-fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

2 entries

D-fructose + oxidized electron acceptor

5-dehydro-D-fructose + reduced electron acceptor

2 entries

D-fructose + ubiquinone

5-dehydro-D-fructose + ubiquinol

2 entries

fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

2 entries

additional information

Gluconobacter frateurii

710853

Substrates: oxygen is completely inactive as an electron acceptor
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter frateurii

710853

Substrates: -
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter japonicus

739769, 740362, 740361, 740364

Substrates: -
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter oxydans

348283

Substrates: involved in utilization of D-fructose by acetic acid bacteria
Products: -

D-fructose + acceptor

5-dehydro-D-fructose + reduced acceptor

Gluconobacter sp.

740365, 739767

Substrates: -
Products: -

D-fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus

760976

Substrates: -
Products: -

D-fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus NBRC 3260

760976

Substrates: -
Products: -

D-fructose + oxidized electron acceptor

5-dehydro-D-fructose + reduced electron acceptor

Gluconobacter japonicus

760656

Substrates: -
Products: -

D-fructose + oxidized electron acceptor

5-dehydro-D-fructose + reduced electron acceptor

Gluconobacter sp.

760893

Substrates: -
Products: -

D-fructose + ubiquinone

5-dehydro-D-fructose + ubiquinol

Gluconobacter japonicus

740369

Substrates: -
Products: -

D-fructose + ubiquinone

5-dehydro-D-fructose + ubiquinol

Gluconobacter japonicus NBRC3260

740369

Substrates: -
Products: -

fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus

761572

Substrates: -
Products: -

fructose + an ubiquinone

5-dehydro-D-fructose + an ubiquinol

Gluconobacter japonicus LMG 1281

761572

Substrates: -
Products: -

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

cytochrome c

FAD

heme

heme c

pyrroloquinoline quinone

Gluconobacter sp.

quinohemoprotein. Enzyme has two active centers: cytochrome c and pyrroloquinoline quinone

348284

cytochrome c

Gluconobacter oxydans

enzyme contains a cytochrome c of 50800 Da

348281

cytochrome c

Gluconobacter oxydans

contains a cytochrome c like electron carrier, tightly bound

348283

cytochrome c

Gluconobacter japonicus

the cytochrome c subunit is responsible not only for membrane anchoring but also for ubiquinone reduction

724031

cytochrome c

Gluconobacter japonicus

cytochrome c

FAD

FAD

Gluconobacter japonicus

739769

FAD

Gluconobacter oxydans

740366

FAD

Gluconobacter japonicus

739849, 740362, 740364, 740369

FAD

Gluconobacter japonicus

subunit I contains three FAD molecules

740368

FAD

Gluconobacter japonicus

761572, 761604

FAD

Gluconobacter sp.

762362

FAD

Gluconobacter japonicus

760332, 760337, 760656

FAD

Gluconobacter sp.

760893

FAD

Gluconobacter japonicus

760973, 760974, 760975, 760977, 760976

heme

Gluconobacter sp.

quinohemoprotein. Enzyme has two active centers: cytochrome c and pyrroloquinoline quinone

348284

heme

Gluconobacter japonicus

the enzyme has three heme C moieties

724031

heme

Gluconobacter japonicus

heme c

heme c

the enzyme contains three hemes c

724735

heme c

Gluconobacter oxydans

740366

heme c

Gluconobacter japonicus

739849, 740360, 740362, 740364, 740369

heme c

Gluconobacter japonicus

subunit II contains three heme C moieties

740368

heme c

Gluconobacter japonicus

761604

heme c

Gluconobacter sp.

762362

heme c

Gluconobacter japonicus

760332, 760337

heme c

Gluconobacter sp.

760893

heme c

Gluconobacter japonicus

760974, 760975

heme c

Gluconobacter japonicus

the enzyme has has three hemes c in subunit II

760977

heme c

Gluconobacter japonicus

the enzyme contains three heme c moieties

760976

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ca2+

2 entries

Mg2+

Gluconobacter japonicus

the addition of Mg2+ has an increasing effect on the catalytic efficiency of the enzyme

760332

Ca2+

Gluconobacter japonicus

activates

739769

Ca2+

Gluconobacter japonicus

the addition of Mg2+ has an increasing effect on the catalytic efficiency of the enzyme

760332

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

Ag+

Gluconobacter cerinus

348282

Atebrine

Gluconobacter cerinus

slight

348282

Cd2+

Gluconobacter cerinus

348282

Cu2+

Gluconobacter cerinus

348282

cyanide

Gluconobacter japonicus

760977

EDTA

Gluconobacter cerinus

slight

348282

Hg2+

Gluconobacter cerinus

348282

o-phenanthroline

Gluconobacter cerinus

slight

348282

p-chloromercuribenzoate

Gluconobacter cerinus

348282

Phenylmercuric nitrate

Gluconobacter cerinus

348282

Zn2+

Gluconobacter cerinus

348282

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2.6 - 11

D-fructose

5 entries

0.0011 - 0.47

ferricyanide

2 entries

4.2 - 11.8

fructose

2 entries

2.6

D-fructose

Gluconobacter japonicus

at pH 5.0 and 37°C

739849

D-fructose

Gluconobacter oxydans

348281, 348283

D-fructose

Gluconobacter cerinus

in presence of 2,6-dichlorophenolindophenol, 67 mM, pH 5.5, 15°C

348282

D-fructose

Gluconobacter sp.

irreversibly adsorbed on Ketjen black - modified glassy carbon electrodes

686063

D-fructose

Gluconobacter sp.

Km is determined using FDH adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode

702780

0.0011

ferricyanide

Gluconobacter sp.

slow reaction phase

348284

0.47

ferricyanide

Gluconobacter sp.

slow reaction phase

348284

4.2

fructose

Gluconobacter sp.

slow reaction phase

348284

11.8

fructose

Gluconobacter sp.

fast reaction phase

348284

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

95 - 250

D-fructose

2 entries

93 - 162

ferricyanide

2 entries

D-fructose

Gluconobacter sp.

slow reaction phase

348284

250

D-fructose

fast reaction phase

ferricyanide

slow reaction phase

162

ferricyanide

Gluconobacter sp.

fast reaction phase

348284

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

172

Gluconobacter oxydans

348281, 348283

additional information

Gluconobacter cerinus

348282

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3 entries

4 - 4.5

2 entries

4.5

3 entries

4.8 - 5

Gluconobacter cerinus

348282

4 entries

5.5

Gluconobacter japonicus

pH optimum with 2,6-dichloroindophenol as electron acceptor

760332

Gluconobacter japonicus

two pH optima at pH 4.5 and 7.0 with cytochrome c as electron acceptor

760332

Gluconobacter japonicus

724031

Gluconobacter oxydans

348281

Gluconobacter sp.

pH optimum of free enzyme

760893

4 - 4.5

Gluconobacter japonicus

739849

4 - 4.5

Gluconobacter oxydans

348283

4.5

Gluconobacter japonicus

two pH optima at pH 4.5 and 7.0 with cytochrome c as electron acceptor

760332

4.5

Gluconobacter japonicus

in 50 mM NaAc buffer at 35°C

760337

4.5

Gluconobacter sp.

706060, 762362

Gluconobacter japonicus

740360, 760973

Gluconobacter oxydans

assay at

assay at

pH optimum of enzyme immobilized on nanoporous gold electrodes

760893

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3 - 6

Gluconobacter japonicus

highest activity between pH 3.0 and 6.0 with 2,6-dichloroindophenol as electron acceptor

760332

4.5 - 6.4

Gluconobacter cerinus

pH 4.5: about 85% of maximal activity, pH 6.4: about 50% of maximal activity

348282

4.5 - 6.5

Gluconobacter sp.

enzymatic activity is significantly inhibited at pH lower than 4.5 or higher than 6.5

706060

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Gluconobacter oxydans

348281, 348283

Gluconobacter sp.

temperature optimum of enzyme immobilized on nanoporous gold electrodes

760893

4 entries

Gluconobacter japonicus

739849

Gluconobacter oxydans

assay at

assay at

temperature optimum of free enzyme

760893

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pI VALUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Gluconobacter japonicus

isoelectric focusing

739849

6.6

Gluconobacter japonicus

calculated from amino acid sequence

760337

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show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Gluconobacter cerinus

ASAI IFO 3267

348282

brenda

Gluconobacter frateurii

710853, 762826

brenda

Gluconobacter frateurii CHM 43

762826

brenda

Gluconobacter japonicus

4 entries

Gluconobacter japonicus LMG 1281

761572

brenda

Gluconobacter japonicus NBRC 3260

760976, 761604

brenda

Gluconobacter japonicus NBRC3260

3 entries

Gluconobacter japonicus NBRCA3260

formerly Gluconobacter industrius

739849

brenda

Gluconobacter japonicus NCBR 3260

760332, 760337, 760974

brenda

Gluconobacter oxydans

2 entries

Gluconobacter oxydans 621H

740366

brenda

Gluconobacter sp.

348284, 655081, 686063, 702780, 706060, 723937, 724735, 739767, 740365, 760893, 762362

brenda

Gluconobacter japonicus

739769, 740360, 740361, 740362, 740364, 740368, 760332, 760337, 760656, 760974, 760975, 760976, 760977, 761572, 761604

brenda

Gluconobacter japonicus

formerly Gluconobacter industrius

739849, 740369

brenda

Gluconobacter japonicus

formerly Gluconobacter industrius strain IFO3260

724031

brenda

Gluconobacter japonicus

previously known as Gluconobacter industrius

760973

brenda

Gluconobacter japonicus NBRC3260

740360, 740368, 760975, 760977

brenda

Gluconobacter japonicus NBRC3260

formerly Gluconobacter industrius

740369

brenda

Gluconobacter japonicus NBRC3260

formerly Gluconobacter industrius strain IFO3260

724031

brenda

Gluconobacter oxydans

703276, 740366

brenda

Gluconobacter oxydans

IFO 3260

348281, 348283

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

cytoplasmic membrane

Gluconobacter oxydans

outer surface

348281

brenda

membrane

9 entries

additional information

Gluconobacter cerinus

particulate fraction

348282

brenda

membrane

Gluconobacter frateurii

16020

762826

brenda

membrane

Gluconobacter frateurii CHM 43

16020

brenda

membrane

Gluconobacter japonicus

16020

724031, 739769, 740360, 740362, 740364, 740368, 761604, 760332, 760337, 760656, 760974, 760975

brenda

membrane

Gluconobacter japonicus NBRC 3260

16020

brenda

membrane

Gluconobacter japonicus NBRC3260

16020

brenda

membrane

Gluconobacter japonicus NCBR 3260

16020

brenda

membrane

Gluconobacter oxydans

16020

740366

brenda

membrane

Gluconobacter oxydans 621H

brenda

655081, 724735, 740365

brenda

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

M1VMF7 pBLAST

FDHL_GLUJA

Gluconobacter japonicus

544

59731

Swiss-Prot

Show Sequence

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

138000

Gluconobacter japonicus

760656

140000

4 entries

146400

Gluconobacter japonicus

gel filtration

760332, 760337

16000

Gluconobacter japonicus

1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence

724031

18000

Gluconobacter japonicus

1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE

724031

19700

2 entries

20000

Gluconobacter japonicus

739769, 740368

49000

Gluconobacter japonicus

1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence

724031

50800

2 entries

51000

2 entries

60000

Gluconobacter japonicus

1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence

724031

67000

2 entries

68000

Gluconobacter japonicus

1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE

724031

140000

Gluconobacter oxydans

gel filtration

348281

140000

Gluconobacter sp.

x * 140000

724735

140000

Gluconobacter oxydans

gel filtration

740366

140000

Gluconobacter japonicus

gel filtration

739849, 740362, 761604, 760974

19700

Gluconobacter oxydans

1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE

348281, 348283

19700

Gluconobacter japonicus

739849

50800

Gluconobacter oxydans

1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE

348281, 348283

50800

Gluconobacter japonicus

739849

51000

Gluconobacter japonicus

1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE

724031

51000

Gluconobacter japonicus

739769, 740368

67000

Gluconobacter oxydans

1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE

348281, 348283

67000

Gluconobacter japonicus

739849, 739769, 740368

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Gluconobacter sp.

x * 140000

724735

heterotrimer

20 entries

trimer

Gluconobacter oxydans

1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE

348281, 348283

heterotrimer

Gluconobacter japonicus

1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence

724031

heterotrimer

Gluconobacter japonicus

1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE

724031

heterotrimer

Gluconobacter japonicus

1 * 67000 + 1 * 51000 + 1 * 20000, SDS-PAGE

739769

heterotrimer

Gluconobacter japonicus

1 * 67000 + 1 * 50800 + 1 * 19700, calculated from amino acid sequence

739849

heterotrimer

Gluconobacter japonicus

1 * 67000 + 1 * 51000 + 1 * 20000, SDS-PAGE

740368

heterotrimer

Gluconobacter japonicus

1 * 67000 + 1 * 51000 + 1 * 20000, calculated from amino acid sequence

761604

heterotrimer

Gluconobacter japonicus

760332, 760337

heterotrimer

Gluconobacter japonicus

1 * 67000 + 1 * 51000 + 1 * 20000

760974

heterotrimer

Gluconobacter japonicus

1 * 67000 + 1 * 50000 + 1 * 20000

760975

heterotrimer

Gluconobacter japonicus

1 * 67000 + 1 * 51000 + 1 * 20000, calculated from amino acid sequence

760977, 760976

heterotrimer

Gluconobacter japonicus NBRC 3260

1 * 67000 + 1 * 51000 + 1 * 20000, calculated from amino acid sequence

heterotrimer

Gluconobacter japonicus NBRC3260

1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence

heterotrimer

Gluconobacter japonicus NBRC3260

1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE

heterotrimer

Gluconobacter japonicus NBRC3260

1 * 67000 + 1 * 51000 + 1 * 20000, SDS-PAGE

heterotrimer

Gluconobacter japonicus NBRC3260

1 * 67000 + 1 * 50000 + 1 * 20000

heterotrimer

Gluconobacter japonicus NBRC3260

1 * 67000 + 1 * 51000 + 1 * 20000, calculated from amino acid sequence

heterotrimer

Gluconobacter japonicus NBRCA3260

1 * 67000 + 1 * 50800 + 1 * 19700, calculated from amino acid sequence

heterotrimer

Gluconobacter japonicus NCBR 3260

heterotrimer

Gluconobacter japonicus NCBR 3260

1 * 67000 + 1 * 51000 + 1 * 20000

heterotrimer

Gluconobacter sp.

1 * 67000 + 1 * 50800 + 1 * 19700, calculated from amino acid sequence

760893

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

M450Q

Gluconobacter japonicus

the mutant shows a drastic decrease of the overpotential in the oxidation of D-fructose due to a negative shift in the formal potential of heme 2c as the electron-donating site of the variant

760977

M450Q/DELTA1cFDH

2 entries

M450Q

Gluconobacter japonicus NBRC3260

the mutant shows a drastic decrease of the overpotential in the oxidation of D-fructose due to a negative shift in the formal potential of heme 2c as the electron-donating site of the variant

additional information

4 entries

M450Q/DELTA1cFDH

Gluconobacter japonicus

the direct electron transfer-type bioelectrocatalytic activity of the mutant is higher than that of recombinant native enzyme

761604

M450Q/DELTA1cFDH

Gluconobacter japonicus NBRC 3260

the direct electron transfer-type bioelectrocatalytic activity of the mutant is higher than that of recombinant native enzyme

additional information

Gluconobacter japonicus

an enzyme variant (DELTA1c2cFDH) which lacks 199 amino acid residues including two heme c moieties from N-terminus transports the electrons to the electrode via heme 3c at a more negative potential and at more improved kinetics than the recombinant (native) enzyme

760975

additional information

Gluconobacter japonicus

in mutant M450QDELTA1cFDH, 143 amino acid residues involving heme 1c are removed and M450 as the sixth axial ligand of heme 2c is replaced with glutamine to negatively shift the formal potential of heme 2c

760976

additional information

Gluconobacter japonicus NBRC 3260

additional information

Gluconobacter japonicus NBRC3260

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2.5 - 8.5

Gluconobacter cerinus

immediately inactivated below pH 2.5 and above 8.5

348282

4.5 - 6

Gluconobacter oxydans

stable

348283

5 - 7

Gluconobacter cerinus

1 h, 30°C, stable

348282

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Gluconobacter sp.

the enzyme immobilized onto giant vesicles is stable for at least 20 days at 25°C, while the activity of the free enzyme dropps to about 20% of its initial activity during the same period of time

655081

Gluconobacter oxydans

rapid inactivation beyond

348283

Gluconobacter cerinus

10 min, no loss of activity

348282

Gluconobacter cerinus

10 min, 15% loss of activity

348282

Gluconobacter cerinus

10 min, complete loss of activity

348282

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

D-fructose, 5-10%, stabilizes

Gluconobacter oxydans

348281

detergents stabilize

Gluconobacter oxydans

348283

glycerol, 5-10%, stabilizes

Gluconobacter oxydans

348281

sucrose, 5-10%, stabilizes

Gluconobacter oxydans

348281

the enzyme immobilized onto giant vesicles is stable for at least 20 days at 25°C, while the activity of the free enzyme dropps to about 20% of its initial activity during the same period of time

Gluconobacter sp.

655081

Triton X-100 is essential for preservation of purified enzyme

Gluconobacter oxydans

348281

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C or below, activity completely preserved

Gluconobacter oxydans

348281, 348283

0-4°C, pH 4.0-5.0, 0.1% Triton X-100, 1 mM 2-mercaptoethanol, for at least 2 weeks

Gluconobacter oxydans

348281

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

2 entries

ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Toyopearl CM-650 column chromatography, and Superdex 200 gel filtration

Gluconobacter japonicus

740368

DEAE-Sepharose column chromatography

Gluconobacter japonicus

724031

Gluconobacter cerinus

348282

Gluconobacter oxydans

348281

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Gluconobacter japonicus strain LMG 26773 and Gluconobacter oxydans

Gluconobacter japonicus

761572

expressed in Gluconobacter oxydans strain NBRC12528

Gluconobacter japonicus

740368

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

analysis

Gluconobacter oxydans

the enzyme is a satisfactory reagent for microdetermination of D-fructose

348283

biofuel production

Gluconobacter sp.

an enzymatic gold bioanode fabricated with fructose dehydrogenase and a polyaniline film can be used as a single-compartment fructose biofuel cell

biotechnology

food industry

industry

direct electron-transfer bioelectrocatalysis can be used in biosensors, biofuel cells and bioreactors, FDH immobilised on Ketjen black electroconductive material produces a catalytic oxidation wave of D-fructose without a mediator, the electron in FDH seems to be directly transferred to the electrode via the heme c site

686063

synthesis

2 entries

biotechnology

Gluconobacter sp.

multi-walled carbon nanotubes synthesized on platinum plate (MWCNTs/Pt) electrode are immediately immersed into solutions of FDH to immobilize the enzyme onto electrode surfaces. Thereafter, a well-defined catalytic oxidation current based on FDH is observed from ca. -0.15V, which is close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current versus substrate, the calibration range for the fructose concentration is up to ca. 40 mmol/dm3, and the apparent Michaelis-Menten constant is evaluated to be 11 mmol/l. The obtained results are useful in applications to prepare the third-generation biosensors and other future bioelectrochemical devices

702780

biotechnology

Gluconobacter oxydans

an automated, enzymatic insulin assay is developed. Principle: Fructose is produced by the action of inulinase on inulin present in the sample. The resulting fructose reacts with D-fructose dehydrogenase in the presence of the oxidized form of 1-methoxy-5-methylphenazinium methylsulfate (1-m-PMS) to produce the reduced form of 1-m-PMS. Reduced 1-m-PMS acts on dissolved oxygen to produce hydrogen peroxide, which, through the action of peroxidase, oxidatively condenses N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine and 4-aminoantipyrine to transform them into quinoneimine dye. The absorbance of the quinoneimine dye is measured spectrophotometrically to determine the concentration of inulin in the sample. The new enzymatic assay offers a more convenient and more accurate measurement of inulin and may be suitable for routine procedures by automated analyzers in clinical laboratories

703276

biotechnology

Gluconobacter sp.

using fructose dehydrogenase-catalyzed conversion of d-fructose to 5-ketofructose, followed by quantitation of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] formazan production by direct spectrophotometry, an assay to measure serum fructose concentration is developed. The fructose dehydrogenase-based enzymatic assay correlates highly with gas chromatography-mass spectroscopic analysis of serum fructose. The assay is highly specific, exhibits no cross-reactivity with other sugars and is easy to perform

706060

food industry

Gluconobacter japonicus

the enzyme can be used as biosensor to quantify D-fructose in commercial beverages and honey

761604

food industry

Gluconobacter japonicus NBRC 3260

the enzyme can be used as biosensor to quantify D-fructose in commercial beverages and honey

synthesis

Gluconobacter frateurii

Gluconobacter frateurii CHM 43 have D-mannitol dehydrogenase (quinoprotein glycerol dehydrogenase) and flavoprotein D-fructose dehydrogenase in the membranes. When the two enzymes are functional, D-mannitol is converted to 5-keto-D-fructose with 65% yield when cultivated on D-mannitol. 5-Keto-D-fructose production with almost 100% yield is realized with the resting cells

762826

synthesis

Gluconobacter frateurii CHM 43

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

348281

Ameyama, M.; Adachi, O.

D-Fructose dehydrogenase from Gluconobacter industrius, membrane-bound

Methods Enzymol.

154-159

1982

Gluconobacter oxydans

brenda

348282

Yamada, Y.; Aida, K.; Uemura, T.

Enzymatic studies on the oxidation of sugar and sugar alcohol. I. Purification and properties of particle-bound fructose dehydrogenase

J. Biochem.

636-646

1967

Gluconobacter cerinus

6059959

brenda

348283

Ameyama, M.; Shinagawa, E.; Matsushita, K.; Adachi, O.

D-Fructose dehydrogenase of Gluconobacter industrius: purification, characterization, and application to enzymatic microdetermination of D-fructose

J. Bacteriol.

145

814-823

1981

Gluconobacter oxydans

7462161

brenda

348284

Marcinkeviciene, J.; Johansson, G.

Kinetic studies of the active sites functioning in the quinohemoprotein fructose dehydrogenase

FEBS Lett.

318

23-26

1993

Gluconobacter sp.

8436220

brenda

655081

Kato, K.; Walde, P.; Mitsui, H.; Higashi, N.

Enzymatic activity and stability of D-fructose dehydrogenase and sarcosine dehydrogenase immobilized onto giant vesicles

Biotechnol. Bioeng.

415-423

2003

Gluconobacter sp.

14574698

brenda

686063

Kamitaka, Y.; Tsujimura, S.; Kano, K.

High current density bioelectrolysis of D-fructose at fructose dehydrogenase-adsorbed and Ketjen black-modified electrodes without a mediator

Chem. Lett.

218-219

2007

Gluconobacter sp.

brenda

702780

Tominaga, M.; Nomura, S.; Taniguchi, I.

D-fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode

Biosens. Bioelectron.

1184-1188

2009

Gluconobacter sp.

18707862

brenda

703276

Kimata, S.; Mizuguchi, K.; Hattori, S.; Teshima, S.; Orita, Y.

Evaluation of a new automated, enzymatic inulin assay using D-fructose dehydrogenase

Clin. Exp. Nephrol.

341-349

2009

Gluconobacter oxydans

19365704

brenda

706060

Hui, H.; Huang, D.; McArthur, D.; Nissen, N.; Boros, L.G.; Heaney, A.P.

Direct spectrophotometric determination of serum fructose in pancreatic cancer patients

Pancreas

706-712

2009

Gluconobacter sp.

19506535

brenda

710853

Tsujimura, S.; Nishina, A.; Kamitaka, Y.; Kano, K.

Coulometric D-fructose biosensor based on direct electron transfer using D-fructose dehydrogenase

Anal. Chem.

9383-9387

2009

Gluconobacter frateurii

19908905

brenda

723937

Sasaki, Y.; Sugihara, T.; Osakai, T.

Electron transfer mediated by membrane-bound D-fructose dehydrogenase adsorbed at an oil/water interface

Anal. Biochem.

417

129-135

2011

Gluconobacter sp.

21708120

brenda

724031

Kawai, S.; Goda-Tsutsumi, M.; Yakushi, T.; Kano, K.; Matsushita, K.

Heterologous overexpression and characterization of a flavoprotein-cytochrome c complex fructose dehydrogenase of Gluconobacter japonicus NBRC3260

Appl. Environ. Microbiol.

1654-1660

2013

Gluconobacter japonicus, Gluconobacter japonicus NBRC3260

23275508

brenda

724735

Sasakura, K.; Shirai, O.; Hichiri, K.; Goda-Tsutsumi, M.; Tsujimura, S.; Kano, K.

Ion transport across planar bilayer lipid membrane driven by D-fructose dehydrogenase-catalyzed electron transport

Chem. Lett.

486-488

2011

Gluconobacter sp.

brenda

739767

Hickey, D.; Giroud, F.; Schmidtke, D.; Glatzhofer, D.; Minteer, S.

Enzyme cascade for catalyzing sucrose oxidation in a biofuel cell

ACS Catal.

2729-2737

2013

Gluconobacter sp.

brenda

739769

Sarauli, D.; Wettstein, C.; Peters, K.; Schulz, B.; Fattakhova-Rohlfing, D.; Lisdat, F.

Interaction of fructose dehydrogenase with a sulfonated polyaniline: application for enhanced bioelectrocatalysis

ACS Catal.

2081-2087

2015

Gluconobacter japonicus

brenda

739849

Wettstein, C.; Kano, K.; Sch�fer, D.; Wollenberger, U.; Lisdat, F.

Interaction of flavin-dependent fructose dehydrogenase with cytochrome c as basis for the construction of biomacromolecular architectures on electrodes

Anal. Chem.

6382-6389

2016

Gluconobacter japonicus, Gluconobacter japonicus NBRCA3260

27213223

brenda

740360

Kawai, S.; Yakushi, T.; Matsushita, K.; Kitazumi, Y.; Shirai, O.; Kano, K.

The electron transfer pathway in direct electrochemical communication of fructose dehydrogenase with electrodes

Electrochem. Commun.

28-31

2014

Gluconobacter japonicus, Gluconobacter japonicus NBRC3260

brenda

740361

Funabashi, H.; Murata, K.; Tsujimura, S.

Effect of pore size of MgO-templated carbon on the direct electrochemistry of D-fructose dehydrogenase

Electrochemistry

372-375

2015

Gluconobacter japonicus

brenda

740362

Hichiri, K.; Shirai, O.; Kitazumi, Y.; Kano, K.

Coupling of proton transport across planar lipid bilayer and electron transport catalyzed by membrane-bound enzyme D-fructose dehydrogenase

Electrochemistry

328-333

2016

Gluconobacter japonicus

brenda

740364

Kawai, S.; Yakushi, T.; Matsushita, K.; Kitazumi, Y.; Shirai, O.; Kano, K.

Role of a non-ionic surfactant in direct electron transfer-type bioelectrocatalysis by fructose dehydrogenase

Electrochim. Acta

152

19-24

2015

Gluconobacter japonicus

brenda

740365

Sugimoto, Y.; Kitazumi, Y.; Shirai, O.; Yamamoto, M.; Kano, K.

Role of 2-mercaptoethanol in direct electron transfer-type bioelectrocatalysis of fructose dehydrogenase at Au electrodes

Electrochim. Acta

170

242-247

2015

Gluconobacter sp.

brenda

740366

Sugimoto, Y.; Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.

Function of C-terminal hydrophobic region in fructose dehydrogenase

Electrochim. Acta

176

976-981

2015

Gluconobacter oxydans, Gluconobacter oxydans 621H

brenda

740368

Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.

Bioelectrochemical characterization of the reconstruction of heterotrimeric fructose dehydrogenase from its subunits

Electrochim. Acta

210

689-694

2016

Gluconobacter japonicus, Gluconobacter japonicus NBRC3260

brenda

740369

Xia, H.; Hibino, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.

Interaction between D-fructose dehydrogenase and methoxy-substituent-functionalized carbon surface to increase productive orientations

Electrochim. Acta

218

41-46

2016

Gluconobacter japonicus, Gluconobacter japonicus NBRC3260

brenda

760332

Bollella, P.; Hibino, Y.; Kano, K.; Gorton, L.; Antiochia, R.

The influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase

Anal. Bioanal. Chem.

410

3253-3264

2018

Gluconobacter japonicus, Gluconobacter japonicus NCBR 3260

29564502

brenda

760337

Bollella, P.; Hibino, Y.; Kano, K.; Gorton, L.; Antiochia, R.

Highly sensitive membraneless fructose biosensor based on fructose dehydrogenase immobilized onto aryl thiol modified highly porous gold electrode characterization and application in food samples

Anal. Chem.

12131-12136

2018

Gluconobacter japonicus, Gluconobacter japonicus NCBR 3260

30148350

brenda

760656

Adachi, T.; Kaida, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.

Bioelectrocatalytic performance of D-fructose dehydrogenase

Bioelectrochemistry

129

1-9

2019

Gluconobacter japonicus

31063949

brenda

760893

Siepenkoetter, T.; Salaj-Kosla, U.; Magner, E.

The immobilization of fructose dehydrogenase on nanoporous gold electrodes for the detection of fructose

ChemElectroChem

905-912

2017

Gluconobacter sp.

brenda

760973

Voitechovic, E.; Vektariene, A.; Vektaris, G.; Janciene, R.; Razumiene, J.; Gureviciene, V.

1,4-Benzoquinone derivatives for enhanced bioelectrocatalysis by fructose dehydrogenase from Gluconobacter japonicus Towards promising D-fructose biosensor development

Electroanalysis

1005-1016

2020

Gluconobacter japonicus

brenda

760974

Hibino, Y.; Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.

Construction of a protein-engineered variant of D-fructose dehydrogenase for direct electron transfer-type bioelectrocatalysis

Electrochem. Commun.

112-115

2017

Gluconobacter japonicus, Gluconobacter japonicus NCBR 3260

brenda

760975

Kaida, Y.; Hibino, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.

Ultimate downsizing of D-fructose dehydrogenase for improving the performance of direct electron transfer-type bioelectrocatalysis

Electrochem. Commun.

101-105

2019

Gluconobacter japonicus, Gluconobacter japonicus NBRC3260

brenda

760976

Hibino, Y.; Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.

Protein-engineering improvement of direct electron transfer-type bioelectrocatalytic properties of D-fructose dehydrogenase

Electrochemistry

47-51

2019

Gluconobacter japonicus, Gluconobacter japonicus NBRC 3260

brenda

760977

Kaida, Y.; Hibino, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.

Discussion on direct electron transfer-type bioelectrocatalysis of downsized and axial-ligand exchanged variants of D-fructose dehydrogenase

Electrochemistry

195-199

2020

Gluconobacter japonicus, Gluconobacter japonicus NBRC3260

brenda

761572

Hoffmann, J.; Hoevels, M.; Kosciow, K.; Deppenmeier, U.

Synthesis of the alternative sweetener 5-ketofructose from sucrose by fructose dehydrogenase and invertase producing Gluconobacter strains

J. Biotechnol.

307

164-174

2020

Gluconobacter japonicus, Gluconobacter japonicus LMG 1281

31704125

brenda

761604

Suzuki, Y.; Kano, K.; Shirai, O.; Kitazumi, Y.

Diffusion-limited electrochemical D-fructose sensor based on direct electron transfer-type bioelectrocatalysis by a variant of D-fructose dehydrogenase at a porous gold microelectrode

J. Electroanal. Chem.

877

114651

2020

Gluconobacter japonicus, Gluconobacter japonicus NBRC 3260

brenda

762362

Kuwahara, T.; Kameda, M.; Isozaki, K.; Toriyama, K.; Kondo, M.; Shimomura, M.

Bioelectrocatalytic fructose oxidation with fructose dehydrogenase-bearing conducting polymer films for biofuel cell application

React. Funct. Polym.

116

87-91

2017

Gluconobacter sp.

brenda

762826

Adachi, O.; Nguyen, T.M.; Hours, R.A.; Kataoka, N.; Matsushita, K.; Akakabe, Y.; Yakushi, T.

5-Keto-D-fructose production from sugar alcohol by isolated wild strain Gluconobacter frateurii CHM 43

Biosci. Biotechnol. Biochem.

1745-1747

2020

Gluconobacter frateurii, Gluconobacter frateurii CHM 43

32427050

brenda

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