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(R)-lactate + 2 ferricytochrome c
pyruvate + 2 ferrocytochrome c
quinone is the preferred electron acceptor of the enzyme
-
-
?
(R)-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pyruvate + ?
quinone is the preferred electron acceptor of the enzyme
-
-
?
(R)-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
-
-
?
(R)-lactate + a quinone
pyruvate + a quinol
-
-
-
-
?
(R)-lactate + coenzyme Q10
pyruvate + reduced coenzyme Q10
(R)-lactate + cytochrome c
pyruvate + reduced cytochrome c
-
-
-
?
(R)-lactate + oxidized phenazine methosulfate
pyruvate + reduced phenazine methosulfate
(R)-lactate + phenazine methosulfate
pyruvate + reduced phenazine methosulfate
-
-
-
-
?
(R,S)-2-hydroxybutanoate + a quinone
2-oxobutanoate + a quinol
-
12% of the activity with (R)-lactate
-
-
?
(S)-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pyruvate + ?
quinone is the preferred electron acceptor of the enzyme
-
-
?
(S)-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
poor substrate
-
-
?
(S)-lactate + oxidized dichlorophenolindophenol
pyruvate + reduced dichlorophenolindophenol
-
14% of the activity with (R)-lactate
-
-
?
D-2-hydroxybutanoate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
2-oxobutanoate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
-
-
?
D-2-hydroxybutyrate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
2-oxobutanoate + ?
-
-
-
?
D-2-hydroxybutyrate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
? + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
-
-
?
D-2-hydroxybutyrate + ubiquinone-10
? + ubiquinol-10
-
-
-
?
D-glycerate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
?
-
-
-
?
D-glycerate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
? + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
-
-
?
D-glycerate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
D-glyceraldehyde + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
poor substrate
-
-
?
D-glycerate + ubiquinone-10
? + ubiquinol-10
-
-
-
?
D-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
D-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
-
-
?
D-lactate + ferricyanide
pyruvate + ferrocyanide
D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
D-lactate + ubiquinone 1
pyruvate + reduced ubiquinone 1
D-lactate + ubiquinone-10
pyruvate + ubiquinol-10
-
-
-
?
DL-2-hydroxybutanoate + 2,6-dichloroindophenol
2-oxobutanoate + reduced 2,6-dichloroindophenol
L-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
L-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
-
-
?
L-lactate + ubiquinone-10
pyruvate + ubiquinol-10
-
-
-
?
additional information
?
-
(R)-lactate + coenzyme Q10
pyruvate + reduced coenzyme Q10
quinone is the preferred electron acceptor of the enzyme
-
-
?
(R)-lactate + coenzyme Q10
pyruvate + reduced coenzyme Q10
-
-
-
?
(R)-lactate + oxidized phenazine methosulfate
pyruvate + reduced phenazine methosulfate
-
-
-
-
?
(R)-lactate + oxidized phenazine methosulfate
pyruvate + reduced phenazine methosulfate
-
-
-
-
?
D-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-lactate + ferricyanide
pyruvate + ferrocyanide
-
-
-
-
?
D-lactate + ferricyanide
pyruvate + ferrocyanide
-
-
-
-
?
D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
-
-
-
?
D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
-
-
-
?
D-lactate + ubiquinone 1
pyruvate + reduced ubiquinone 1
-
-
-
-
?
D-lactate + ubiquinone 1
pyruvate + reduced ubiquinone 1
-
-
-
-
?
DL-2-hydroxybutanoate + 2,6-dichloroindophenol
2-oxobutanoate + reduced 2,6-dichloroindophenol
-
about 5% of the activity with D-lactate
-
-
?
DL-2-hydroxybutanoate + 2,6-dichloroindophenol
2-oxobutanoate + reduced 2,6-dichloroindophenol
-
about 5% of the activity with D-lactate
-
-
?
L-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
about 5% of the activity with D-lactate
-
-
?
L-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
about 5% of the activity with D-lactate
-
-
?
L-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
-
?
L-lactate + 2,6-dichloroindophenol
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
-
?
additional information
?
-
-
no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate
-
-
?
additional information
?
-
-
no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate
-
-
?
additional information
?
-
-
not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate
-
-
?
additional information
?
-
-
not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate
-
-
?
additional information
?
-
the enzyme exhibits narrow substrate specificity. No activity with D-2-hydroxybutyrate, L-2-hydroxybutyrate, D-mandelate, L-mandelate, D-3-phenyllactate, D-glycerate, L-glycerate, DL-2-hydroxyisocaproate, and DL-2-hydroxyoctanoate, with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as an electron acceptor
-
-
-
additional information
?
-
quinone is the preferred electron acceptor of the enzyme. No substrates: glycolate, L-2-hydroxybutyrate, D-mandelate, L-mandelate, D-3-phenyllactate, L-glycerate, DL-2-hydroxyisocaproate, and DL-2-hydroxyoctanoate
-
-
-
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1.2
(R,S)-2-hydroxybutanoate
-
pH 8.0, 23°C
0.031 - 0.048
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
0.0041
coenzyme Q10
cosubstrate (R)-lactate, pH 7.4, 30°C
0.0095 - 0.0515
cytochrome c
0.1 - 0.17
D-2-hydroxybutanoate
0.0041
Ubiquinone-10
pH 7.4, 30°C
0.057
(R)-lactate
pH 7.4. 30°C
0.057
(R)-lactate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
0.094
(R)-lactate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
0.4
(R)-lactate
-
pH 8.0, 23°C
0.4
(R)-lactate
-
presence of Triton X-100, pH 8.0, 23°C
0.6
(R)-lactate
-
pH 8.0, 23°C
0.6
(R)-lactate
-
mutant F39W, pH 7.2, 20°C
0.9
(R)-lactate
-
mutant F176W, pH 7.2, 20°C
0.9
(R)-lactate
-
mutant F340W, pH 7.2, 20°C
1.1
(R)-lactate
-
mutant F435W, pH 7.2, 20°C
1.4
(R)-lactate
-
presence of cyanide, pH 8.0, 23°C
1.5
(R)-lactate
-
mutant Y243W, pH 7.2, 20°C
1.5
(R)-lactate
-
mutant Y388W, pH 7.2, 20°C
1.6
(R)-lactate
-
wild-type, pH 7.2, 20°C
1.8
(R)-lactate
-
mutant L517W, pH 7.2, 20°C
2.1
(R)-lactate
-
mutant F12W, pH 7.2, 20°C
2.3
(R)-lactate
-
mutant F361W, pH 7.2, 20°C
1.04
(S)-lactate
pH 7.4. 30°C
1.04
(S)-lactate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
1.78
(S)-lactate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
18
(S)-lactate
-
pH 8.0, 23°C
0.031
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
cosubstrate (R)-lactate, isolated FAD-containing dehydrogenase domain, pH 7.4, 30°C
0.048
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pH 7.4, 30°C
0.048
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
cosubstrate (R)-lactate, pH 7.4, 30°C
0.0095
cytochrome c
cosubstrate (R)-lactate, isolated FAD-containing dehydrogenase domain, pH 7.4, 30°C
0.0515
cytochrome c
cosubstrate (R)-lactate, pH 7.4, 30°C
0.1
D-2-hydroxybutanoate
pH 7.4. 30°C
0.17
D-2-hydroxybutanoate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
0.1
D-2-hydroxybutyrate
pH 7.4, 30°C
0.1
D-2-hydroxybutyrate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
1.38
D-glycerate
pH 7.4, 30°C
1.38
D-glycerate
pH 7.4. 30°C
1.38
D-glycerate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
1.67
D-glycerate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
0.057
D-lactate
pH 7.4, 30°C
0.5
D-lactate
-
presence of Triton X-100, pH 7.8, 25°C
0.61
D-lactate
-
pH 7.5, 30°C
1.4
D-lactate
-
pH 7.8, 25°C
1.04
L-lactate
pH 7.4, 30°C
21
L-lactate
-
presence of Triton X-100, pH 7.8, 25°C
30
L-lactate
-
pH 7.8, 25°C
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5.3 - 128
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
2.4
coenzyme Q10
cosubstrate (R)-lactate, pH 7.4, 30°C
13 - 66
D-2-hydroxybutanoate
120
D-lactate
pH 7.4, 30°C
9.5
L-lactate
pH 7.4, 30°C
2.4
Ubiquinone-10
pH 7.4, 30°C
17
(R)-lactate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
120
(R)-lactate
pH 7.4. 30°C
120
(R)-lactate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
1.5
(S)-lactate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
9.5
(S)-lactate
pH 7.4. 30°C
9.5
(S)-lactate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
5.3
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
cosubstrate (R)-lactate, isolated FAD-containing dehydrogenase domain, pH 7.4, 30°C
128
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pH 7.4, 30°C
128
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
cosubstrate (R)-lactate, pH 7.4, 30°C
0.49
cytochrome c
cosubstrate (R)-lactate, pH 7.4, 30°C
2
cytochrome c
cosubstrate (R)-lactate, isolated FAD-containing dehydrogenase domain, pH 7.4, 30°C
13
D-2-hydroxybutanoate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
66
D-2-hydroxybutanoate
pH 7.4. 30°C
66
D-2-hydroxybutyrate
pH 7.4, 30°C
66
D-2-hydroxybutyrate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
0.78
D-glycerate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
8
D-glycerate
pH 7.4, 30°C
8
D-glycerate
pH 7.4. 30°C
8
D-glycerate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
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2.7 - 2700
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
590
coenzyme Q10
cosubstrate (R)-lactate, pH 7.4, 30°C
78 - 660
D-2-hydroxybutanoate
2100
D-lactate
pH 7.4, 30°C
0.59
Ubiquinone-10
pH 7.4, 30°C
176
(R)-lactate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
2100
(R)-lactate
pH 7.4. 30°C
2100
(R)-lactate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
0.84
(S)-lactate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
9
(S)-lactate
pH 7.4. 30°C
9
(S)-lactate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
2.7
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
pH 7.4, 30°C
170
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
cosubstrate (R)-lactate, isolated FAD-containing dehydrogenase domain, pH 7.4, 30°C
2700
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
cosubstrate (R)-lactate, pH 7.4, 30°C
9.5
cytochrome c
cosubstrate (R)-lactate, pH 7.4, 30°C
210
cytochrome c
cosubstrate (R)-lactate, isolated FAD-containing dehydrogenase domain, pH 7.4, 30°C
78
D-2-hydroxybutanoate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
660
D-2-hydroxybutanoate
pH 7.4. 30°C
660
D-2-hydroxybutyrate
pH 7.4, 30°C
660
D-2-hydroxybutyrate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
0.47
D-glycerate
isolated FAD-containing dehydrogenase domain, pH 7.4. 30°C
6
D-glycerate
pH 7.4, 30°C
6
D-glycerate
pH 7.4. 30°C
6
D-glycerate
pH 7.4, 30°C, acceptor: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
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Kato, O.; Youn, J.; Stansen, K.; Matsui, D.; Oikawa, T.; Wendisch, V.
Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate
BMC Microbiol.
10
321
2010
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
brenda
Futai, M.
Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties
Biochemistry
12
2468-2474
1973
Escherichia coli
brenda
Pratt, E.; Fung, L.; Flower, J.; Ho, C.
Membrane-bound D-lactate dehydrogenase from Escherichia coli: Purification and properties
Biochemistry
18
312-316
1979
Escherichia coli, Escherichia coli W3110trpA33
brenda
Matsushita, K., Kaback, H.R.
D-Lactate oxidation and generation of the proton electrochemical gradient in membrane vesicles from Escherichia coli GR19N and in proteoliposomes reconstituted with purified D-lactate dehydrogenase and cytochrome O oxidase
Biochemistry
25
2321-2327
1986
Escherichia coli, Escherichia coli GR19N
brenda
Peersen, O.; Pratt, E.; Truong, H.; Ho, C.; Rule, G.
Site-specific incorporation of 5-fluorotryptophan as a probe of the structure and function of the membrane-bound D-lactate dehydrogenase of Escherichia coli: A19F nuclear magnetic resonance study
Biochemistry
29
3256-3262
1990
Escherichia coli
brenda
Kohn, L.; Kaback, H.
Mechanisms of active transport in isolated bacterial membrane vesicles
J. Biol. Chem.
248
7012-7017
1973
Escherichia coli, Escherichia coli ML 308-225
brenda
Jiang, T.; Guo, X.; Yan, J.; Zhang, Y.; Wang, Y.; Zhang, M.; Sheng, B.; Ma, C.; Xu, P.; Gao, C.
A bacterial multidomain NAD-independent D-lactate dehydrogenase utilizes flavin adenine dinucleotide and Fe-S clusters as cofactors and quinone as an electron acceptor for D-lactate oxidization
J. Bacteriol.
199
e00342
2017
Pseudomonas putida KT2440 (Q88DT2)
brenda
Jiang, T.; Guo, X.; Yan, J.; Zhang, Y.; Wang, Y.; Zhang, M.; Sheng, B.; Ma, C.; Xu, P.; Gao, C.
A bacterial multidomain NAD-independent D-lactate dehydrogenase utilizes flavin adenine dinucleotide and Fe-S clusters as cofactors and quinone as an electron acceptor for D-lactate oxidization
J. Bacteriol.
199
e00342-17
2017
Pseudomonas putida (Q88DT2)
brenda