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IUBMB Comments The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation.
Synonyms isoH ,
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4-hydroxy-3-glutathionyl-3-methylbut-1-ene dehydrogenase
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2-(glutathion-S-yl)-2-methylbut-3-en-1-ol + 2 NAD+ + H2O = 2-(glutathion-S-yl)-2-methylbut-3-enoate + 2 NADH + 2 H+
overall reaction
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2-(glutathion-S-yl)-2-methylbut-3-en-1-ol + NAD+ = 2-(glutathion-S-yl)-2-methylbut-3-enal + NADH + H+
(1a)
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2-(glutathion-S-yl)-2-methylbut-3-enal + NAD+ + H2O = 2-(glutathion-S-yl)-2-methylbut-3-enoate + NADH + H+
(1b)
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2-(glutathione-S-yl)-2-methylbut-3-en-1-ol:NAD+ oxidoreductase
The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation.
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1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+
N-(2-methyl-1-oxobut-3-en-2-yl)-L-gamma-glutamyl-L-cysteinylglycine + NADH + H+
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Substrates: -
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2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O
2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+
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Substrates: -
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additional information
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Substrates: no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates
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1.4
1-hydroxy-2-glutathionyl-2-methyl-3-butene
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at pH 10.0 and 30°C
0.07
NAD+
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at pH 10.0 and 30°C
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18
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after 6.2fold purification, at pH 10.0 and 30°C
2.9
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crude extract, at pH 10.0 and 30°C
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ISOH_RHOSX
Rhodococcus sp. (strain AD45)
226
0
24062
Swiss-Prot
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25000
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2 * 25000, SDS-PAGE
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homodimer
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2 * 25000, SDS-PAGE
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Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration
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Van Hylckama Vlieg, J.; Kingma, J.; Kruizinga, W.; Janssen, D.
Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45
J. Bacteriol.
181
2094-2101
1999
Rhodococcus sp. AD45
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