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3alpha-hydroxy-bile-acid-CoA-ester:NAD+ 3-oxidoreductase
This bacterial enzyme is involved in the 7-dehydroxylation process associated with bile acid degradation. The enzyme has very little activity with unconjugated bile acid substrates. It has similar activity with choloyl-CoA, chenodeoxycholoyl-CoA, deoxycholoyl-CoA, and lithocholoyl-CoA.
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3-oxochenodeoxycholoyl-CoA + NAD+
? + NADH + H+
3-oxocholoyl CoA + NAD+
? + NADH + H+
3-oxodeoxycholoyl-CoA + NAD+
? + NADH + H+
3-oxolithocholoyl-CoA + NAD+
? + NADH + H+
3alpha-hydroxy bile acid CoA ester + NAD+
3-oxo bile acid CoA ester + NADH + H+
chenodeoxycholic acid + NAD+
7-alpha-hydroxy-3-oxo-5beta-cholanate + NADH + H+
chenodeoxycholoyl-CoA + NADP+
3-oxochenodeoxycholoyl-CoA + NADPH + H+
Substrates: -
Products: -
?
chenodeoxycholyl CoA + NAD+
3-oxo-chenodeoxycholy-CoA + NADH + H+
Substrates: -
Products: -
?
cholic acid + NAD+
3-oxocholic acid + NADH + H+
Substrates: -
Products: -
?
cholic acid + NAD+
? + NADH + H+
-
Substrates: -
Products: -
?
choloyl-CoA + NAD+
3-oxocholoyl-CoA + NADH + H+
deoxycholoyl-CoA + NAD+
3-oxodeoxycholoyl-CoA + NADH + H+
Substrates: -
Products: -
?
glycocholic acid + NAD+
N-[7alpha,12-alpha-3,24-dioxo-5beta-cholan-24-yl]glycine + NADH + H+
Substrates: -
Products: -
?
lithocholoyl-CoA + NAD+
3-oxolithocholoyl-CoA + NADH + H+
Substrates: -
Products: -
?
taurocholic acid + NAD+
3-oxotaurocholic acid + NADH + H+
Substrates: -
Products: -
?
3-oxochenodeoxycholoyl-CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-oxochenodeoxycholoyl-CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-oxocholoyl CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-oxocholoyl CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-oxodeoxycholoyl-CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-oxodeoxycholoyl-CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-oxolithocholoyl-CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-oxolithocholoyl-CoA + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3alpha-hydroxy bile acid CoA ester + NAD+
3-oxo bile acid CoA ester + NADH + H+
-
Substrates: -
Products: -
?
3alpha-hydroxy bile acid CoA ester + NAD+
3-oxo bile acid CoA ester + NADH + H+
-
Substrates: -
Products: -
?
chenodeoxycholic acid + NAD+
7-alpha-hydroxy-3-oxo-5beta-cholanate + NADH + H+
Substrates: -
Products: -
?
chenodeoxycholic acid + NAD+
7-alpha-hydroxy-3-oxo-5beta-cholanate + NADH + H+
Substrates: -
Products: -
?
choloyl-CoA + NAD+
3-oxocholoyl-CoA + NADH + H+
-
Substrates: preferred substrate
Products: -
?
choloyl-CoA + NAD+
3-oxocholoyl-CoA + NADH + H+
Substrates: -
Products: -
?
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3alpha-hydroxy bile acid CoA ester + NAD+
3-oxo bile acid CoA ester + NADH + H+
3alpha-hydroxy bile acid CoA ester + NAD+
3-oxo bile acid CoA ester + NADH + H+
-
Substrates: -
Products: -
?
3alpha-hydroxy bile acid CoA ester + NAD+
3-oxo bile acid CoA ester + NADH + H+
-
Substrates: -
Products: -
?
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0.0011 - 0.0033
3-oxochenodeoxycholoyl-CoA
0.0018 - 0.0091
3-oxocholoyl-CoA
0.0015 - 0.0056
3-oxodeoxycholoyl-CoA
0.0013 - 0.0083
3-oxolithocholoyl-CoA
0.334 - 0.555
chenodeoxycholic acid
0.023 - 0.053
chenodeoxycholoyl-CoA
0.802 - 1.158
cholic acid
0.032 - 0.106
choloyl-CoA
0.016 - 0.017
deoxycholoyl-CoA
0.205 - 0.383
glycocholic acid
0.018 - 0.022
lithocholoyl-CoA
0.201 - 0.329
taurocholic acid
0.0011
3-oxochenodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.0033
3-oxochenodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.0018
3-oxocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.0091
3-oxocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.0015
3-oxodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.0056
3-oxodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.0013
3-oxolithocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.0083
3-oxolithocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.334
chenodeoxycholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.555
chenodeoxycholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.023
chenodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.053
chenodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.802
cholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
1.158
cholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.032
choloyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.106
choloyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.016
deoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.017
deoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.205
glycocholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.383
glycocholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.018
lithocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.022
lithocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.0067
NAD+
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.017
NAD+
mutant E42A of isoform BaiA2, at pH 8.7 and 37°C
0.017
NAD+
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.015
NADP+
mutant E42A of isoform BaiA2, at pH 8.7 and 37°C
0.084
NADP+
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.883
NADP+
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.201
taurocholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.329
taurocholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
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0.17 - 0.4
3-oxochenodeoxycholoyl-CoA
0.14 - 0.55
3-oxocholoyl-CoA
0.15 - 0.73
3-oxodeoxycholoyl-CoA
0.12 - 0.7
3-oxolithocholoyl-CoA
0.055 - 0.25
chenodeoxycholic acid
0.2 - 0.82
chenodeoxycholoyl-CoA
0.16 - 0.87
deoxycholoyl-CoA
0.145 - 0.42
glycocholic acid
0.18 - 0.73
lithocholoyl-CoA
0.15 - 0.4
taurocholic acid
0.17
3-oxochenodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.4
3-oxochenodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.14
3-oxocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.55
3-oxocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.15
3-oxodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.73
3-oxodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.12
3-oxolithocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.7
3-oxolithocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.055
chenodeoxycholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.25
chenodeoxycholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.2
chenodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.82
chenodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.09
cholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.27
cholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.17
choloyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
1.23
choloyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.16
deoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.87
deoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.145
glycocholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.42
glycocholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.18
lithocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.73
lithocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.089
NAD+
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.3
NAD+
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.32
NAD+
mutant E42A of isoform BaiA2, at pH 8.7 and 37°C
0.025
NADP+
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.05
NADP+
mutantE42A of isoform BaiA2, at pH 8.7 and 37°C
0.11
NADP+
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.15
taurocholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.4
taurocholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
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120.5 - 151.5
3-oxochenodeoxycholoyl-CoA
60.4 - 76.85
3-oxocholoyl-CoA
101.7 - 131
3-oxodeoxycholoyl-CoA
84.3 - 93.58
3-oxolithocholoyl-CoA
0.17 - 0.45
chenodeoxycholic acid
8.7 - 15.4
chenodeoxycholoyl-CoA
9.32 - 54.2
deoxycholoyl-CoA
0.72 - 1
glycocholic acid
10.18 - 33.3
lithocholoyl-CoA
0.73 - 1
taurocholic acid
120.5
3-oxochenodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
151.5
3-oxochenodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
60.4
3-oxocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
76.85
3-oxocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
101.7
3-oxodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
131
3-oxodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
84.3
3-oxolithocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
93.58
3-oxolithocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.17
chenodeoxycholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.45
chenodeoxycholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
8.7
chenodeoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
15.4
chenodeoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.12
cholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
0.23
cholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
5.22
choloyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
11.6
choloyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
9.32
deoxycholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
54.2
deoxycholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.72
glycocholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
1
glycocholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
10.18
lithocholoyl-CoA
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
33.3
lithocholoyl-CoA
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
13.17
NAD+
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
17.7
NAD+
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
18.5
NAD+
mutant E42A of isoform BaiA2, at pH 8.7 and 37°C
0.13
NADP+
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
0.3
NADP+
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
3.3
NADP+
mutantE42A of isoform BaiA2, at pH 8.7 and 37°C
0.73
taurocholic acid
wild type enzyme isoform BaiA2, at pH 8.7 and 37°C
1
taurocholic acid
wild type enzyme isoform BaiA1, at pH 8.7 and 37°C
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Mallonee, D.H.; Lijewski, M.A.; Hylemon, P.B.
Expression in Escherichia coli and characterization of a bile acid-inducible 3alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708
Curr. Microbiol.
30
259-263
1995
Eubacterium sp., Eubacterium sp. VPI 12708
brenda
Gopal-Srivastava, R.; Mallonee, D.H.; White, W.B.; Hylemon, P.B.
Multiple copies of a bile acid-inducible gene in Eubacterium sp. strain VPI 12708
J. Bacteriol.
172
4420-4426
1990
Eubacterium sp., Eubacterium sp. VPI 12708
brenda
Mallonee, D.H.; Hylemon, P.B.
Use of a short A/T-rich cassette for enhanced expression of cloned genes in Escherichia coli
Mol. Biotechnol.
11
27-35
1999
Eubacterium sp., Eubacterium sp. VPI 12708
brenda
Bhowmik, S.; Jones, D.H.; Chiu, H.P.; Park, I.H.; Chiu, H.J.; Axelrod, H.L.; Farr, C.L.; Tien, H.J.; Agarwalla, S.; Lesley, S.A.
Structural and functional characterization of BaiA, an enzyme involved in secondary bile acid synthesis in human gut microbe
Proteins
82
216-229
2014
[Clostridium] scindens (P19337), [Clostridium] scindens VPI 12708 (P19337)
brenda