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IUBMB Comments The enzyme is involved in the biosynthesis of several deoxysugars, including L -digitoxose, L - and D -olivose, L -oliose, D -mycarose and forosamine.
Synonyms
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DTDP-3,4-diketo-2,6-dideoxyglucose 3-ketoreductase
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dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose 3-oxidoreductase
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dTDP-4-keto-2,6-dideoxy-D-glucose 3-oxidoreductase
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NDP-hexose 3-ketoreductase
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dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+ = dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
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MetaCyc
dTDP-alpha-D-forosamine biosynthesis, dTDP-alpha-D-olivose, dTDP-alpha-D-oliose and dTDP-alpha-D-mycarose biosynthesis, dTDP-beta-L-digitoxose biosynthesis, dTDP-beta-L-olivose biosynthesis
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dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose:NADP+ 3-oxidoreductase
The enzyme is involved in the biosynthesis of several deoxysugars, including L-digitoxose, L- and D-olivose, L-oliose, D-mycarose and forosamine.
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dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
dTDP-3-dehydro-6-deoxy-alpha D-galactose + NADPH + H+
dTDP-fucose + NADP+
Substrates: -
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dTDP-3-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
dTDP-quinovose + NADP+
Substrates: -
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TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
additional information
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dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
Substrates: the enzyme is involved in the biosynthesis of dTDP-L-digitoxose
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dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
Substrates: dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose =(2R,6S)-6-hydroxy-2-methyldihydro-2H-pyran-3,4-dione
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TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
Substrates: -
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TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
Substrates: -
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additional information
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Substrates: to functioning on its natural substrate, KijD10 can also turn over sugars that contain hydroxyl groups at the C-2 and C-4 positions. It does not discriminate with respect to the orientation of the hydroxyl group at C-4
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additional information
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Substrates: to functioning on its natural substrate, KijD10 can also turn over sugars that contain hydroxyl groups at the C-2 and C-4 positions. It does not discriminate with respect to the orientation of the hydroxyl group at C-4
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dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
Substrates: the enzyme is involved in the biosynthesis of dTDP-L-digitoxose
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TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
Substrates: -
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NADH
NADPH is preferred over NADH
NADPH
preferred over NADH
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additional information
additional information
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additional information
additional information
kinetic data are determined for mutant enzymes and wild-type enzyme
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additional information
additional information
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kinetic data are determined for mutant enzymes and wild-type enzyme
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additional information
additional information
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additional information
additional information
kinetic data are determined for mutant enzymes and wild-type enzyme
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additional information
additional information
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kinetic data are determined for mutant enzymes and wild-type enzyme
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additional information
additional information
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additional information
additional information
kinetic data are determined for mutant enzymes and wild-type enzyme
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additional information
additional information
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kinetic data are determined for mutant enzymes and wild-type enzyme
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UniProt
brenda
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UniProt
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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physiological function
the enzyme is involved in the biosynthesis of dTDP-L-digitoxose
physiological function
enzyme is involved in insecticide spinosyn synthesis. It acts sequentially with 2,3-dehydratase SpnO to catalyze C-2 deoxygenation of TDP-4-dehydro-6-deoxy-D-glucose to form the SpnQ substrate, TDP-4-dehydro-2,6-dideoxy-D-glucose
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KIJDR_ACTKI
332
0
37033
Swiss-Prot
other Location (Reliability: 2 )
SPNN_SACSN
332
0
36133
Swiss-Prot
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OLEW_STRAT
328
0
35835
Swiss-Prot
other Location (Reliability: 1 )
GRA26_STRVN
342
0
36109
Swiss-Prot
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Q939Q8_STRVP
Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
332
0
34266
TrEMBL
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35834
x * 35834, calculated
37732
1 * 38000, SDS-PAGE, 1 * 37732, calculated, His-tagged recombinant protein
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monomer
1 * 38000, SDS-PAGE, 1 * 37732, calculated, His-tagged recombinant protein
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hanging drop method of vapor diffusion, binary and ternary complexes of KijD10 with NADP+ and dTDP-benzene, 2.0 A resolution or better
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K102A
mutant protein is completely inactive
K102E
mutant enzyme retains some activity, but its catalytic efficiency is reduced by more than 4 orders of magnitude from that for the wild-type enzyme
K102M
mutant protein is completely inactive
K102Q
mutant protein is completely inactive
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expression in Escherichia coli
expression in Streptomyces albus
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expression of the genes required for the biosynthesis of dTDP-L-olivose and dTDP-L-oleandrose in Streptomyces albus and incubation with the erythromycin aglycon erythronolide B gives rise to L-3-O-olivosyl- and L-3-O-oleandrosyl-erythronolide B
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Kubiak, R.L.; Holden, H.M.
Combined structural and functional investigation of a C-3''-ketoreductase involved in the biosynthesis of dTDP-L-digitoxose
Biochemistry
50
5905-5917
2011
Actinomadura kijaniata (B3TMR8), Actinomadura kijaniata
brenda
Aguirrezabalaga, I.; Olano, C.; Allende, N.; Rodriguez, L.; Brana, A.; Mendez, C.; Salas, J.
Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus
Antimicrob. Agents Chemother.
44
1266-1275
2000
Streptomyces antibioticus (Q9RR32)
brenda
Hong, L.; Zhao, Z.; Melancon III, C.; Zhang, H.; Liu, H.
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa
J. Am. Chem. Soc.
130
4954-4967
2008
Saccharopolyspora spinosa (Q9ALN5)
brenda
Wang, L.; White, R.; Vining, L.
Biosynthesis of the dideoxysugar component of jadomycin B: Genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone
Microbiology
148
1091-1103
2002
Streptomyces venezuelae (Q939Q8)
brenda
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