two firmly bound zinc atoms per subunit. All three ligands, C40, H62 and C158 to the catalytic zinc atom in the active-site, and all four ligands, C98, C101, C104 and Cll2 to the second zinc atom, are conserved
Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. The enzymes responsible for early steps in D-camphor degradation are 5-exo-hydroxycamphor dehydrogenase (camD gene), cytochrome P-450cam (camC), NADH-putidaredoxin reductase (camA), and putidaredoxin (camB)
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene camD encoding 5-exo-hydroxycamphor dehydrogenase is plasmid born, cotransferable by conjugation, mitomycin curable, and shows a higher segregation rate from cells that are multiplasmid rather than carrying a single plasmid
the genes of the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor form an operon, camDCAB, and are under negative control by the gene camR located immediately upstream from the camD gene
synthetic route for the synthesis of bifunctional camphor derivatives. The combination of the enzymatic P450cam system with 5-exo-hydroxycamphor dehydrogenase (FdeH) allows an efficient synthesis of 2,5-diketobornane, which can be used for further derivatisation
Koga, H.; Yamaguchi, E.; Matsunaga, K.; Aramaki, H.; Horiuchi, T.
Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida
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