Information on EC 1.1.1.156 - glycerol 2-dehydrogenase (NADP+)

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: involved in synthesis of glycerol during early spore germination
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

286041, 587013

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Schizosaccharomyces slooffiae

Substrates: -
Products: -

L-glyceraldehyde + NADPH

L-glycerol + NADP+

Substrates: very low activity
Products: -

L-glyceraldehyde + NADPH

L-glycerol + NADP+

Substrates: very low activity
Products: -

meso-erythritol + NADP+

? + NADPH

Substrates: 0.4% of the activity compared to glycerol
Products: -

meso-erythritol + NADP+

? + NADPH

Substrates: 35% of the activity compared to glycerol
Products: -

show all columns Go to Natural Substrate Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

glycerol + NADP+

dihydroxyacetone + NADPH + H+

8 entries

glycerol + NADP+

dihydroxyacetone + NADPH + H+

81028

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: low affinity for glycerol and high affinity for dihydroxyacetone promotes synthesis and accumulation of glycerol as the major osmotic regulator of the cell
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: involved in synthesis of glycerol during early spore germination
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

286041, 587013

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Substrates: -
Products: -

glycerol + NADP+

dihydroxyacetone + NADPH + H+

Schizosaccharomyces slooffiae

Substrates: -
Products: -

show all columns Go to Cofactor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NADP+

NADPH

NADP+

NADP+

can not be replaced by NAD+

286040, 286041, 587013

NADP+

can not be replaced by NAD+

NADP+

Schizosaccharomyces slooffiae

can not be replaced by NAD+

NADP+

can not be replaced by NAD+

NADP+

can not be replaced by NAD+

NADPH

NADPH

show all columns Go to Metals and Ions Search

dependent on

NADPH

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

NaCl

forward reaction highest activity at 4.5 M, reverse reaction at 3.5 M

show all columns Go to Inhibitor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-mercaptoethanol

80% inhibition at 1 mM

8-hydroxyquinoline

complete inhibition at 10 mM

dithiothreitol

25% inhibition at 10 mM

KCl

inhibition above 100 mM

L-cysteine

40% inhibition at 10 mM

Mg2+

40% inhibition at 5 mM

Mn2+

25% inhibition at 5 mM

p-chloromercuribenzoate

strong inhibition

show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0083 - 3.9

dihydroxyacetone

5 entries

51 - 2437

glycerol

4 entries

0.015 - 0.167

NADP+

4 entries

0.004 - 0.34

NADPH

4 entries

0.0083

dihydroxyacetone

at pH 6.5, in 50 mM potassium phosphate buffer, at 25°C

0.67

dihydroxyacetone

0.8

dihydroxyacetone

dihydroxyacetone

pH 7.0, 30°C

3.9

dihydroxyacetone

glycerol

350

glycerol

pH 9.5, 30°C

1400

glycerol

2437

glycerol

0.015

NADP+

0.1

NADP+

0.11

NADP+

pH 9.5, 30°C

0.167

NADP+

0.004

NADPH

0.01

NADPH

0.05

NADPH

pH 7.0, 30°C

0.34

NADPH

show all columns Go to Specific Activity Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.06

crude extract, at 25°C

0.54

0.67

0.73

13.71

after 228.5fold purification, at 25°C

15.7

31.1

show all columns Go to pH Optimum Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

dihydroxyacetone reduction

6 - 6.5

dihydroxyacetone reduction

7.4

assay reverse reaction

7.5

dihydroxyacetone reduction

7.8

assay at

glycerol oxidation

9.2

assay forward reaction

9.4

glycerol oxidation

9.5

glycerol oxidation

show all columns Go to Temperature Optimum Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Cucumis sativus

assay at

761196

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

brenda

brenda

brenda

Gluconobacter oxydans IFO 3255

brenda

brenda

brenda

low levels of activity

brenda

286040, 286041, 587013

brenda

Schizosaccharomyces slooffiae

low levels of activity

brenda

also known as hypocrea jecorina

SwissProt

brenda

show all columns Go to Source Tissue Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

6 entries

brenda

brenda

81028, 286042

brenda

brenda

286040, 587013

brenda

induction of enzyme synthesis by growth in glycerol- or dihydroxyacetone-containing media

brenda

brenda

Schizosaccharomyces slooffiae

brenda

81028, 286042

brenda

brenda

286040, 587013

brenda

induction of enzyme synthesis by growth in glycerol- or dihydroxyacetone-containing media

brenda

brenda

Schizosaccharomyces slooffiae

brenda

show all columns Go to Localization Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

cytoplasm

2 entries

cytoplasm

5737

Gluconobacter oxydans IFO 3255

brenda

cytoplasm

5737

brenda

top hide

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

show all columns Go to General Information Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

metabolism

under osmotic stress, G3pdh and Dhar play important roles in glycerol accumulation or degradation in Dunaliella for osmotic adjustment to respect to various salinities

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

P14065 pBLAST

GCY1_YEAST

Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

312

35079

Swiss-Prot

Show Sequence

Q0GYU4 pBLAST

GLD2_HYPJE

Hypocrea jecorina

318

35721

Swiss-Prot

Show Sequence

show all columns Go to Molecular Weight Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

25000

1 * 30000 + 1 * 25000, SDS-PAGE

30000

2 entries

34000 - 39000

gel filtration, SDS-PAGE

35660

39000

1 * 39000, SDS-PAGE

57000

gel filtration

30000

SDS-PAGE

30000

1 * 30000 + 1 * 25000, SDS-PAGE

show all columns Go to Subunit Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

dimer

1 * 30000 + 1 * 25000, SDS-PAGE

monomer

1 * 39000, SDS-PAGE

show all columns Go to Temperature Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

little loss of activity

complete inactivation

Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

DEAE-cellulose column chromatography, hydroxyapatite column chromatography, BD Sepharose column chromatography, and DEAE-Sephadex A-50 gel filtration

partial

to homogeneity, 1step chromatography

to homogeneity, chromatography techniques

Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Saccharomyces cerevisiae

Go to Expression Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

EXPRESSION

ORGANISM

UNIPROT

LITERATURE

activity increases at the beginning of salt stress, up to 1.9fold increase. Hypoosmotic stress also induces the activity

Cucumis sativus

761196

top print hide References Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

81028

Gimmler, H.; Lotter, G.

The intracellular distribution of enzymes of the glycerol cycle in the unicellular alga Dunaliella parva

Z. Naturforsch. C

1107-1114

1982

Dunaliella parva

brenda

Marshall, J.H.; Kong, Y.C.; Sloan, J.; May, J.W.

Purification and properties of glycerol:NADP+ 2-oxidoreductase from Schizisaccharomyces pombe

J. Gen. Microbiol.

135

697-701

1989

Schizosaccharomyces pombe

2621446

brenda

Vasiliadis, G.E.; Sloan, J.; Marshall, J.H.; May, J.W.

Glycerol and dihydroxyacetone metabolizing enzymes in fission yeasts of the genus Schizosaccharomyces

Arch. Microbiol.

147

263-267

1987

Schizosaccharomyces pombe, Schizosaccharomyces octosporus, Schizosaccharomyces slooffiae

brenda

Amotz-Ben, A.; Avron, M.

NADP specific dihydroxyacetone reductase from Dunaliella parva

FEBS Lett.

153-155

1973

Dunaliella parva

4146296

brenda

Van Laere, A.

Purification and properties of an NADPH-dependent dihydroxyacetone reductase from Phycomyces spores

FEMS Microbiol. Lett.

377-381

1985

Phycomyces blakesleeanus

brenda

Kong, Y.C.; May, J.W.; Marshall, J.H.

Glycerol oxidation and triose reduction by pyridine nucleotide-linked enzyme in the fission yeast Schizosaccharomyces pombe

J. Gen. Microbiol.

131

1571-1579

1985

Schizosaccharomyces pombe

brenda

Liepins, J.; Kuorelahti, S.; Penttilae, M.; Richard, P.

Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina

FEBS J.

273

4229-4235

2006

Trichoderma reesei (Q0GYU4), Trichoderma reesei

16930134

brenda

Adachi, O.; Ano, Y.; Shinagawa, E.; Matsushita, K.

Purification and properties of two different dihydroxyacetone reductases in Gluconobacter suboxydans grown on glycerol

Biosci. Biotechnol. Biochem.

2124-2132

2008

Gluconobacter oxydans, Gluconobacter oxydans IFO 3255

18685208

brenda