Information on EC 1.1.1.149 - 20alpha-hydroxysteroid dehydrogenase

additional information

?

-

3-acetylpyridine adenine dinucleotide phosphate

-

coenzyme analog

NAD(P)+

10 entries

NAD(P)H

10 entries

NAD+

-

NADH

NADP+

NADPH

nicotinamide 1,N6-ethenoadenine dinucleotide phosphate

-

coenzyme analog

nicotinamide hypoxanthine dinucleotide phosphate

-

coenzyme analog

thio-NADP+

-

coenzyme analog

additional information

-

nitroblue tetrazolium as hydrogen acceptor

-

K+

-

activates

Mg2+

Mn2+

-

required

285994

1,10-phenanthroline

-

50% inhibition at 0.065 mM

1,2-naphthoquinone

-

0.01 mM, 55% inhibition of enzyme in liver, 10% in kidney, 10% in lung cytosolic fraction, respectively

688031

1-phenylcyclopentanecarboxylic acid

-

16alpha-Bromoacetoxyprogesterone

-

285977

17alpha-hydroxyprogesterone

-

most inhibitory at pH 8.0, significant at pH 6.8

17beta-estradiol

-

20alpha-hydroxyprogesterone analog, competitive or noncompetitive depending on variable substrate

2',5'-adenosine bisphosphate

-

competitive inhibition versus NADP+

2',5'-ADP

-

coenzyme-competitive inhibitor

2'-adenylic acid

-

coenzyme-competitive inhibitor

2'-phosphoadenosine diphosphoribose

2,4-Pentanedione

-

pH profile for the inactivation, overview

2-(4-chlorobenzylidene)cyclopentanone

-

2-(4-chlorobenzylidene)cyclopentyl ethyl ether

-

2-acetylbenzofuran

-

50% inhibition at 0.033 mM

20beta-hydroxypregn-4-en-3-one

-

marginally inhibitory at pH 8.0

3',3'',5',5''-tetrabromophenolphthalein

3',3'',5',5''-tetrabromophenolphthalein diacetate

-

50% inhibition at 350 nM

3',3'',5',5''-tetraiodophenolphthalein

-

50% inhibition at 120 nM

3',5'-cyclic AMP

-

coenzyme-competitive inhibitor

3,5-dibromo-4-hydroxybenzoic acid methyl ester

-

50% inhibition at 0.099 mM

3,5-dibromosalicylic acid

-

3,5-dichlorosalicylic acid

3,5-diiodosalicylic acid

-

competitive

3-Aminopyridine 1,N6-ethenoadenine dinucleotide phosphate

-

NADP analog, competitive inhibitor

3-Aminopyridine adenine dinucleotide phosphate

3-bromo-5-phenylsalicylic acid

3-chloro-5-phenylsalicylic acid

3-phenyl-5-bromosalicylic acid

-

3-phenylcyclopentanecarboxylic acid

-

4'-butyl-5-chloro-4-hydroxybiphenyl-3-carboxylic acid

7fold selectivity relative to 3alpha-hydroxysteroid dehydrogenase AKR1C2

5'-adenosine diphosphate

-

5'-Adenosine triphosphate

-

5'-adenylic acid

-

coenzyme-competitive inhibitor

5'-ADP

-

coenzyme-competitive inhibitor

5'-ATP

-

coenzyme-competitive inhibitor

5-chloro-4-hydroxy-3'-methylbiphenyl-3-carboxylic acid

13fold selectivity relative to 3alpha-hydroxysteroid dehydrogenase AKR1C2

5-chloro-4-hydroxy-4'-methylbiphenyl-3-carboxylic acid

24fold selectivity relative to 3alpha-hydroxysteroid dehydrogenase AKR1C2

5-chloro-4-hydroxybiphenyl-3-carboxylic acid

24fold selectivity relative to 3alpha-hydroxysteroid dehydrogenase AKR1C2

5-fluoro-4-hydroxybiphenyl-3-carboxylic acid

1.2fold selectivity relative to 3alpha-hydroxysteroid dehydrogenase AKR1C2

5alpha-dihydrotestosterone

-

285981

5beta-dihydrotestosterone

-

285981

9,10-phenanthrenequinone

-

0.01 mM, 70% inhibition of enzyme in liver, 5% in kidney, 60% in lung cytosolic fraction, respectively

688031

Acetylsalicylate

-

adenosine 2'-monophospho- 5'-diphosphoribose

-

strong inhibition

adenosine diphosphoribose

-

coenzyme-competitive inhibitor

Ag+

-

very powerful

aminopyridine 1,N6-ethenoadenine dinucleotide phosphate

-

aminopyridine adenine dinucleotide phosphate

-

androstendione

-

most inhibitory at pH 8.0

apigenin

barbiturates

-

-

benzbromarone

benzbromarone acetate

-

50% inhibition at 430 nM

benzofuran

-

50% inhibition at 1.45 mM

benzofuran-2-carboxylic acid

-

50% inhibition at 0.16 mM

Bethamethasone

-

0.1 mM, 10% inhibition

Bile acids

Bromophenol blue

carboxylic acid

-

competitive for acetic and propionic acid, mixed-type from pentanoic to undecanoic acid with exception of heptanoic acid

cibacron

cloxazolam

-

0.01 mM, 38% inhibition

Cu2+

diazepam

-

50% inhibition at 0.0056 mM

Diazotized 3-aminopyridine 1,N6-ethenoadenine dinucleotide phosphate

-

NADP+ analog, competitive inhibitor

Diazotized 3-aminopyridine adenine dinucleotide phosphate

-

NADP analog, competitive inhibitor

dibenzofuran

-

50% inhibition at 0.895 mM

Dienstrol

Diphenic acid

-

less than 17% inhibition

ethyl acetate

fatty acids

fisetin

Flufenamic acid

Fluorescein mercuric acetate

-

galangin

-

glycerol

-

inhibition partially reversed by cysteine

285986

glycyrrhetinic acid

-

50% inhibition at 0.0022 mM

Hexestrol

indomethacin

kaempferol

linear monocarboxylic acids

-

slight inhibition, overview

-

linear N-alkylammonium chloride derivatives

-

slight inhibition, overview

-

lithocholic acid

-

50% inhibition at 0.022 mM

luteolin

Maleimide

-

pH profile for the inactivation, overview

Meclofenamic acid

-

20alpha-hydroxyprogesterone oxidation

medazepam

-

50% inhibition at 0.0047 mM

Medroxyprogesterone acetate

-

50% inhibition at 700 nM

Mefenamic acid

-

Metal ions

-

strong

-

myricetin

N-1-butylnicotinamide chloride

-

inhibition of the ovarian enzyme, competitive inhibition versus NADP+

N-1-decylnicotinamide chloride

-

inhibition of the ovarian enzyme, mixed type inhibition versus NADP+

N-1-dodecylnicotinamide chloride

-

inhibition of the ovarian enzyme, mixed type inhibition versus NADP+

N-1-ethylnicotinamide chloride

-

inhibition of the ovarian enzyme, competitive inhibition versus NADP+

N-1-heptylnicotinamide chloride

-

inhibition of the ovarian enzyme, competitive inhibition versus NADP+

N-1-hexylnicotinamide chloride

-

inhibition of the ovarian enzyme, competitive inhibition versus NADP+

N-1-methylnicotinamide chloride

-

inhibition of the ovarian enzyme, competitive inhibition versus NADP+

N-1-nonylnicotinamide chloride

-

inhibition of the ovarian enzyme, mixed type inhibition versus NADP+

N-1-octylnicotinamide chloride

-

inhibition of the ovarian enzyme, mixed type inhibition versus NADP+

N-1-pentylnicotinamide chloride

-

inhibition of the ovarian enzyme, competitive inhibition versus NADP+

N-1-propylnicotinamide chloride

-

inhibition of the ovarian enzyme, competitive inhibition versus NADP+

N-1-undecylnicotinamide chloride

-

inhibition of the ovarian enzyme, mixed type inhibition versus NADP+

N-alkylammonium chloride

-

competitive from pentyl to heptyl, mixed type from nonyl to dodecyl

N-Alkylmaleimide

-

N-ethylmaleimide and N-butylmaleimide to N-octylmaleimide at 25°C, pH 7.7

N-alkylmaleimides

-

coenzyme-competitive inhibition, varying in chainlength from N-ethyl- to N-octylmaleimide, effectively inactivated the enzyme through pseudo-first-order rate processes, overview

-

N-Butylmaleimide

-

inactivation via sulfhydryl groups of the enzyme in a nonpolar region

N-ethylmaleimide

-

inactivation via sulfhydryl groups of the enzyme in a nonpolar region

N-Heptylmaleimide

-

inactivation via sulfhydryl groups of the enzyme in a nonpolar region

N-Hexylmaleimide

-

inactivation via sulfhydryl groups of the enzyme in a nonpolar region

N-Octylmaleimide

-

inactivation via sulfhydryl groups of the enzyme in a nonpolar region

N-Pentylmaleimide

-

inactivation via sulfhydryl groups of the enzyme in a nonpolar region

N1-alkylnicotinamide chloride

-

competitive from methyl to heptyl, mixed-type from octyl to dodecyl with respect to NADP

NAD+

-

NADP analog, competitive inhibitor

NADH

-

concentrations above 0.1 mM

NADP+

NADPH

-

competitive and noncompetitive product inhibition with respect to NADP+ depending on substrate concentrations

naringenin

natural oestrogens

-

e.g. oestrone less than 15% inhibition

-

o-cresolphthalein

-

50% inhibition at 140 nM

p-chloromercuribenzoate

-

also other sufhydryl reagents like 5,5'-dithiobis-2-nitrobenzoic acid and iodoacetamide strong

phenazine methosulfate

-

0.01-0.3 mg/ml

Phenobarbital

Phenolphthalein

progesterone

prolactin

-

down-regulating of gene-expression

-

pyridoxal 5'-phosphate

-

strong, 90% inactivation by 10 mM

quercetin

quercitrin

salicylate

-

sorbinil

Sulfobromophthalein

synthetic non-steroidal oestrogens

-

hexoestrol most potent competitive inhibitor

-

synthetic oestrogens

-

e.g. hexestrol, silbestrol, dienstrol

-

Tolrestat

Ursodeoxycholic acid

-

50% inhibition at 0.34 mM

valporic acid

-

less than 17% inhibition

additional information

8 entries

-

cysteine

-

restoring, also other sulfhydryl compounds, NADH-linked to a greater extent than the NADPH-linked activity

Dimethyl formamide

-

5-10%

additional information

-

0.131

(S)-1-indanol

-

at pH 7.4 and 37°C

0.112

(S)-1-tetralol

-

at pH 7.4 and 37°C

1 - 1870

(S)-ethyl 3-hydroxybutyrate

4.8 - 5

(S)-methyl 3-hydroxybutyrate

0.007 - 0.47

(S)-tetralol

0.092 - 0.129

1,2-Cyclohexanedione

0.006

1-phenyl-1,2-propanedione

0.0033

11-deoxycorticosterone

-

pH 7.4, 37°C

668428

0.073

17-hydroxyprogesterone

-

0.013

17alpha,20alpha-dihydroxypregn-4-en-3-one

-

0.013 - 0.014

17alpha,20alpha-dihydroxyprogesterone

0.022

17alpha,21-dihydroxy-5beta-pregnan-3,20-dione

-

0.02

17alpha,21-dihydroxy-5beta-pregnane-3,11,20-trione

-

0.002 - 0.0034

17alpha-hydroxypregnenolone

0.0029 - 0.118

17alpha-hydroxyprogesterone

8 entries

0.0013

17beta-estradiol

-

at pH 7.4 and 37°C

34.8 - 66.3

2,3-Butanediol

0.015 - 0.018

2,3-Butanedione

0.005 - 0.008

2,3-heptanedione

0.01 - 0.016

2,3-Hexanedione

0.018 - 0.021

2,3-Pentanedione

0.131 - 0.243

2,4-Pentanedione

0.0026

2-amino-8-((2-fluoro-4-methoxybenzyl)thio)-1,9-dihydro-6H-purin-6-one

-

pH 7.0, 25°C

0.001

2-tert-butyl-1,4-benzoquinone

-

at pH 7.4 and 37°C

0.041

20alpha-dihydrocortisol

-

NAD+, pH 7.9

0.00102 - 0.00516

20alpha-hydroxy-4-pregnen-3-one

0.013

20alpha-hydroxy-5alpha-pregnan-3-one

-

+/-0.0035

0.073

20alpha-hydroxy-5beta-pregnan-3-one

-

0.00117 - 2.5

20alpha-hydroxyprogesterone

11 entries

0.23 - 0.57

3,4-hexanedione

0.00208

3-acetylpyridine adenine dinucleotide phosphate

-

NADP analog

1.4

3-deoxyglucosone

-

at pH 7.4 and 37°C

0.012

3alpha-hydroxy-5alpha-pregnan-20-one

-

+/-0.0035

0.001

4-androstene-3,17-dione

-

at pH 7.4 and 37°C

0.152

4-chromanol

-

at pH 7.4 and 37°C

0.0053

4-hydroxy-2-nonenal

-

at pH 7.4 and 37°C

0.0012

4-Nitroacetophenone

-

at pH 7.4 and 37°C

0.221 - 0.3811

4-nitrobenzaldehyde

0.0059

4-oxo-2-hexenal

-

at pH 7.4 and 37°C

0.0018

4-oxo-2-nonenal

-

at pH 7.4 and 37°C

0.016

4-pregnen-20alpha-ol-3-one

-

pH 7.0, 25°C

0.001

4-pregnen-3alpha-ol-20-one

-

at pH 7.4 and 37°C

0.0031

5-androstene-3beta,17beta-diol

-

at pH 7.4 and 37°C

0.0042

5alpha-androstan-3alpha,17beta-diol

-

at pH 7.4 and 37°C

0.0041

5alpha-Androstan-3alpha-ol-17-one

-

at pH 7.4 and 37°C

0.0022

5alpha-androstan-3beta,17beta-diol

-

at pH 7.4 and 37°C

0.006

5alpha-Androstan-3beta-ol-17-one

-

at pH 7.4 and 37°C

0.0034 - 0.018

5alpha-dihydrotestosterone

0.0007 - 0.011

5alpha-pregnan-20alpha-ol-3-one

0.0005

5alpha-pregnan-3,20-dione

-

at pH 7.4 and 37°C

0.0008

5alpha-pregnan-3alpha,20alpha-diol

-

at pH 7.4 and 37°C

0.0062

5alpha-Pregnan-3alpha,21-diol-20-one

-

at pH 7.4 and 37°C

0.0013 - 0.0033

5alpha-pregnan-3alpha-ol-20-one

0.0012

5alpha-pregnane-21-ol-3,20-dione

-

pH 7.0, 25°C

0.0011

5alpha-pregnane-3,20-dione

-

pH 7.0, 25°C

0.0004 - 0.0009

5alpha-pregnane-3alpha,20alpha-diol

0.0062

5beta-androstan-17beta-ol-3-one

-

at pH 7.4 and 37°C

0.0014

5beta-Androstan-3alpha,17beta-diol

-

at pH 7.4 and 37°C

0.0017

5beta-Androstan-3alpha-ol-17-one

-

at pH 7.4 and 37°C

0.0025

5beta-Androstan-3beta,17beta-diol

-

at pH 7.4 and 37°C

0.0028

5beta-androstan-3beta-ol-17-one

-

at pH 7.4 and 37°C

0.001 - 0.073

5beta-pregnan-20alpha-ol-3-one

0.001

5beta-pregnan-3,20-dione

-

at pH 7.4 and 37°C

0.0078

5beta-pregnan-3alpha,20alpha-diol

-

at pH 7.4 and 37°C

0.0026

5beta-Pregnan-3alpha,21-diol-20-one

-

at pH 7.4 and 37°C

0.001 - 0.0062

5beta-Pregnan-3alpha-ol-20-one

0.0012

5beta-pregnane-3,20-dione

-

pH 7.0, 25°C

0.0015

5beta-pregnane-3alpha,20alpha-diol

-

pH 7.0, 25°C

0.002

6-tert-butyl-2,3-epoxy-5-cyclohexen-1,4-dione

-

at pH 7.4 and 37°C

0.0029

7alpha-hydroxy-dehydroepiandrosterone

-

at pH 7.4 and 37°C

0.00195

9,10-phenanthrenequinone

-

+/-0.0004

0.02 - 0.022

acetoacetyl-CoA

0.162 - 0.272

acetoin

0.014

acrolein

-

at pH 7.4 and 37°C

0.0089

all-trans-retinal

-

at pH 7.4 and 37°C

0.053

alpha-tetralone

-

at pH 7.4 and 37°C

0.034

befunolol

-

at pH 7.4 and 37°C

2.1

benzene dihydrodiol

-

0.008

benzyl acetoacetate

0.018

beta-ionol

-

at pH 7.4 and 37°C

0.078

Camphorquinone

-

2.02 - 2.9

cis-benzene-1,2-dihydrodiol

0.02407 - 0.032

cortisol

0.022

cortisone

-

NADH, pH 6.3

120

D,L-glyceraldehyde

-

0.3 - 0.67

D-glyceraldehyde

0.534 - 0.587

D-Lactaldehyde

0.006

dehydroepiandrosterone

-

at pH 7.4 and 37°C

0.0042

dehydroepiandrosterone 3-sulfate

-

at pH 7.4 and 37°C

0.019

diacetyl

-

at pH 7.4 and 37°C

0.069 - 0.151

dihydrotestosterone

0.852 - 1.3

DL-glyceraldehyde 3-phosphate

0.0011

estrone

-

at pH 7.4 and 37°C

0.0067

Estrone 3-sulfate

-

at pH 7.4 and 37°C

0.012 - 0.015

ethyl 2-methylacetoacetate

0.222 - 0.447

ethyl 3-oxovalerate

0.027

ethyl 4-chloroacetoacetate

0.02 - 20

ethyl acetoacetate

0.303 - 0.654

ethyl benzoylacetate

0.025

ethyl pyruvate

0.18

geraniol

-

at pH 7.4 and 37°C

0.0076

hexanal

-

at pH 7.4 and 37°C

0.0006 - 0.064

isatin

0.068

isocaproaldehyde

-

at pH 7.4 and 37°C

0.111

ketotifen

-

at pH 7.4 and 37°C

0.018

menadione

-

at pH 7.4 and 37°C

0.117 - 0.123

methyl acetoacetate

0.088 - 0.548

methylglyoxal

0.004

n-butyl acetoacetate

0.0015 - 0.0024

n-hexyl acetoacetate

0.0013 - 0.0016

n-octyl acetoacetate

0.526

NAD+

-

20alpha-dihydrocortisol

0.0081 - 1.1

NADH

0.0007 - 0.025

NADP+

9 entries

0.0002 - 0.052

NADPH

9 entries

0.1429

nicotinamide 1,N6-ethenoadenine dinucleotide phosphate

-

NADP analog

0.0588

nicotinamide hypoxanthine dinucleotide phosphate

-

NADP analog

0.0009

Nonanal

-

at pH 7.4 and 37°C

0.43 - 0.591

Phenylglyoxal

0.0006 - 0.2833

progesterone

15 entries

0.061

pyridine 3-aldehyde

-

at pH 7.4 and 37°C

0.049 - 0.081

Pyridine-3-aldehyde

0.945 - 1.06

Pyridine-4-aldehyde

0.013

testosterone

-

at pH 7.4 and 37°C

0.00186

thionicotinamide adenine dinucleotide phosphate

-

NADP analog

50.9 - 79.7

trans-1S,2S-cyclohexanediol

0.0074

trans-2-hexenal

-

at pH 7.4 and 37°C

0.0055

trans-2-nonenal

-

at pH 7.4 and 37°C

0.26

trans-benzene dihydrodiol

-

at pH 7.4 and 37°C

1.6

trans-benzene-1S,2S-dihydrodiol

additional information

-

0.2

(S)-1-indanol

-

at pH 7.4 and 37°C

0.217

(S)-1-tetralol

-

at pH 7.4 and 37°C

0.178

17alpha-hydroxyprogesterone

-

+/-0.8

0.011

17beta-estradiol

-

at pH 7.4 and 37°C

0.183

2-tert-butyl-1,4-benzoquinone

-

at pH 7.4 and 37°C

0.785

20alpha-hydroxy-5alpha-pregnan-3-one

-

+/-4.3

0.04 - 1.17

20alpha-hydroxyprogesterone

0.183

3-deoxyglucosone

-

at pH 7.4 and 37°C

0.208

3alpha-hydroxy-5-alpha-pregnan-20-one

-

+/-1.6

0.043

4-androstene-3,17-dione

-

at pH 7.4 and 37°C

0.068

4-chromanol

-

at pH 7.4 and 37°C

0.009

4-hydroxy-2-nonenal

-

at pH 7.4 and 37°C

0.112

4-Nitroacetophenone

-

at pH 7.4 and 37°C

5.91

4-nitrobenzaldehyde

-

+/-33.4

0.0115

4-oxo-2-hexenal

-

at pH 7.4 and 37°C

0.112

4-oxo-2-nonenal

-

at pH 7.4 and 37°C

0.032

4-pregnen-3alpha-ol-20-one

-

at pH 7.4 and 37°C

0.1

5-androstene-3beta,17beta-diol

-

at pH 7.4 and 37°C

0.27

5alpha-androstan-3alpha,17beta-diol

-

at pH 7.4 and 37°C

0.085

5alpha-Androstan-3alpha-ol-17-one

-

at pH 7.4 and 37°C

0.032

5alpha-androstan-3beta,17beta-diol

-

at pH 7.4 and 37°C

0.083

5alpha-Androstan-3beta-ol-17-one

-

at pH 7.4 and 37°C

0.025 - 0.047

5alpha-dihydrotestosterone

0.077 - 0.158

5alpha-pregnan-20alpha-ol-3-one

0.018

5alpha-pregnan-3,20-dione

-

at pH 7.4 and 37°C

0.025

5alpha-pregnan-3alpha,20alpha-diol

-

at pH 7.4 and 37°C

0.018

5alpha-Pregnan-3alpha,21-diol-20-one

-

at pH 7.4 and 37°C

0.032

5alpha-pregnan-3alpha-ol-20-one

-

at pH 7.4 and 37°C

0.153

5beta-androstan-17beta-ol-3-one

-

at pH 7.4 and 37°C

0.25

5beta-Androstan-3alpha,17beta-diol

-

at pH 7.4 and 37°C

0.067

5beta-Androstan-3alpha-ol-17-one

-

at pH 7.4 and 37°C

0.033

5beta-Androstan-3beta,17beta-diol

-

at pH 7.4 and 37°C

0.045

5beta-androstan-3beta-ol-17-one

-

at pH 7.4 and 37°C

0.153

5beta-pregnan-20alpha-ol-3-one

-

at pH 7.4 and 37°C

0.028

5beta-pregnan-3,20-dione

-

at pH 7.4 and 37°C

0.283

5beta-pregnan-3alpha,20alpha-diol

-

at pH 7.4 and 37°C

0.017

5beta-Pregnan-3alpha,21-diol-20-one

-

at pH 7.4 and 37°C

0.03

5beta-Pregnan-3alpha-ol-20-one

-

at pH 7.4 and 37°C

0.167

6-tert-butyl-2,3-epoxy-5-cyclohexen-1,4-dione

-

at pH 7.4 and 37°C

0.043

7alpha-hydroxy-dehydroepiandrosterone

-

at pH 7.4 and 37°C

7.7

9,10-phenanthrenequinone

-

+/-26.9

0.097

acetoin

-

at pH 7.4 and 37°C

0.035

acrolein

-

at pH 7.4 and 37°C

0.0085

all-trans-retinal

-

at pH 7.4 and 37°C

0.053

alpha-tetralone

-

at pH 7.4 and 37°C

0.117

befunolol

-

at pH 7.4 and 37°C

0.233

beta-ionol

-

at pH 7.4 and 37°C

11.1

cortisol

pH 5.0, temperature not specified in the publication

0.215

D,L-glyceraldehyde

-

+/-0.3

0.155

dehydroepiandrosterone

-

at pH 7.4 and 37°C

0.08

dehydroepiandrosterone 3-sulfate

-

at pH 7.4 and 37°C

0.158

diacetyl

-

at pH 7.4 and 37°C

0.017

estrone

-

at pH 7.4 and 37°C

0.098

Estrone 3-sulfate

-

at pH 7.4 and 37°C

0.033

geraniol

-

at pH 7.4 and 37°C

0.043

hexanal

-

at pH 7.4 and 37°C

0.137

isatin

-

at pH 7.4 and 37°C

0.023

isocaproaldehyde

-

at pH 7.4 and 37°C

0.02

ketotifen

-

at pH 7.4 and 37°C

0.125

menadione

-

at pH 7.4 and 37°C

0.12

methylglyoxal

-

at pH 7.4 and 37°C

1.52

NADP+

-

+/-6.1

1.43

NADPH

-

+/-1.3

0.032

Nonanal

-

at pH 7.4 and 37°C

0.027 - 1.65

progesterone

0.132

pyridine 3-aldehyde

-

at pH 7.4 and 37°C

0.057

testosterone

-

at pH 7.4 and 37°C

0.025

trans-2-hexenal

-

at pH 7.4 and 37°C

0.02

trans-2-nonenal

-

at pH 7.4 and 37°C

0.108

trans-benzene dihydrodiol

-

at pH 7.4 and 37°C

1.5

(S)-1-indanol

-

at pH 7.4 and 37°C

2

(S)-1-tetralol

-

at pH 7.4 and 37°C

0.0000008 - 0.000062

(S)-tetralol

8.7

17beta-estradiol

-

at pH 7.4 and 37°C

183.3

2-tert-butyl-1,4-benzoquinone

-

at pH 7.4 and 37°C

21.7

20alpha-hydroxyprogesterone

-

at pH 7.4 and 37°C

0.13

3-deoxyglucosone

-

at pH 7.4 and 37°C

43.3

4-androstene-3,17-dione

-

at pH 7.4 and 37°C

0.45

4-chromanol

-

at pH 7.4 and 37°C

1.6

4-hydroxy-2-nonenal

-

at pH 7.4 and 37°C

93.3

4-Nitroacetophenone

-

at pH 7.4 and 37°C

1.8

4-oxo-2-hexenal

-

at pH 7.4 and 37°C

0.062

4-oxo-2-nonenal

-

at pH 7.4 and 37°C

31.7

4-pregnen-3alpha-ol-20-one

-

at pH 7.4 and 37°C

31.7

5-androstene-3beta,17beta-diol

-

at pH 7.4 and 37°C

63.3

5alpha-androstan-3alpha,17beta-diol

-

at pH 7.4 and 37°C

20

5alpha-Androstan-3alpha-ol-17-one

-

at pH 7.4 and 37°C

14.3

5alpha-androstan-3beta,17beta-diol

-

at pH 7.4 and 37°C

13.8

5alpha-Androstan-3beta-ol-17-one

-

at pH 7.4 and 37°C

2.7 - 7.3

5alpha-dihydrotestosterone

1.3 - 14.3

5alpha-pregnan-20alpha-ol-3-one

36.7

5alpha-pregnan-3,20-dione

-

at pH 7.4 and 37°C

30

5alpha-pregnan-3alpha,20alpha-diol

-

at pH 7.4 and 37°C

3

5alpha-Pregnan-3alpha,21-diol-20-one

-

at pH 7.4 and 37°C

25

5alpha-pregnan-3alpha-ol-20-one

-

at pH 7.4 and 37°C

0.00004 - 0.00027

5alpha-pregnane-3alpha,20alpha-diol

25

5beta-androstan-17beta-ol-3-one

-

at pH 7.4 and 37°C

183.3

5beta-Androstan-3alpha,17beta-diol

-

at pH 7.4 and 37°C

40

5beta-Androstan-3alpha-ol-17-one

-

at pH 7.4 and 37°C

13.3

5beta-Androstan-3beta,17beta-diol

-

at pH 7.4 and 37°C

16

5beta-androstan-3beta-ol-17-one

-

at pH 7.4 and 37°C

26.7

5beta-pregnan-20alpha-ol-3-one

-

at pH 7.4 and 37°C

28.3

5beta-pregnan-3,20-dione

-

at pH 7.4 and 37°C

36.7

5beta-pregnan-3alpha,20alpha-diol

-

at pH 7.4 and 37°C

6.3

5beta-Pregnan-3alpha,21-diol-20-one

-

at pH 7.4 and 37°C

0.0000007 - 30

5beta-Pregnan-3alpha-ol-20-one

83.3

6-tert-butyl-2,3-epoxy-5-cyclohexen-1,4-dione

-

at pH 7.4 and 37°C

15

7alpha-hydroxy-dehydroepiandrosterone

-

at pH 7.4 and 37°C

1.3

acetoin

-

at pH 7.4 and 37°C

2.5

acrolein

-

at pH 7.4 and 37°C

0.95

all-trans-retinal

-

at pH 7.4 and 37°C

1

alpha-tetralone

-

at pH 7.4 and 37°C

13.2

befunolol

-

at pH 7.4 and 37°C

10.1

beta-ionol

-

at pH 7.4 and 37°C

461.1

cortisol

pH 5.0, temperature not specified in the publication

26.7

dehydroepiandrosterone

-

at pH 7.4 and 37°C

18.3

dehydroepiandrosterone 3-sulfate

-

at pH 7.4 and 37°C

8.3

diacetyl

-

at pH 7.4 and 37°C

15.1

estrone

-

at pH 7.4 and 37°C

14.7

Estrone 3-sulfate

-

at pH 7.4 and 37°C

0.018

geraniol

-

at pH 7.4 and 37°C

5.7

hexanal

-

at pH 7.4 and 37°C

233.3

isatin

-

at pH 7.4 and 37°C

0.35

isocaproaldehyde

-

at pH 7.4 and 37°C

0.18

ketotifen

-

at pH 7.4 and 37°C

2

menadione

-

at pH 7.4 and 37°C

1.4

methylglyoxal

-

at pH 7.4 and 37°C

35

Nonanal

-

at pH 7.4 and 37°C

45

progesterone

-

at pH 7.4 and 37°C

2.2

pyridine 3-aldehyde

-

at pH 7.4 and 37°C

4.3

testosterone

-

at pH 7.4 and 37°C

3.3

trans-2-hexenal

-

at pH 7.4 and 37°C

3.7

trans-2-nonenal

-

at pH 7.4 and 37°C

0.42

trans-benzene dihydrodiol

-

at pH 7.4 and 37°C

0.0358

1-phenylcyclopentanecarboxylic acid

pH 6.5, temperature not specified in the publication

0.00134

2',5'-adenosine bisphosphate

-

pH 7.8, 23°C

0.148

2'-adenylic acid

-

pH 7.8, 23°C

0.0172

2-(4-chlorobenzylidene)cyclopentanone

pH 6.5, temperature not specified in the publication

0.0305

2-(4-chlorobenzylidene)cyclopentyl ethyl ether

pH 6.5, temperature not specified in the publication

0.0000007

3',3'',5',5''-tetrabromophenolphthalein

-

binding to NADPH-enzyme complex via ordered bi-bi-mechanism

0.000009

3,5-dibromosalicylic acid

-

0.0000059 - 0.0028

3,5-dichlorosalicylic acid

15 entries

0.000009

3,5-diiodosalicylic acid

-

0.000004 - 0.000113

3-bromo-5-phenylsalicylic acid

0.00000085 - 0.00007

3-chloro-5-phenylsalicylic acid

7 entries

0.00014

3-phenyl-5-bromosalicylic acid

-

0.0485

3-phenylcyclopentanecarboxylic acid

pH 6.5, temperature not specified in the publication

0.0000021

4'-butyl-5-chloro-4-hydroxybiphenyl-3-carboxylic acid

wild-type, 25°C, pH not specified in the publication

2.84

5'-adenylic acid

-

pH 7.8, 23°C

0.0000013

5-chloro-4-hydroxy-3'-methylbiphenyl-3-carboxylic acid

wild-type, 25°C, pH not specified in the publication

0.0000026

5-chloro-4-hydroxy-4'-methylbiphenyl-3-carboxylic acid

wild-type, 25°C, pH not specified in the publication

0.00000086

5-chloro-4-hydroxybiphenyl-3-carboxylic acid

wild-type, 25°C, pH not specified in the publication

0.0000013

5-fluoro-4-hydroxybiphenyl-3-carboxylic acid

wild-type, 25°C, pH not specified in the publication

0.021

Acetylsalicylate

-

0.00087

adenosine 2'-monophospho- 5'-diphosphoribose

-

pH 7.8, 23°C

0.95

adenosine diphosphoribose

-

pH 7.8, 23°C

0.34

ADP

-

pH 7.8, 23°C

0.01

aminopyridine 1,N6-ethenoadenine dinucleotide phosphate

-

pH 7.8, 23°C

0.0029

aminopyridine adenine dinucleotide phosphate

-

pH 7.8, 23°C

0.022

Diazotized 3-aminopyridine 1,N6-ethenoadenine dinucleotide phosphate

-

pH 7.8, 23°C

0.0005

Diazotized 3-aminopyridine adenine dinucleotide phosphate

-

pH 7.8, 23°C

9.29

N-1-butylnicotinamide chloride

-

pH 7.8, 23°C

1.24

N-1-decylnicotinamide chloride

-

pH 7.8, 23°C

8.95

N-1-ethylnicotinamide chloride

-

pH 7.8, 23°C

3.39

N-1-heptylnicotinamide chloride

-

pH 7.8, 23°C

6.59

N-1-hexylnicotinamide chloride

-

pH 7.8, 23°C

0.205 - 14.8

N-1-methylnicotinamide chloride

3.29

N-1-nonylnicotinamide chloride

-

pH 7.8, 23°C

4.01

N-1-octylnicotinamide chloride

-

pH 7.8, 23°C

9.35

N-1-pentylnicotinamide chloride

-

pH 7.8, 23°C

8.24

N-1-propylnicotinamide chloride

-

pH 7.8, 23°C

0.487

N-1-undecylnicotinamide chloride

-

pH 7.8, 23°C

0.00074

NADP+

-

pH 7.8, 23°C

0.0078

salicylate

-

additional information

-

0.0729

1-phenylcyclopentanecarboxylic acid

Homo sapiens

pH 6.5, temperature not specified in the publication

0.035

2-(4-chlorobenzylidene)cyclopentanone

Homo sapiens

pH 6.5, temperature not specified in the publication

0.0621

2-(4-chlorobenzylidene)cyclopentyl ethyl ether

Homo sapiens

pH 6.5, temperature not specified in the publication

0.003

3',3'',5',5''-tetrabromophenolphthalein

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.0986

3-phenylcyclopentanecarboxylic acid

Homo sapiens

pH 6.5, temperature not specified in the publication

0.00012

benzbromarone

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.026

Flufenamic acid

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.00041

galangin

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.0064

Hexestrol

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.00011

kaempferol

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.01

Mefenamic acid

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.00064

myricetin

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.00037

naringenin

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.000022

Phenolphthalein

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.000059

quercetin

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.011

Sulfobromophthalein

Oryctolagus cuniculus

-

at pH 7.4 and 37°C

0.002

-

reduction of 0.06 mM prostaglandin E2

0.007

-

reduction of 0.03 mM 17alpha-hydroxy-pregnenolone

0.012

-

+/-0.005

0.013

-

reduction of 100 mM D-glucose

0.016

-

reduction of 0.03 mM prednisolone

0.017

-

reduction of 100 mM D-galactose

0.024

-

+/-0.006

0.025

-

reduction of 0.03 mM prednisone

0.038 - 0.046

-

calculated from 17beta-estadiol dehydrogenase activity

285977

0.067

-

reduction of 0.05 mM pregnenolone

0.081

-

0.115

-

0.2596

-

insect cells

0.2769

-

HSD2, reduction of 17alpha-hydroxyprogesterone

0.282

-

measured with cortisone

0.332

-

+/-0.047, Escherichia coli

0.9

-

reduction of 0.06 mM 5alpha-pregnane-3,20-dione

1.3

pH 6.5, temperature not specified in the publication

1.462

-

HSD1, reduction of 17alpha-hydroxyprogesterone

2.09

-

20alpha-hydroxyprogesterone

2.5

-

oxidation of 0.06 mM 20alpha-hydroxy-5beta-pregnan-3-one

3.48

-

3.815

-

HSD2, reduction of progesterone

4.618

-

HSD1

5

-

6.2 - 6.9

-

6.515

-

HSD2

8.154

-

HSD1, reduction of progesterone

8.318

-

285985

additional information

10.5

-

steroid oxidation

5.5 - 7.5

-

NADPH

5.6

-

steroid reduction

6

-

renal NADH-dependent 20alpha-hydroxysteroid dehydrogenase

688681

6 - 6.5

6.5

-

hepatic NADPH-dependent 20alpha-hydroxysteroid dehydrogenase

688681

6.8 - 7.2

-

NADH

7.4

7.5

7.7

-

assay at

7.8

7.8 - 8.2

-

8

4 - 10

-

5.4 - 7.6

-

steroid reduction e.g. 20alpha-dihydrocortisol with MES, MOPS or sodium phosphate, NADH

5.8 - 10.5

-

25°C, below and above range inactivation

6.3 - 9

-

steroid oxidation e.g. cortisol with Tris hydrochloride, NAD+

6.5 - 9

-

pH 6.8 favouring progesterone to 20alpha-hydroxyprogesterone reaction and pH 8.0 vice versa

23

-

assay at

25

37

5

recombinant enzyme, reductive direction

5.5

recombinant enzyme, oxidative direction

50

-

reduction of 17alpha-hydroxyprogesterone

0 - 55

-

10% of maximal reaction rate at 0°C

-

Bifidobacterium adolescentis L2-32

UniProt

-

UniProt

female Shiba goats, 2-4 years old

-

-

SwissProt

-

-

-

-

Balb/c mice

-

-

654055, 740126

-

Rattus norvegicus Wistar-Imamichi

9 entries

female, Wistar-Imamichi strain

-

-

285986, 285987, 285988, 285990, 739860, 740522

-

-

285998, 285999

-

-

-

adipose tissue

endometrial cancer cell line

-

abdominal subcutaneous and omental adipose tissue, the enzyme activity is highest in women with visceral obesity, expression analysis

-

-

high expression level

-

breast cancer cells: tumorgenic strains MCF-7, MDA-MB-231, T-47D, nontumorgenic strain MCF-10A

-

placental cytotrophoblast villus

737453

-

-

-

fetal

-

-

20alpha-HSD mRNA highly expressed

-

-

moderate expression rate of AKR1C23

-

luminal epithelial cell

-

-

preovulatory, very high expression rate, expression analysis of AKR1C23, activity during follicular luteinization, overview

-

placental cytotrophoblast villus

-

-

20alpha-HSD mRNA lower expressed

the enzyme is specifically localized in the seminiferous tubule at 8 week

moderate expression rate of AKR1C23

very low expression rate of AKR1C23

low expression rate of AKR1C23

-

-

the enzyme is mainly localized in the trophoblast villus in the placenta on day 30 of pregnancy

-

uterine cervix carcinoma cell

-

placental cytotrophoblast villus

737453

additional information

-

amniotic fluid

-

670023

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

evolution

malfunction

metabolism

physiological function

7 entries

130500

gel filtration

160000

-

+/-2000 SDS-PAGE, PAGE, gel filtration-HPLC

30000

33000

34000

-

gel filtration

35000

37000

38000

-

1 * 38000, SDS-PAGE

39000

40000

41000

-

gel filtration, non-reducing conditions

48150

-

estimated by 1.3 kb cDNA

285997

68000

75100

-

thin layer gel filtration

?

dimer

homotetramer

monomer

tetramer

-

4 * 40000, identical, SDS-PAGE, N-terminal amino acid sequence

glycoprotein

phosphoprotein

sitting drop method, crystallization of the ternary complex of NADH and cortisol bound to the inactive S181A mutant, the structure is determined at 2.2 A for the apoform and 2.0 A for the binary complex with NADH

-

-

crystal structure of AKR1C1 complexed with the first structure-based designed inhibitor 3-chloro-5-phenylsalicylic acid bound in the active site is reported. The binding of 3-chloro-5-phenylsalicylic acid to AKR1C1 results in a conformational change in the side chain of Phe311 to accommodate the bulky phenyl ring substituent at the 5-position of the inhibitor

-

724521

docking model with substrates 5alpha-pregnane-3alpha,20alpha-diol and 5beta-pregnan-3alpha-ol-20-one. In the docked model of 5alpha-pregnane-3alpha,20alpha-diol the conformation of the steroid molecule is similar to that of 20alpha-hydroxyprogesterone in the crystal structure of the AKR1C1 complex where the steroid does not interact with the catalytic residues Tyr55 and His117. In the case of 5beta-pregnan-3alpha-ol-20-one the steroid interacts with the catalytic residue His117 and forms close contacts with Leu308

711423

in complex with NADP+ and progesterone

-

654055

in ternary complex with NADP+ and 3,5-dichlorosalicylic acid, hanging drop vapour diffusion method, using 25% (v/v) polyethylene glycol monomethyl ether 550, 0.02 M zinc sulfate in 0.1 M MES buffer (pH 6.5)

mutant L308V in complex with the inhibitor 3,5-dichlorosalicylic acid, to 1.9 A resolution. The inhibitor molecule is anchored from its carboxylate group that forms hydrogen bonds with the catalytic residues His117 and Tyr55, while the hydroxyl group is hydrogen bonded to His222. Van der Waals contacts are present between the inhibitor and residues Leu54, Leu306, and Phe311. Unlike the WT enzyme, the side-chain of the mutated Val308 makes long contacts with the inhibitor, the closest point of contact being 3.9 A

purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution

-

654055

ternary complex with NADP+ and 20alpha-hydroxyprogesterone

-

in complex with NADP+ and progesterone, with NADP+ and dihydrotestosterone, with NADP+ and 4-androstenedione

-

654055

purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 1.7-1.9 A resolution

-

-

-

isozyme HSD1, HSD2

-

-

S181A

inactive mutant enzyme

S183A

the kcat/Km-value for cortisol is 56% compared to wild-type enzyme

Bifidobacterium adolescentis L2-32

S181A

-

inactive mutant enzyme

-

S183A

Bifidobacterium adolescentis L2-32

-

the kcat/Km-value for cortisol is 56% compared to wild-type enzyme

-

D309L

-

similar Km-value as wild-type

E127D

-

increase in Km-value

F311A

-

mutation results in increased Ki values for 3-chloro-5-phenylsalicylic acid and 3,5-dichlorosalicylic acid compared to the wild-type enzyme

724521

F311L

-

mutation results in equal Ki value for 3-chloro-5-phenylsalicylic acid and increased Ki value for 3,5-dichlorosalicylic acid compared to the wild-type enzyme

724521

H222I

-

dramatic increase in Km-value

H222S

L306

the mutation in AKR1C1 results in a 46fold reduction in the potency of the inhibitor 3,5-dicholorosalicylic acid

L306A

-

mutation results in 81fold increase Ki values for 3-chloro-5-phenylsalicylic acid compared to the wild-type enzyme

L308A

L308V

L54V

R304L

-

dramatic increase in Km-value

T307V

-

similar Km-value as wild-type

Y305F

-

similar Km-value as wild-type

Y55F

-

no enzymic activity

additional information

8 entries

4.2

-

activity destroyed below

4.5 - 11.5

-

25°C, below and above range total loss of activity

5.5

-

at pH 5.5 for 30 min, after first purification step

20

-

30 min

25

37

-

6 min

38

-

half-life 7.6 min

4

40

-

up to, for 5 min 95% initial activity, rapidly inactivated above 45°C

45

-

rapidly inactivated above

55

-

rapidly inactivated

56

transition temperature. Important role of the 38-residue extended N-terminus in protein stability as deletion of the first 21 residues effectively abolishes enzyme activity with a 1.45 °C shift in Tm

60

-

10 min, pH 5.5, complete inactivation of activity of purification step 1 and 2

95

-

15 min, almost total loss of activity

additional information

2-mercaptoethanol for stabilization during purification

-

20%, v/v, glycerol for stabilization during purification

-

glycerol for prevention of large activity loss

-

NADP+ protects against thermal denaturation, steroid substrate does not

-

NADP+ protects against thermal inactivation, 20alpha-hydroxyprogesterone does not

-

significant activity loss by repeated thawing and freezing

-

-15°C, 0.005 mg/ml, 0.01 M potassium phosphate buffer, pH 8.0, 50% propylene glycol, 0.2 M KCl, 2 months

-

-20°C, porcine testes, 0.1 M phosphate buffer, pH 7.00.1 M phosphate buffer, pH 7.0, several months

-

-20°C, purified enzyme, 10 mM Tris/HCl, pH 7.5, 0.5 mM EDTA, 1 mM 2-mercaptoethanol, 20%, v/v, glycerol, 2 weeks, little activity loss

-

-70°C, 2.8 mg/ml protein concentration, 20 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 1 mM beta-mercaptoethanol, 30%, v/v, glycerol

-

-80°C, particulate and cytosolic fraction after homogenization

-

4 or -20°C, 2 mg/ml purified protein, 0.1 M phosphate buffer, pH 6-9, several days or for months

-

4°C or -20°C, above 0.5 mg of purified protein per ml, 50 mM phosphate buffer, pH 6.8, 10 mM 2-mercaptoethanol, 50% glycerol, v/v, several days

-

4°C or -20°C, ammonium sulfate fraction of purification step 2, over night or several months

-

4°C pH 6-7

-

4°C, cytosol preparation prior to purification, several months

-

285985

4°C, in presence of glycerol and DTT

-

4°C, Sephadex G-100 fraction, rapid decrease, 2 days, 50% activity loss, 80% activity remaining by addition of 20%, v/v, glycerol

-

glycerol

-

285986

no activity after single freeze-thaw of enzyme at any stage

-

-

affinity binding to glutathione-Sepharose and thrombin digestion of bacterial lysate, ion exchange and affinity chromatography of enzyme produced by insect cells

-

ammonium sulfate precipitation, affinity chromatography

-

285990

ammonium sulfate precipitation, affinity chromatography, gel filtration

-

ammonium sulfate precipitation, chromatography

-

285992

ammonium sulfate precipitation, ultrafiltration, chromatography

-

anion exchange and affinity chromatography

-

286001, 286003

anion exchange and affinity chromatography, gel filtration, HPLC

-

co-purification with 17beta-estradiol dehydrogenase, EC 1.1.1.62, affinity chromatography

-

285977

co-purification with aldehyde reductase and dihydrodiol dehydrogenase

-

column chromatography, gel filtration

five steps co-purification with aldehyde reductase and dihydrodiol dehydrogenase, ammonium sulfate precipitation, HPLC

-

gel filtration, affinity chromatography

-

285980, 285983

ion exchange chromatography

-

285985

isozyme HSD1 and HSD2, anion-exchange and affinity chromatography, capillary electrophoresis

-

partially by ammonium sulfate fractionation and chromatography

-

285996

recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity

Sepharose 6FF resin column chromatography

-

six step procedure, ammonium sulfate fractionation, chromatography, gel filtration

-

285986, 285987, 285988

solubilized, separated by SDS-PAGE, protein extraction from gel, renatured

-

cDNA of skin fibroblast cDNA library inserted into pCMV expression vector, transiently transfected into and expressed in HEK-293 cells

-

285997

DNA and amino acid sequence determination and analysis, expression analysis of AKR1C23 in preovulatory follicles, overexpression in HEK-293 cells

Equus caballus

Q1XAA8

669908

expressed in Escherichia coli as a His-tagged fusion protein

Macaca fascicularis

-

725822

expressed in Escherichia coli BL21(DE3) cells

-

expressed in Escherichia coli C41 (DE3)

-

expressed in Escherichia coli cells

expressed in Escherichia coli DH5alpha and baculovirus-insect cell systems Sf9

-

expressed in Escherichia coli JM109 cells

-

expressed in fission yeast Schizosaccharomyces pombe

-

725580

expression in Escherichia coli

711423

expression of soluble, active enzyme as GST-fusion enzyme in Escherichia coli

gene AKR1C1, real-time PCR expression anaysis

739864

overexpression in Escherichia coli BL21(DE3)RIPL

recombinantly expressed in Escherichia coli cells and CHO cells

-

726471

recombinantly expressed in fission yeast cells

-

723969

subcloned, overexpressed in Escherichia coli DH5alpha or C41 (DE3)

-

286003

20alpha-HSD mRNA is higher expressed in the ovary at pre-ovulation than in the placenta and oviduct at pre-parturition

Macaca fascicularis

-

725822

human immunodeficiency virus-infected pregnant women receiving protease inhibitor-based combination antiretroviral therapy show an elevation in placental expression of the 20-alpha-hydroxysteroid dehydrogenase

-

738806

mRNA levels increase gradually throughout the estrous cycle, the highest being in the corpus luteum (CL) 1 stage

-

726471

no difference in endometrial enzyme expression is detected between induced short and normal cycles in dairy cows treated with prostaglandin F2alpha and gonadotrophin-releasing hormone 24 h apart

-

741454

placental enzyme expression is significantly elevated in human immunodeficiency virus-infected pregnant women exposed to protease inhibitor-based combination antiretroviral therapy

-

738806

the amount of enzyme mRNA in cultured luteal cells increases with time and by treatment with the luteolysis agent prostaglandin F2alpha

D0VDT4

737453

the enzyme expression is upregulated by daunorubicin treament

739864

the protein is detected in the testes 1 week after birth and increases dramatically at 8 weeks

Q8K023

760948

6 M guanidine hydrochloride, 20 mM Tris-HCl buffer, pH 7.4, 100 mM KCl, 10% glycerol, 1 mM DTT, 1 h, 25°C

-

analysis

biotechnology

environmental protection

-

diesel exhaust components are inhibitory on 20alpha-hydroxysteroid dehydrogenase in liver and lung cytosol, with little inhibition in kidney cytosol

688031

food industry

medicine