Any feedback?
Information on EC 1.1.1.118 - glucose 1-dehydrogenase (NAD+)
for references in articles please use BRENDA:EC1.1.1.118Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.1.1.118 glucose 1-dehydrogenase (NAD+)Specify your search results
Word Map
- 1.1.1.118
- gluconate
-
electrode
- quinone
-
biosensors
-
pyrroloquinoline
-
electrochemical
-
biocatalyst
-
biofuel
- pqq
- megaterium
-
biocatalytic
-
enantiomeric
-
nanotube
-
anode
-
quinoproteins
-
amperometric
-
cathode
-
entner-doudoroff
- calcoaceticus
-
pqq-dependent
-
fad-dependent
- acidophilum
-
bioanode
- gluconobacter
- 2-ketogluconate
-
space-time
-
thermoplasma
-
biocathode
-
phosphate-solubilizing
-
bioreduction
- oxydans
-
self-powered
-
bioelectrochemical
-
bioelectrocatalytic
- sibiricum
-
exiguobacterium
-
electrocatalysts
-
glucometer
-
quinone-dependent
-
forespore
-
dipicolinic
- biotechnology
- synthesis
The enzyme appears in viruses and cellular organisms
Synonyms
glucose dehydrogenase, glucose 1-dehydrogenase, sygdh, d-aldohexose dehydrogenase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D-aldohexose dehydrogenase
-
-
-
-
D-glucose:NAD oxidoreductase
-
-
-
-
dehydrogenase, glucose (nicotinamide adenine dinucleotide)
-
-
-
-
GDH
glucose dehydrogenase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-glucose + NAD+ = D-glucono-1,5-lactone + NADH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
D-glucose:NAD+ 1-oxidoreductase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
37250-49-0
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-amino-2-deoxy-D-glucose + NAD+
? + NADH
-
26% activity compared to D-glucose in combination with NAD+
-
-
?
2-amino-2-deoxy-D-glucose + NADP+
? + NADPH
-
5% activity compared to D-glucose in combination with NAD+
-
-
?
2-deoxy-D-glucose + NADP+
? + NADPH
-
25% activity compared to D-glucose in combination with NAD+
-
-
?
3,6-dideoxy-D-galactose + NAD+
3,6-dideoxy-D-galactono-1,5-lactone + NADH
-
-
-
-
?
6-deoxy-D-glucose + NADP+
? + NADPH
-
9% activity compared to D-glucose in combination with NAD+
-
-
?
beta-D-galactose + NAD+
D-galactono-1,5-lactone + NADH
AHI17928.1
-
-
-
?
D-altrose + NAD+
D-altrono-1,5-lactone + NADH + H+
-
5% activity compared to D-glucose in combination with NAD+
-
-
?
D-altrose + NADP+
? + NADPH
-
12% activity compared to D-glucose in combination with NAD+
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH + H+
-
15% activity compared to D-glucose in combination with NAD+
-
-
?
D-gulose + NAD+
? + NADH
-
8% activity compared to D-glucose in combination with NAD+
-
-
?
D-gulose + NADP+
? + NADPH
-
12% activity compared to D-glucose in combination with NAD+
-
-
?
D-idose + NAD+
? + NADH
-
65% activity compared to D-glucose in combination with NAD+
-
-
?
D-idose + NADP+
? + NADPH
-
12% activity compared to D-glucose in combination with NAD+
-
-
?
D-mannose + NADP+
? + NADPH
-
4% activity compared to D-glucose in combination with NAD+
-
-
?
D-ribose + NADP+
? + NADPH
-
4% activity compared to D-glucose in combination with NAD+
-
-
?
D-xylose + NAD+
? + NADH
-
26% activity compared to D-glucose in combination with NAD+
-
-
?
D-xylose + NADP+
? + NADPH
-
28% activity compared to D-glucose in combination with NAD+
-
-
?
2-deoxy-D-glucose + NAD+
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
-
?
2-deoxy-D-glucose + NAD+
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
12% activity compared to D-glucose in combination with NAD+
-
-
?
2-deoxy-D-glucose + NAD+
2-deoxy-D-glucono-1,5-lactone + NADH + H+
Saccharolobus solfataricus MT-4 / DSM 5833
-
12% activity compared to D-glucose in combination with NAD+
-
-
?
6-deoxy-D-glucose + NAD+
6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
66% activity compared to D-glucose in combination with NAD+
-
-
?
6-deoxy-D-glucose + NAD+
6-deoxy-D-glucono-1,5-lactone + NADH + H+
Saccharolobus solfataricus MT-4 / DSM 5833
-
66% activity compared to D-glucose in combination with NAD+
-
-
?
D-glucose + NAD+
D-glucono-1,5-lactone + NADH + H+
-
100% activity
-
-
?
D-glucose + NAD+
D-glucono-1,5-lactone + NADH + H+
Saccharolobus solfataricus MT-4 / DSM 5833
-
100% activity
-
-
?
D-glucose + NAD+
D-glucono-1,5-lactone + NADH + H+
AHI17928.1
the enzyme prefers NADP+ as coenzyme to NAD+
-
-
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
-
9% activity compared to D-glucose in combination with NAD+
-
-
?
D-glucose + NADP+
D-glucono-1,5-lactone + NADPH + H+
Saccharolobus solfataricus MT-4 / DSM 5833
-
9% activity compared to D-glucose in combination with NAD+
-
-
?
additional information
?
-
-
no activity with: D-glucuronic acid, D-galacturonic acid, D-galactose 6-phosphate, D-glucose 6-phosphate, D-glucosamine, N-acetyl-D-glucosamine, 6-deoxy-D-allose, 6-iodo-6-deoxy-D-galactose, 2-acetamide-6-deoxy-D-allose, 2-acetamido-6-deoxy-D-altrose
-
-
?
additional information
?
-
-
no activity with: D-glucuronic acid, D-galacturonic acid, D-galactose 6-phosphate, D-glucose 6-phosphate, D-glucosamine, N-acetyl-D-glucosamine, 6-deoxy-D-allose, 6-iodo-6-deoxy-D-galactose, 2-acetamide-6-deoxy-D-allose, 2-acetamido-6-deoxy-D-altrose
-
-
?
additional information
?
-
-
no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
-
-
?
additional information
?
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
-
-
?
additional information
?
-
AHI17928.1
the enzyme shows no activity towards D-xylose, D-mannose, D-maltose, glucose 6-phosphate and sucrose
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NADPH
-
inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+
additional information
-
D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+
-
EDTA
-
5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Acrodynia
Identification and characterization of a Pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple.
Dehydration
Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase.
Dehydration
The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus.
Galactosemias
Erroneous glucose recordings while using mutant variant of quinoprotein glucose dehydrogenase glucometer in a child with galactosemia.
glucose 1-dehydrogenase (nad+) deficiency
[Phosphate glucose dehydrogenase deficiency causing hyperbilirubinemia in the newborn]
Malaria
Analysis of the genetic variants of glucose-6-phosphate dehydrogenase in inhabitants of the 4th Nile cataract region in Sudan.
Photosensitivity Disorders
Biohydrogen production from sucrose using the visible light sensitization of artificial Zn chlorophyll-a.
Photosensitivity Disorders
Visible light induced biohydrogen production from sucrose using the photosensitization of Mg chlorophyll-a.
Photosensitivity Disorders
Visible light-induced H2 production from cellulose using photosensitization of Mg chlorophyll a.
Starvation
Glucose dehydrogenase, glucose-6-phosphate dehydrogenase and hexokinase in liver of rainbow trout (Salmo gairdneri). Effects of starvation and temperature variations.
Starvation
[Variations of enzymatic activities in an asporogenic mutant of Bacillus megaterium for various nutritional deficiencies]
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
22
D-galactose
-
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
0.44
D-glucose
-
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
8
D-glucose
-
in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
2.2
D-xylose
-
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
68
D-xylose
-
in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
1.2
NAD+
-
in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
1.2
NAD+
-
in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
0.03
NADP+
-
in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
0.03
NADP+
-
in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
0.03
NADP+
-
in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 8
AHI17928.1
pH 5.5: about 50% of maximal activity, pH 8.0: about 40% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10 - 60
AHI17928.1
10°C: about 35% of maximal activity, 20°C: about 80% of maximal activity, 60°C: about 75% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
a codon-optimized gene Sygdh encoding glucose 1-dehydrogenase (SyGDH) is synthesized based on the putative GDH gene sequence (Ta0897) in Thermoplasma acidophilum genomic DNA, and expressed in Escherichia coli BL21(DE3)
AHI17928.1
GenBank
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q9HK51_THEAC
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
255
0
27873
TrEMBL
other Location (Reliability: 5)
Q97CM7_THEVO
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
261
0
28195
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30100
-
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS
60200
-
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
homotetramer
homodimer
-
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride
homodimer
Saccharolobus solfataricus MT-4 / DSM 5833
-
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride
-
homotetramer
-
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS
homotetramer
Saccharolobus solfataricus MT-4 / DSM 5833
-
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E170K
-
has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration
E170K/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
P45A/F155Y/V227A
-
has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
E170K
-
has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration
-
E170K/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
-
P45A/F155Y/V227A
-
has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants
-
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9
-
the enzyme stability does not vary significantly in the pH range 5-9
639110
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acetone
DMSO
AHI17928.1
retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h
Ethanol