Information on EC 1.1.1.118 - glucose 1-dehydrogenase (NAD+)

additional information

Substrates: no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
Products: -

additional information

AHI17928.1

Substrates: the enzyme shows no activity towards D-xylose, D-mannose, D-maltose, glucose 6-phosphate and sucrose
Products: -

show all columns Go to Natural Substrate Search

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

3 entries

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

Substrates: -
Products: -

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

Substrates: -
Products: -

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

Substrates: -
Products: -

show all columns Go to Cofactor Search

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NAD+

NADP+

NAD+

cofactor

NAD+

cofactor

NAD+

NAD+

NAD+

NAD+

AHI17928.1

the enzyme prefers NADP+ as coenzyme to NAD+

NADP+

NADP+

AHI17928.1

the enzyme prefers NADP+ as coenzyme to NAD+

show all columns Go to Metals and Ions Search

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ca2+

maximally active at 20 mM

Mg2+

maximally active at 20 mM

Mn2+

maximally active at 20 mM

Zn2+

AHI17928.1

additional information

Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation

show all columns Go to Inhibitor Search

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-mercaptoethanol

rapid inactivation at 1 mM

5,5'-dithiobis-(2-nitrobenzoic acid)

Cu2+

AHI17928.1

EDTA

Guanidinium chloride

rapid inactivation at 4 M

N-ethylmaleimide

NADPH

inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+

tetrahydrofuran

additional information

D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+

EDTA

5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity

EDTA

AHI17928.1

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DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

Acrodynia

Identification and characterization of a Pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple.

8979341

Dehydration

Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase.

12824170

Dehydration

The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus.

15265860

Galactosemias

Erroneous glucose recordings while using mutant variant of quinoprotein glucose dehydrogenase glucometer in a child with galactosemia.

24251189

glucose 1-dehydrogenase (nad+) deficiency

[Phosphate glucose dehydrogenase deficiency causing hyperbilirubinemia in the newborn]

5789870

Malaria

Analysis of the genetic variants of glucose-6-phosphate dehydrogenase in inhabitants of the 4th Nile cataract region in Sudan.

23146719

Pancreatitis

Peritoneal dialysis: a primary care perspective.

16809653

Photosensitivity Disorders

Biohydrogen production from sucrose using the visible light sensitization of artificial Zn chlorophyll-a.

12526718

Photosensitivity Disorders

Photoinduced biohydrogen production from biomass.

19325796

Photosensitivity Disorders

Visible light induced biohydrogen production from sucrose using the photosensitization of Mg chlorophyll-a.

12121148

Photosensitivity Disorders

Visible light-induced H2 production from cellulose using photosensitization of Mg chlorophyll a.

100149770

Starvation

Glucose dehydrogenase, glucose-6-phosphate dehydrogenase and hexokinase in liver of rainbow trout (Salmo gairdneri). Effects of starvation and temperature variations.

1769217

Starvation

[Variations of enzymatic activities in an asporogenic mutant of Bacillus megaterium for various nutritional deficiencies]

826332

show all columns Go to KM Value Search

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5.8

D-fucose

D-galactose

in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.44 - 8

D-glucose

2.2 - 68

D-xylose

0.67 - 80

NAD+

0.03 - 157.9

NADP+

0.44

D-glucose

in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.86

D-glucose

7.48

D-glucose

AHI17928.1

pH 7.5, 40°C

D-glucose

in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

2.2

D-xylose

in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

D-xylose

in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.67

NAD+

AHI17928.1

pH 7.5, 40°C

1.2

NAD+

in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

1.2

NAD+

in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

NAD+

0.03

NADP+

in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.03

NADP+

in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.03

NADP+

in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

157.9

NADP+

AHI17928.1

pH 7.5, 40°C

show all columns Go to Turnover Number Search

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

64.9

D-glucose

AHI17928.1

pH 7.5, 40°C

69.7

NAD+

AHI17928.1

pH 7.5, 40°C

101.9

NADP+

AHI17928.1

pH 7.5, 40°C

show all columns Go to kcat/KM Value Search

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

8.67

D-glucose

AHI17928.1

pH 7.5, 40°C

104

NAD+

AHI17928.1

pH 7.5, 40°C

0.64

NADP+

AHI17928.1

pH 7.5, 40°C

show all columns Go to Ki Value Search

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Ki VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.075

NADPH

using D-glucose as a substrate

show all columns Go to IC50 Value Search

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IC50 VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5,5'-dithiobis-(2-nitrobenzoic acid)

Saccharolobus solfataricus

at 25°C and pH 9

N-ethylmaleimide

Saccharolobus solfataricus

at 25°C and pH 9

show all columns Go to Specific Activity Search

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.7

unpurified enzyme, at 70°C

437

after 623fold purification, at 70°C

8.5

AHI17928.1

40°C, pH 7.5, coenzyme: NAD+

86.3

AHI17928.1

40°C, pH 7.5, coenzyme: NADP+

show all columns Go to pH Optimum Search

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10 - 10.2

Corynebacterium sp.

assay at

7.5

AHI17928.1

show all columns Go to pH Range Search

8 - 8.5

Tris-HCl buffer

9 - 10

glycine-NaOH buffer

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5.5 - 8

AHI17928.1

pH 5.5: about 50% of maximal activity, pH 8.0: about 40% of maximal activity

show all columns Go to Temperature Optimum Search

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

assay at

AHI17928.1

show all columns Go to Temperature Range Search

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10 - 60

AHI17928.1

10°C: about 35% of maximal activity, 20°C: about 80% of maximal activity, 60°C: about 75% of maximal activity

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

701045, 739713

brenda

701045, 739713

brenda

Corynebacterium sp.

No. 93-1

Corynebacterium sp. No. 93-1

brenda

No. 93-1

brenda

i.e. Acetobacter xylinum

brenda

Pseudomonas putida

IAM 12014

Pseudomonas putida IAM 12014

brenda

IAM 12014

brenda

Pseudomonas sp. MSU-1

MSU-1

brenda

brenda

Saccharolobus solfataricus MT-4 / DSM 5833

brenda

a codon-optimized gene Sygdh encoding glucose 1-dehydrogenase (SyGDH) is synthesized based on the putative GDH gene sequence (Ta0897) in Thermoplasma acidophilum genomic DNA, and expressed in Escherichia coli BL21(DE3)

AHI17928.1

GenBank

brenda

brenda

MSU-1

brenda

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

Q9HK51 pBLAST

Q9HK51_THEAC

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

255

27873

TrEMBL

Show Sequence

Q97CM7 pBLAST

Q97CM7_THEVO

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)

261

28195

TrEMBL

Show Sequence

other Location (Reliability: 2)

Go to PDB and Structure Links Search

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PDB

SCOP

CATH

UNIPROT

ORGANISM

Thermoplasma volcanium GSS1

show all columns Go to Molecular Weight Search

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

124000

gel filtration

30000

x * 30000, SDS-PAGE

30100

4 * 30100, gel filtration, in the presence of 5% (w/v) SDS

60200

2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride

show all columns Go to Subunit Search

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

homodimer

homotetramer

x * 30000, SDS-PAGE

x * 30000, SDS-PAGE

homodimer

2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride

homodimer

Saccharolobus solfataricus MT-4 / DSM 5833

2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride

homotetramer

4 * 30100, gel filtration, in the presence of 5% (w/v) SDS

homotetramer

Saccharolobus solfataricus MT-4 / DSM 5833

4 * 30100, gel filtration, in the presence of 5% (w/v) SDS

show all columns Go to Protein Variants Search

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E170K

has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration

E170K/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

P45A/F155Y/V227A

has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants

P45A/N46E/F155Y/E170K/V227A/W230F/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

E170K

has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration

E170K/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

P45A/F155Y/V227A

P45A/N46E/F155Y/E170K/V227A/W230F/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

show all columns Go to pH Stability Search

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5 - 9

show all columns Go to Temperature Stability Search

the enzyme stability does not vary significantly in the pH range 5-9

6 - 8

30°C, 30 min, stable

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Corynebacterium sp.

pH 7.0, 10 min, stable up to

show all columns Go to Organic Solvent Search

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Acetone

DMSO

AHI17928.1

retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h

Ethanol

isopropanol

AHI17928.1

retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h

Methanol

3 entries

N,N-dimethylformamide

AHI17928.1

half-life of 3 h

SDS

a slow time-dependent decrease of activity is observed in the presence of 0.1% SDS

urea

a slow time-dependent decrease of activity is observed in the presence of 4 M urea

additional information

2 M urea and 0.05% (w/v) SDS do not cause any loss of enzymic activity

Acetone

stable in the presence of chaotropic agents and water-miscible organic solvents such as acetone

Acetone

Saccharolobus solfataricus MT-4 / DSM 5833

stable in the presence of chaotropic agents and water-miscible organic solvents such as acetone

Ethanol

a slow time-dependent decrease of activity is observed in the presence of ethanol

Ethanol

AHI17928.1

retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h

Methanol

stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol

Methanol

Saccharolobus solfataricus MT-4 / DSM 5833

stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol

Methanol

AHI17928.1

retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h

Go to Storage Stability Search

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

37°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 40 days, 50% loss of activity

4°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, several months, remains stable

70°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 45 h, 50% loss of activity

stable to freezing for at least 6 months, pH 7.0, 10 mM sodium phosphate buffer

Go to Purification (commentary) Search

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

affinity chromatographys

AHI17928.1

His-tag GDH variants

SP-Sephadex C-50 gel filtration, phenyl-Sepharose column chromatography, and Affi-Gel Blue matrix gel filtration

Go to Cloned (commentary) Search

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

AHI17928.1

His-tag GDH variants, into pET28a and expressed in Escherichia coli BL21(DE3)

show all columns Go to Application Search

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

biotechnology

immobilization of GDH1 on the surface of a graphite felt electrode, construction and optimization of an electrochemical bioreactor, co-immobilization of 3,4-dihydroxybenzaldehyde as mediator allows the system to operate at 0.2 V and increases both the activity (2.4-times) and the stability of the immobilized enzyme by 2.2-times, the immobilized enzyme is termed IMGDH1

synthesis

additional information

AHI17928.1

application in NADPH regeneration for the asymmetric reduction of haloketone by a carbonyl reductase in organic solvent/buffer system

synthesis

Pseudomonas putida

glucose dehydrogenase and L-carnitine dehydrogenase are coimmobilized in a nanofiltration membrane bioreactor for the continuous production of 1-carnitine from 3-dehydrocarnitine with NADH regeneration

Pseudomonas putida IAM 12014

synthesis

top print hide References Search

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

285776

Hu, A.S.L.; Cline, A.L.

The regulation of some sugar dehydrogenases in a Pseudomonad

Biochim. Biophys. Acta

237-245

1964

Pseudomonas sp.

14251301

brenda

Kobayashi, Y.; Horikoshi, K.

Identification and growth characteristics of alkalophilic Corynebacterium sp. which produces NAD(P)-dependent maltose dehydrogenase and glucose dehydrogenase

Agric. Biol. Chem.

41-47

1980

Corynebacterium sp., Corynebacterium sp. No. 93-1

brenda

Anderson, R.L.; Dahms, A.S.

D-Aldohexose dehydrogenase

Methods Enzymol.

147-150

1975

Pseudomonas sp., Pseudomonas sp. MSU-1

236432

brenda

Lin, S.S.; Miyawaki, O.; Nakamura, K.

Continuous production of L-carnitine with NADH regeneration by a nanofiltration membrane reactor with coimmobilized L-carnitine dehydrogenase and glucose dehydrogenase

J. Biosci. Bioeng.

361-364

1999

Pseudomonas putida, Pseudomonas putida IAM 12014

16232482

brenda

Giardina, P.; DeBasia, M.G.; DeRosa, M.; Gambacort, A.; Buonocore, V.

Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus

Biochem. J.

239

517-522

1986

Saccharolobus solfataricus, Saccharolobus solfataricus MT-4 / DSM 5833

3827812

brenda

Manjon, A.; Obon, J.M.; Casanova, P.; Fernandez, V.M.; Ilborra1, J.L.

Increased activity of glucose dehydrogenase co-immobilized with a redox mediator in a bioreactor with electrochemical NAD+ regeneration

Biotechnol. Lett.

1227-1232

2002

Komagataeibacter xylinus

brenda

Vazquez-Figueroa, E.; Yeh, V.; Broering, J.; Chaparro-Riggers, J.; Bommarius, A.

Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media

Protein Eng. Des. Sel.

673-680

2008

Bacillus subtilis, Bacillus subtilis 168

18799474

brenda

Zhang, X.; Rao, Z.; Zhang, L.; Xu, M.; Yang, T.

Efficient 9alpha-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9alpha-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration

SpringerPlus

1207

2016

Bacillus subtilis, Bacillus subtilis 168

27516945

brenda