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EC 1.14.19.18 Details
show Brenda entryEC number
1.14.19.18
Accepted name
sphingolipid 8-(E)-desaturase
Reaction
a (4E)-sphing-4-enine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4E,8E)-sphing-4,8-dienine ceramide + 2 ferricytochrome b5 + 2 H2O
Other name(s)
8-sphingolipid desaturase (ambiguous),
8 fatty acid desaturase (ambiguous),
DELTA8-sphingolipid desaturase (ambiguous)
Systematic name
(4E)-sphing-4-enine ceramide,ferrocytochrome b5:oxygen oxidoreductase (8,9-trans dehydrogenating)
Comment
The enzyme, characterized from the yeasts Kluyveromyces lactis and Candida albicans [1] and from the diatom Thalassiosira pseudonana [2], introduces a trans double bond at the 8-position of sphingoid bases in sphingolipids. The enzyme determines the position of the double bond by its distance from the alcohol end of the sphingoid base, and contains a cytochrome b5 domain that acts as the direct electron donor to the active site of the desaturase [3]. The homologous enzymes from higher plants, EC 1.14.19.29, sphingolipid 8-(E/Z)-desaturase, act on phytosphinganine (4-hydroxysphinganine) and produces a mixture of trans and cis isomers.
History
created 2015
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.4.99.1 D-amino-acid dehydrogenase
