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EC 1.14.99.38 Details
show Brenda entryEC number
1.14.99.38
Accepted name
cholesterol 25-monooxygenase
Reaction
cholesterol + reduced acceptor + O2 = 25-hydroxycholesterol + acceptor + H2O
Other name(s)
cholesterol 25-hydroxylase (ambiguous)
Systematic name
cholesterol,hydrogen-donor:oxygen oxidoreductase (25-hydroxylating)
CAS registry number
60202-07-5
Comment
Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P-450. Instead, it uses diiron cofactors to catalyse the hydroxylation of hydrophobic substrates [1]. The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues [4,5]. In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs). cf. EC 1.17.99.10, cholesterol C-25 hydroxylase.
History
created 2005, modified 2020
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.99.5 created 1972, modified 1986, modified 2000, deleted 2000 1.14.99.6 created 1972, modified 2000, deleted 2000 1.14.99.8 created 1972, deleted 1984 1.14.99.13 created 1972, deleted 1984 1.14.99.16 created 1972, deleted 2002 1.14.99.17 created 1972, deleted 1976 1.14.99.18 created 1976, modified 1999, deleted 2003 1.14.99.25 created 1986, deleted 2000
