EC Explorer
[browse EC tree] - [search]
EC 1.1.99.36 Details
show Brenda entryEC number
1.1.99.36
Accepted name
alcohol dehydrogenase (nicotinoprotein)
Reaction
ethanol + acceptor = acetaldehyde + reduced acceptor
Other name(s)
NDMA-dependent alcohol dehydrogenase,
nicotinoprotein alcohol dehydrogenase,
np-ADH,
ethanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase
Systematic name
ethanol:acceptor oxidoreductase
Comment
Contains Zn2+. Nicotinoprotein alcohol dehydrogenases are unique medium-chain dehydrogenases/reductases (MDR) alcohol dehydrogenases that have a tightly bound NAD+/NADH cofactor that does not dissociate during the catalytic process. Instead, the cofactor is regenerated by a second substrate or electron carrier. While the in vivo electron acceptor is not known, N,N-dimethyl-4-nitrosoaniline (NDMA), which is reduced to 4-(hydroxylamino)-N,N-dimethylaniline, can serve this function in vitro.
The enzyme from the Gram-positive bacterium Amycolatopsis methanolica can accept many primary alcohols as substrates, including benzylalcohol [1].
History
created 2010
back to topEC Tree
1 Oxidoreductases
1.1.1 With NAD+ or NADP+ as acceptor
1.1.99.15 created 1978, deleted 1980 1.1.99.17 created 1982, deleted 2003
