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EC 6.3.1.14 Details
show Brenda entryEC number
6.3.1.14
Accepted name
diphthine—ammonia ligase
Reaction
ATP + diphthine-[translation elongation factor 2] + NH3 = AMP + diphosphate + diphthamide-[translation elongation factor 2]
Other name(s)
diphthamide synthase,
diphthamide synthetase,
DPH6 (gene name),
ATPBD4 (gene name),
diphthine:ammonia ligase (AMP-forming)
Systematic name
diphthine-[translation elongation factor 2]:ammonia ligase (AMP-forming)
CAS registry number
114514-33-9
Comment
This amidase catalyses the last step in the conversion of an L-histidine residue in the translation elongation factor EF2 to diphthamide. This factor is found in all archaea and eukaryota, but not in eubacteria, and is the target of bacterial toxins such as the diphtheria toxin and the Pseudomonas exotoxin A (see EC 2.4.2.36, NAD+—diphthamide ADP-ribosyltransferase). The substrate of the enzyme, diphthine, is produced by EC 2.1.1.98, diphthine synthase.
History
created 1990 as EC 6.3.2.22, transferred 2010 to EC 6.3.1.14, modified 2013
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1 Oxidoreductases
1.1.1 With NAD+ or NADP+ as acceptor
1.1.99.15 created 1978, deleted 1980 1.1.99.17 created 1982, deleted 2003
