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EC 4.6.1.24 Details
EC number
4.6.1.24
Accepted name
ribonuclease T1
Reaction
[RNA] containing guanosine + H2O = an [RNA fragment]-3′-guanosine-3′-phosphate + a 5′-hydroxy-ribonucleotide-3′-[RNA fragment] (overall reaction);;(1a) [RNA] containing guanosine = [RNA fragment]-3′-guanosine-2′,3′-cyclophosphate + a 5′-hydroxy-ribonucleotide-3′-[RNA fragment];; (1b) [RNA fragment]-3′-guanosine-2′,3′-cyclophosphate + H2O = [RNA fragment]-3′-guanosine-3′-phosphate
Other name(s)
barnase, bacterial ribonuclease Sa, guanyloribonuclease, Aspergillus oryzae ribonuclease, RNase N1, RNase N2, ribonuclease N3, ribonuclease U1, ribonuclease F1, ribonuclease Ch, ribonuclease PP1, ribonuclease SA, RNase F1, ribonuclease C2, binase, RNase Sa, guanyl-specific RNase, RNase G, RNase T1, ribonuclease guaninenucleotido-2′-transferase (cyclizing), ribonuclease N1
Systematic name
[RNA]-guanosine 5′-hydroxy-ribonucleotide-3′-[RNA fragment]-lyase (cyclicizing; [RNA fragment]-3′-guanosine-2′,3′-cyclophosphate-forming and hydrolysing)
Comment
A family of related enzymes found in some fungi and bacteria. The enzyme is specific for cleavage at the 3′-phosphate group of guanosine in single stranded RNA, and catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5′-hydroxy-phosphooligonucletides and 3′-phosphooligonucleotides ending in Gp with 2′,3′-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3′,5′-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5′-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3′-nucleotides.
History
created 1961 as EC 3.1.4.8, transferred 1965 to EC 2.7.7.26, reinstated 1972 as EC 3.1.4.8, transferred 1978 to EC 3.1.27.3, transferred 2020 to EC 4.6.1.24
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