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EC 1.17.99.10 Details

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EC number

1.17.99.10

Accepted name

steroid C-25 hydroxylase

Reaction

cholest-4-en-3-one + acceptor + H₂O = 25-hydroxycholest-4-en-3-one + reduced acceptor

Other name(s)

s25dA1 (gene name), s25dA1B3 (gene name), s25dA1C3 (gene name), cholesterol C-25 dehydrogenase, steroid C-25 dehydrogenase

Systematic name

cholest-4-en-3-one:acceptor oxidoreductase (25-hydroxylating)

Comment

The enzyme, characterized from the bacterium Sterolibacterium denitrificans, participates in the anaerobic degradation of cholesterol. The enzyme can accept several substrates including vitamin D₃. The enzyme contains a bis(guanylyl molybdopterin) cofactor, five [Fe-S] clusters, and one heme b. cf. EC 1.14.99.38, cholesterol 25-monooxygenase, an oxygen-requiring eukaryotic enzyme that catalyses a similar transformation.

History

created 2020

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.6.1 With NAD⁺ or NADP⁺ as acceptor

1.6.2 With a heme protein as acceptor

1.6.3 With oxygen as acceptor

1.6.4 With a disulfide as acceptor (deleted sub-subclass)

1.6.5 With a quinone or similar compound as acceptor

1.6.6 With a nitrogenous group as acceptor

1.6.7 With an iron-sulfur protein as acceptor (deleted sub-subclass)

1.6.8 With a flavin as acceptor (deleted sub-subclass)

1.6.99 With unknown physiological acceptors

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.17.1 With NAD⁺ or NADP⁺ as acceptor

1.17.2 With a cytochrome as acceptor

1.17.3 With oxygen as acceptor

1.17.4 With a disulfide as acceptor

1.17.5 With a quinone or similar compound as acceptor

1.17.7 With an iron-sulfur protein as acceptor

1.17.8 With a flavin as acceptor

1.17.9 With a copper protein as acceptor

1.17.98 With other, known, physiological acceptors

1.17.99 With unknown physiological acceptors

1.17.99.1 4-methylphenol dehydrogenase (hydroxylating)

1.17.99.2 ethylbenzene hydroxylase

1.17.99.3 3α,7α,12α-trihydroxy-5β-cholestanoyl-CoA 24-hydroxylase

1.17.99.4 uracil/thymine dehydrogenase

1.17.99.5 bile-acid 7α-dehydroxylase

1.17.99.6 epoxyqueuosine reductase

1.17.99.7 formate dehydrogenase (acceptor)

1.17.99.8 limonene dehydrogenase

1.17.99.9 heme a synthase

1.17.99.10 steroid C-25 hydroxylase

1.17.99.11 3-oxo-Δ¹-steroid hydratase/dehydrogenase

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.20.1 With NAD⁺ or NADP⁺ as acceptor

1.20.2 With a cytochrome as acceptor

1.20.4 With disulfide as acceptor

1.20.9 With a copper protein as acceptor

1.20.98 With other, known, physiological acceptors

1.20.99 With unknown physiological acceptors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)