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EC 2.3.2.26 Details

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EC number

2.3.2.26

Accepted name

HECT-type E3 ubiquitin transferase

Reaction

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N⁶-ubiquitinyl-L-lysine (overall reaction);;(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine;;(1b) [HECT-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [HECT-type E3 ubiquitin transferase]-L-cysteine + [acceptor protein]-N⁶-ubiquitinyl-L-lysine

Other name(s)

HECT E3 ligase (misleading), ubiquitin transferase HECT-E3, S-ubiquitinyl-[HECT-type E3-ubiquitin transferase]-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming)

Systematic name

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein] ubiquitin transferase (isopeptide bond-forming)

Comment

In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the ε-amino group of an L-lysine residue of the acceptor protein. cf. EC 2.3.2.27, RING-type E3 ubiquitin transferase and EC 2.3.2.31, RBR-type E3 ubiquitin transferase.

History

created 2015, modified 2017

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.17.1 With NAD⁺ or NADP⁺ as acceptor

1.17.2 With a cytochrome as acceptor

1.17.3 With oxygen as acceptor

1.17.4 With a disulfide as acceptor

1.17.5 With a quinone or similar compound as acceptor

1.17.7 With an iron-sulfur protein as acceptor

1.17.8 With a flavin as acceptor

1.17.9 With a copper protein as acceptor

1.17.98 With other, known, physiological acceptors

1.17.99 With unknown physiological acceptors

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.20.1 With NAD⁺ or NADP⁺ as acceptor

1.20.2 With a cytochrome as acceptor

1.20.4 With disulfide as acceptor

1.20.9 With a copper protein as acceptor

1.20.98 With other, known, physiological acceptors

1.20.99 With unknown physiological acceptors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

2.1 Transferring one-carbon groups

2.2 Transferring aldehyde or ketonic groups

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.2 Aminoacyltransferases

2.3.2.1 D-glutamyltransferase

2.3.2.2 γ-glutamyltransferase

2.3.2.3 lysyltransferase

2.3.2.4 γ-glutamylcyclotransferase

2.3.2.5 glutaminyl-peptide cyclotransferase

2.3.2.6 lysine/arginine leucyltransferase

2.3.2.7 aspartyltransferase

2.3.2.8 arginyltransferase

2.3.2.9 agaritine γ-glutamyltransferase

2.3.2.10 UDP-N-acetylmuramoylpentapeptide-lysine N⁶-alanyltransferase

2.3.2.11 alanylphosphatidylglycerol synthase

2.3.2.12 peptidyltransferase

2.3.2.13 protein-glutamine γ-glutamyltransferase

2.3.2.14 D-alanine γ-glutamyltransferase

2.3.2.15 glutathione γ-glutamylcysteinyltransferase

2.3.2.16 lipid II:glycine glycyltransferase

2.3.2.17 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase

2.3.2.18 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase

2.3.2.19 ribostamycin:4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoate transferase

2.3.2.20 cyclo(L-leucyl-L-phenylalanyl) synthase

2.3.2.21 cyclo(L-tyrosyl-L-tyrosyl) synthase

2.3.2.22 cyclo(L-leucyl-L-leucyl) synthase

2.3.2.23 E2 ubiquitin-conjugating enzyme

2.3.2.24 (E3-independent) E2 ubiquitin-conjugating enzyme

2.3.2.25 N-terminal E2 ubiquitin-conjugating enzyme

2.3.2.26 HECT-type E3 ubiquitin transferase

2.3.2.27 RING-type E3 ubiquitin transferase

2.3.2.28 L-allo-isoleucyltransferase

2.3.2.29 aspartate/glutamate leucyltransferase

2.3.2.30 L-ornithine N^α-acyltransferase

2.3.2.31 RBR-type E3 ubiquitin transferase

2.3.2.32 cullin-RING-type E3 NEDD8 transferase

2.3.2.33 RCR-type E3 ubiquitin transferase

2.3.2.34 E2 NEDD8-conjugating enzyme

2.3.2.35 capsaicin synthase

2.3.2.36 RING-type E3 ubiquitin transferase (cysteine targeting)

2.3.3 Acyl groups converted into alkyl groups on transfer

2.4 Glycosyltransferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.6 Transferring nitrogenous groups

2.7 Transferring phosphorus-containing groups

2.8 Transferring sulfur-containing groups

2.9 Transferring selenium-containing groups

2.10 Transferring molybdenum- or tungsten-containing groups

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Release 2023.1 (January 2023)