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EC 2.3.2.25 Details

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EC number

2.3.2.25

Accepted name

N-terminal E2 ubiquitin-conjugating enzyme

Reaction

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein] (overall reaction);;(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[N-terminal ubiquitin-conjugating enzyme]-L-cysteine;;(1b) S-ubiquitinyl-[N-terminal E2 ubiquitin-conjugating E2 enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine + N-ubiquitinyl-[acceptor protein]-N-terminal amino acid

Other name(s)

Ube2w, N-terminal ubiquitin-conjugating enzyme E2

Systematic name

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine:acceptor protein ubiquitin ligase (peptide bond-forming)

Comment

The enzyme ubiquitinylates the N-terminus of the acceptor protein. It is not reactive towards free lysine.

History

created 2015

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.17.1 With NAD⁺ or NADP⁺ as acceptor

1.17.2 With a cytochrome as acceptor

1.17.3 With oxygen as acceptor

1.17.4 With a disulfide as acceptor

1.17.5 With a quinone or similar compound as acceptor

1.17.7 With an iron-sulfur protein as acceptor

1.17.8 With a flavin as acceptor

1.17.9 With a copper protein as acceptor

1.17.98 With other, known, physiological acceptors

1.17.99 With unknown physiological acceptors

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.20.1 With NAD⁺ or NADP⁺ as acceptor

1.20.2 With a cytochrome as acceptor

1.20.4 With disulfide as acceptor

1.20.9 With a copper protein as acceptor

1.20.98 With other, known, physiological acceptors

1.20.99 With unknown physiological acceptors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

2.1 Transferring one-carbon groups

2.2 Transferring aldehyde or ketonic groups

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.2 Aminoacyltransferases

2.3.2.1 D-glutamyltransferase

2.3.2.2 γ-glutamyltransferase

2.3.2.3 lysyltransferase

2.3.2.4 γ-glutamylcyclotransferase

2.3.2.5 glutaminyl-peptide cyclotransferase

2.3.2.6 lysine/arginine leucyltransferase

2.3.2.7 aspartyltransferase

2.3.2.8 arginyltransferase

2.3.2.9 agaritine γ-glutamyltransferase

2.3.2.10 UDP-N-acetylmuramoylpentapeptide-lysine N⁶-alanyltransferase

2.3.2.11 alanylphosphatidylglycerol synthase

2.3.2.12 peptidyltransferase

2.3.2.13 protein-glutamine γ-glutamyltransferase

2.3.2.14 D-alanine γ-glutamyltransferase

2.3.2.15 glutathione γ-glutamylcysteinyltransferase

2.3.2.16 lipid II:glycine glycyltransferase

2.3.2.17 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase

2.3.2.18 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase

2.3.2.19 ribostamycin:4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoate transferase

2.3.2.20 cyclo(L-leucyl-L-phenylalanyl) synthase

2.3.2.21 cyclo(L-tyrosyl-L-tyrosyl) synthase

2.3.2.22 cyclo(L-leucyl-L-leucyl) synthase

2.3.2.23 E2 ubiquitin-conjugating enzyme

2.3.2.24 (E3-independent) E2 ubiquitin-conjugating enzyme

2.3.2.25 N-terminal E2 ubiquitin-conjugating enzyme

2.3.2.26 HECT-type E3 ubiquitin transferase

2.3.2.27 RING-type E3 ubiquitin transferase

2.3.2.28 L-allo-isoleucyltransferase

2.3.2.29 aspartate/glutamate leucyltransferase

2.3.2.30 L-ornithine N^α-acyltransferase

2.3.2.31 RBR-type E3 ubiquitin transferase

2.3.2.32 cullin-RING-type E3 NEDD8 transferase

2.3.2.33 RCR-type E3 ubiquitin transferase

2.3.2.34 E2 NEDD8-conjugating enzyme

2.3.2.35 capsaicin synthase

2.3.2.36 RING-type E3 ubiquitin transferase (cysteine targeting)

2.3.3 Acyl groups converted into alkyl groups on transfer

2.4 Glycosyltransferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.6 Transferring nitrogenous groups

2.7 Transferring phosphorus-containing groups

2.8 Transferring sulfur-containing groups

2.9 Transferring selenium-containing groups

2.10 Transferring molybdenum- or tungsten-containing groups

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Release 2023.1 (January 2023)