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EC 2.3.2.24 Details
EC number
2.3.2.24
Accepted name
(E3-independent) E2 ubiquitin-conjugating enzyme
Reaction
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine (overall reaction);;(1a) [E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) ubiquitin-conjugating enzyme]-S-monoubiquitinyl-L-cysteine;;(1b) [(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine
Other name(s)
E2-230K, UBE2O, E3-independent ubiquitin-conjugating enzyme E2
Systematic name
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine:L-lysine ubiquitinyl transferase ([E3 ubiquitin transferase]-independent)
Comment
The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC 2.3.2.26, EC 2.3.2.27). It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg2+-ATP and catalyses the conjugation of ubiquitin to protein substrates, independently of E3. This transfer has only been observed with small proteins. In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L-lysine methyl ester) has been observed [1].
History
created 2015
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EC Tree
1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.2 Acting on the aldehyde or oxo group of donors
1.3 Acting on the CH-CH group of donors
1.4 Acting on the CH-NH2 group of donors
1.5 Acting on the CH-NH group of donors
1.6 Acting on NADH or NADPH
1.7 Acting on other nitrogenous compounds as donors
1.8 Acting on a sulfur group of donors
1.9 Acting on a heme group of donors
1.10 Acting on diphenols and related substances as donors
1.11 Acting on a peroxide as acceptor
1.12 Acting on hydrogen as donor
1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.15 Acting on superoxide as acceptor
1.16 Oxidizing metal ions
1.17 Acting on CH or CH2 groups
1.17.1 With NAD+ or NADP+ as acceptor
1.17.2 With a cytochrome as acceptor
1.17.3 With oxygen as acceptor
1.17.4 With a disulfide as acceptor
1.17.5 With a quinone or similar compound as acceptor
1.17.7 With an iron-sulfur protein as acceptor
1.17.8 With a flavin as acceptor
1.17.9 With a copper protein as acceptor
1.17.98 With other, known, physiological acceptors
1.17.99 With unknown physiological acceptors
1.18 Acting on iron-sulfur proteins as donors
1.19 Acting on reduced flavodoxin as donor
1.20 Acting on phosphorus or arsenic in donors
1.20.1 With NAD+ or NADP+ as acceptor
1.20.2 With a cytochrome as acceptor
1.20.4 With disulfide as acceptor
1.20.9 With a copper protein as acceptor
1.20.98 With other, known, physiological acceptors
1.20.99 With unknown physiological acceptors
1.21 Catalysing the reaction X-H + Y-H = X-Y
1.22 Acting on halogen in donors
1.23 Reducing C-O-C group as acceptor
1.97 Other oxidoreductases
1.98 Enzymes using H2 as reductant (deleted subclass)
1.99 Other enzymes using O2 as oxidant (deleted subclass)
2 Transferases
2.1 Transferring one-carbon groups
2.2 Transferring aldehyde or ketonic groups
2.3 Acyltransferases
2.3.1 Transferring groups other than aminoacyl groups
2.3.2 Aminoacyltransferases
2.3.2.1 D-glutamyltransferase
2.3.2.2 γ-glutamyltransferase
2.3.2.3 lysyltransferase
2.3.2.4 γ-glutamylcyclotransferase
2.3.2.5 glutaminyl-peptide cyclotransferase
2.3.2.6 lysine/arginine leucyltransferase
2.3.2.7 aspartyltransferase
2.3.2.8 arginyltransferase
2.3.2.9 agaritine γ-glutamyltransferase
2.3.2.10 UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase
2.3.2.11 alanylphosphatidylglycerol synthase
2.3.2.12 peptidyltransferase
2.3.2.13 protein-glutamine γ-glutamyltransferase
2.3.2.14 D-alanine γ-glutamyltransferase
2.3.2.15 glutathione γ-glutamylcysteinyltransferase
2.3.2.16 lipid II:glycine glycyltransferase
2.3.2.17 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
2.3.2.18 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
2.3.2.19 ribostamycin:4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoate transferase
2.3.2.20 cyclo(L-leucyl-L-phenylalanyl) synthase
2.3.2.21 cyclo(L-tyrosyl-L-tyrosyl) synthase
2.3.2.22 cyclo(L-leucyl-L-leucyl) synthase
2.3.2.23 E2 ubiquitin-conjugating enzyme
2.3.2.24 (E3-independent) E2 ubiquitin-conjugating enzyme
2.3.2.25 N-terminal E2 ubiquitin-conjugating enzyme
2.3.2.26 HECT-type E3 ubiquitin transferase
2.3.2.27 RING-type E3 ubiquitin transferase
2.3.2.28 L-allo-isoleucyltransferase
2.3.2.29 aspartate/glutamate leucyltransferase
2.3.2.30 L-ornithine Nα-acyltransferase
2.3.2.31 RBR-type E3 ubiquitin transferase
2.3.2.32 cullin-RING-type E3 NEDD8 transferase
2.3.2.33 RCR-type E3 ubiquitin transferase
2.3.2.34 E2 NEDD8-conjugating enzyme
2.3.2.35 capsaicin synthase
2.3.2.36 RING-type E3 ubiquitin transferase (cysteine targeting)
2.3.3 Acyl groups converted into alkyl groups on transfer
2.4 Glycosyltransferases
2.5 Transferring alkyl or aryl groups, other than methyl groups
2.6 Transferring nitrogenous groups
2.7 Transferring phosphorus-containing groups
2.8 Transferring sulfur-containing groups
2.9 Transferring selenium-containing groups
2.10 Transferring molybdenum- or tungsten-containing groups
3 Hydrolases
4 Lyases
5 Isomerases
6 Ligases
7 Translocases