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EC 1.14.15.15 Details
EC number
1.14.15.15
Accepted name
cholestanetriol 26-monooxygenase
Reaction
5β-cholestane-3α,7α,12α-triol + 6 reduced adrenodoxin + 6 H+ + 3 O2 = (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oate + 6 oxidized adrenodoxin + 4 H2O (overall reaction);;(1a) 5β-cholestane-3α,7α,12α-triol + 2 reduced adrenodoxin + 2 H+ + O2 = (25R)-5β-cholestane-3α,7α,12α,26-tetraol + 2 oxidized adrenodoxin + H2O;;(1b) (25R)-5β-cholestane-3α,7α,12α,26-tetraol + 2 reduced adrenodoxin + 2 H+ + O2 = (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-al + 2 oxidized adrenodoxin + 2 H2O;;(1c) (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-al + 2 reduced adrenodoxin + 2 H+ + O2 = (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oate + 2 oxidized adrenodoxin + H2O
Other name(s)
5β-cholestane-3α,7α,12α-triol 26-hydroxylase, 5β-cholestane-3α,7α,12α-triol hydroxylase, cholestanetriol 26-hydroxylase, sterol 27-hydroxylase, sterol 26-hydroxylase, cholesterol 27-hydroxylase, CYP27A, CYP27A1, cytochrome P450 27A1′
Systematic name
5β-cholestane-3α,7α,12α-triol,adrenodoxin:oxygen oxidoreductase (26-hydroxylating)
CAS registry number
52227-77-7
Comment
This mitochondrial cytochrome P-450 enzyme requires adrenodoxin. It catalyses the first three sterol side chain oxidations in bile acid biosynthesis via the neutral (classic) pathway. Can also act on cholesterol, cholest-5-ene-3β,7α-diol, 7α-hydroxycholest-4-en-3-one, and 5β-cholestane-3α,7α-diol. The enzyme can also hydroxylate cholesterol at positions 24 and 25. The initial source of the electrons is NADPH, which transfers the electrons to the adrenodoxin via EC 1.18.1.6, adrenodoxin-NADP+ reductase.
History
created 1976 as EC 1.14.13.15, modified 2005, modified 2012, transferred 2016 to EC 1.14.15.15
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