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EC 1.14.99.69 Details
EC number
1.14.99.69
Accepted name
tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase
Reaction
2-(methylsulfanyl)-N6-prenyladenosine37 in tRNA + reduced acceptor + O2 = N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O
Other name(s)
miaE (gene name), tRNA 2-methylthio-N6-isopentenyl adenosine(37) hydroxylase, tRNA 2-(methylsulfanyl)-N6-dimethylallyladenosine37 hydroxylase
Systematic name
tRNA 2-(methylsulfanyl)-N6-prenyladenosine37,donor:oxygen 4-oxidoreductase (trans-hydroxylating)
Comment
The enzyme, found only within a small subset of facultative anaerobic bacteria, belongs to the nonheme diiron family. The enzyme from Salmonella typhimurium was shown to catalyse a stereoselective (E)-hydroxylation at the terminal C4-position of the prenyl group.
History
created 2020
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