EC Explorer

1.14.15.38

N,N-dimethyl phenylurea N-demethylase

an N,N-dimethyl-N′-phenylurea compound + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H⁺ + O₂ = an N-methyl-N′-phenylurea compound + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H₂O

pdmAB (gene names)

N,N-dimethyl-N′-phenylurea compound,NAD(P)H:oxygen oxidoreductase (formaldehyde-forming)

The enzyme, found in members of the Sphingobium genus, initiates the degradation of N,N-dimethyl-phenylurea herbicides by mono-N-demethylation. The catalytic unit contains a Rieske [2Fe-2S] iron-sulfur cluster, and catalyses the monooxygenation of a methyl group. The resulting N-methoxyl group is unstable and decomposes spontaneously to form formaldehyde. The enzyme associates with additional proteins (a reductase and a [3Fe-4S] type ferredoxin) that are involved in the transfer of electrons from NAD(P)H to the active site.

created 2020