Any feedback?
Please rate this page
(ecexplorer.php)
(0/150)

BRENDA support

EC Explorer

EC 1.17.3.2 Details
EC number
1.17.3.2
Accepted name
xanthine oxidase
Reaction
xanthine + H2O + O2 = urate + H2O2
Other name(s)
hypoxanthine oxidase, hypoxanthine:oxygen oxidoreductase, Schardinger enzyme, xanthine oxidoreductase, hypoxanthine-xanthine oxidase, xanthine:O2 oxidoreductase, xanthine:xanthine oxidase
Systematic name
xanthine:oxygen oxidoreductase
CAS registry number
9002-17-9
Comment
An iron-molybdenum flavoprotein (FAD) containing [2Fe-2S] centres. Also oxidizes hypoxanthine, some other purines and pterins, and aldehydes, but is distinct from EC 1.2.3.1, aldehyde oxidase. Under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 O2.- + 2 H+. The mammalian enzyme predominantly exists as an NAD-dependent dehydrogenase (EC 1.17.1.4, xanthine dehydrogenase). During purification the enzyme is largely converted to the O2-dependent xanthine oxidase form (EC 1.17.3.2). The conversion can be triggered by several mechanisms, including the oxidation of cysteine thiols to form disulfide bonds [4,5,7,10] [which can be catalysed by EC 1.8.4.7, enzyme-thiol transhydrogenase (glutathione-disulfide) in the presence of glutathione disulfide] or limited proteolysis, which results in irreversible conversion. The conversion can also occur in vivo [4,6,10].
History
created 1961 as EC 1.2.3.2, transferred 1984 to EC 1.1.3.22, modified 1989, transferred 2004 to EC 1.17.3.2, modified 2011
EC Tree