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1.5.99.15: dihydromethanopterin reductase (acceptor)

This is an abbreviated version!
For detailed information about dihydromethanopterin reductase (acceptor), go to the full flat file.

Reaction

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5,6,7,8-tetrahydromethanopterin
+
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oxidized acceptor
=
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7,8-dihydromethanopterin
+
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reduced acceptor

Synonyms

AfpA, archaeal-flavoprotein-like flavoprotein, Bxe_B2440, dihydromethanopterin reductase, Dmr, DmrB, DmrX, H2MPT reductase, methylene H4MPT dehydrogenase B, MJ0208, MM1854, MtdB

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.99 With unknown physiological acceptors
                EC 1.5.99.151.5.99.15 dihydromethanopterin reductase (acceptor)

Purification

Purification on EC 1.5.99.15 - dihydromethanopterin reductase (acceptor)

for references in articles please use BRENDA:EC1.5.99.15

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and S200 gel filtration
recombinant His6-tagged enzyme from Escherichia col strain BE100 [BL21(DE3) RIL] in an anaerobic chamber by heat treatement at 65┬░C for 5 min, the supernatant is a second time heat treated at 85┬░C for 15 min,
recombinant His6-tagged enzyme from Escherichia coli strain BE100 [BL21(DE3) RIL] in an anaerobic chamber by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant N-terminally His6 -tagged enzyme from Escherichia coli strain BL21DE3 RIL cell-free extract by nickel affinity chromatography and gel filtration