1.5.99.15: dihydromethanopterin reductase (acceptor)
This is an abbreviated version!
For detailed information about dihydromethanopterin reductase (acceptor), go to the full flat file.
Reaction
Synonyms
AfpA, archaeal-flavoprotein-like flavoprotein, Bxe_B2440, dihydromethanopterin reductase, Dmr, DmrB, DmrX, H2MPT reductase, methylene H4MPT dehydrogenase B, MJ0208, MM1854, MtdB
ECTree
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Crystallization
Crystallization on EC 1.5.99.15 - dihydromethanopterin reductase (acceptor)
for references in articles please use BRENDA:EC1.5.99.15
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hanging drop vapor diffusion method, using 0.8-1.2 M ammonium sulfate as precipitant
purified recombinant N-terminally His6 -tagged enzyme by hanging drop vapor diffusion method, mixing of 0.003 ml of 13 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5% glycerol, and 4 mM DTT, with reservori solution containing FMN, method optimization, 7-14 days at room temperature, X-ray diffraction structure determination and analysis at 1.9 A resolution