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1.5.99.15: dihydromethanopterin reductase (acceptor)

This is an abbreviated version!
For detailed information about dihydromethanopterin reductase (acceptor), go to the full flat file.

Reaction

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5,6,7,8-tetrahydromethanopterin
+
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oxidized acceptor
=
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7,8-dihydromethanopterin
 +
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reduced acceptor

Synonyms

AfpA, archaeal-flavoprotein-like flavoprotein, Bxe_B2440, dihydromethanopterin reductase, Dmr, DmrB, DmrX, H2MPT reductase, methylene H4MPT dehydrogenase B, MJ0208, MM1854, MtdB

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.99 With unknown physiological acceptors
                EC 1.5.99.151.5.99.15 dihydromethanopterin reductase (acceptor)

Cloned

Cloned on EC 1.5.99.15 - dihydromethanopterin reductase (acceptor)

for references in articles please use BRENDA:EC1.5.99.15

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3)-RIL cells
expression in Escherichia coli
gene dmrB, recombinant expression of N-terminally His6 -tagged enzyme in Escherichia coli strain BL21DE3 RIL
gene dmrX, recombinant expression of His6-tagged enzyme in Escherichia coli strain BE100 [BL21(DE3) RIL]
gene dmrX, recombinant expression of the codon-optimized gene encoding His6-tagged enzyme in Escherichia col strain BE100 [BL21(DE3) RIL]