1.3.99.30: phytoene desaturase (3,4-didehydrolycopene-forming)
This is an abbreviated version!
For detailed information about phytoene desaturase (3,4-didehydrolycopene-forming), go to the full flat file.
Reaction
Synonyms
Al-1, CarB, CrtI, PDS, phytoene desaturase
ECTree
1.3.99.30 phytoene desaturase (3,4-didehydrolycopene-forming)Advanced search results
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results (| Results | in table |
|---|---|
| 2 | Activating Compound |
| 12 | AA Sequence |
| 6 | Cloned(Commentary) |
| 1 | Cofactor |
| 23 | General Information |
| 2 | KM Value [mM] |
| 7 | Localization |
| 1 | Molecular Weight [Da] |
| 38 | Natural Substrates/ Products (Substrates) |
| 14 | Organism |
| 3 | Pathway |
| 1 | pH Optimum |
| 1 | pI Value |
| 7 | Protein Variants |
| 7 | Reaction |
| 13 | Reference |
| 57 | Substrates and Products (Substrate) |
| 3 | Subunits |
| 33 | Synonyms |
| 1 | Systematic Name |
| 1 | Temperature Optimum [°C] |
Cloned
Cloned on EC 1.3.99.30 - phytoene desaturase (3,4-didehydrolycopene-forming)
for references in articles please use BRENDA:EC1.3.99.30
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli. Generation of a 3,4-didehydrolycopene accumulating Escherichia coli strain
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gene al-1, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli
gene carB, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli
overexpression in Xanthophyllomyces dendrorhous. To achieve this, the plasmids pPR40F and pPR40R are introduced in Xanthophyllomyces dendrorhous CBS 6938. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
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