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1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
+
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

Storage Stability

Storage Stability on EC 1.1.3.6 - cholesterol oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-5°C, crystallized enzyme in lyophilized state, in the dark, stable for at least 10 months
-
30°C, purified enzyme, completely stable for 60 days, 24% reduction after 70 days, 60% reduction after 100 days
-
4-8°C, purified native enzyme, the enzyme maintains approximately 36% and 58.5% of its activity after 18 days of storage
-
4°C, ChOx-immobilized silk mat, when the measurements are made at 3 days interval the immobilized ChOx can retain about 50% of its original activity after 45 days of storage and when the same carried out with 7 days interval, about 70% of its original activity is retained till 60 days
-
4°C, enzyme immobilized on polyacrylonitrile hollow fibers retains 80% of its activity after 15 days, native enzym retains about 55% of its activity after 15 days
-
4°C, purified enzyme, completely stable for 60 days, 24% reduction after 70 days, 60% reduction after 100 days
-
95°C, purified natie enzyme, the enzyme loses activity by gradual thermal treatment, but it maintains 58.5% activity at 95°C after 2 h
-