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1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
+
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

Specific Activity

Specific Activity on EC 1.1.3.6 - cholesterol oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.045 - 0.069
Nocardia rhodochrous
-
substrate cholesterol
0.049
-
purified recombinant mutant V191A, oxidase activity, pH 7.0, 22°C
0.061
-
purified recombinant mutant M122A, oxidase activity, pH 7.0, 22°C
0.074
-
purified recombinant mutant F359A, oxidase activity, pH 7.0, 22°C
0.1
purified full-length isozyme ChoM2, substrate pregnenolone, pH 7.5, 37°C
0.12
purified truncated isozyme ChoM2, substrate pregnenolone, pH 7.5, 37°C
0.13
purified isozyme ChoM1, substrate pregnenolone, pH 7.5, 37°C
0.15
-
purified recombinant mutant V124A, oxidase activity, pH 7.0, 22°C
0.16
purified isozyme ChoM1, substrate dehydroandrosterone, pH 7.5, 37°C
0.17
purified truncated isozyme ChoM2, substrate dehydroandrosterone, pH 7.5, 37°C
0.21
purified full-length isozyme ChoM2, substrate diosgenin, pH 7.5, 37°C
0.23
purified isozyme ChoM1, substrate cholesterol, pH 7.5, 37°C
0.24
native crude enzyme intracellular in strain JC-12, both ChoM isozymes, pH 7.5, 40°C
0.25
purified full-length isozyme ChoM2, substrate beta-sitosterol, pH 7.5, 37°C
0.26
purified truncated isozyme ChoM2, substrate diosgenin, pH 7.5, 37°C
0.33
purified truncated isozyme ChoM2, substrate beta-sitosterol, pH 7.5, 37°C
0.42
purified truncated isozyme ChoM2, substrate cholesterol, pH 7.5, 37°C
0.6
-
polyethylene glycol derivative-modified enzyme
0.78
native crude enzyme extracellular in strain JC-12, both ChoM isozymes, pH 7.5, 40°C
1.39
-
purified enzyme, pH 7.5, 37°C
11.2
purified recombinant His-tagged isozyme ChoM1, pH 7.5, 40°C
12
-
N-terminally His-tagged recombinant enzyme expressed in Escherichia coli, pH 7.2, 37°C
124.87
-
purified enzyme, pH 7.5, 37°C
15
-
pH 8.0, 40°C
15.2
-
strain ST-200
15.5
purified His-tagged enzyme, pH 6.75, 35°C
16
-
non-tagged recombinant enzyme expressed in Escherichia coli, pH 7.2, 37°C
16.08
-
pH 7.0, 37°C
17.2
purified recombinant His-tagged isozyme ChoM2, pH 7.5, 40°C
17.5
-
native enzyme from Brevibacterium sp., pH 7.2, 37°C
2.42
-
inducible enzyme
2.7
-
purified recombinant wild-type enzyme, oxidase activity, pH 7.0, 22°C
20
-
purified enzyme, pH 7.2, 50°C
20.81
-
purified enzyme, pH 7.0, 37°C
21.8
-
purified enzyme, pH 7.0, 37°C
26.7
-
S103T/V145A/V121A/R135H quadruple mutant
3.65
-
constitutive enzyme
35.64
-
purified enzyme, pH 8.0, 37°C
39.9
-
S103T/V145A double mutant
4
-
C-terminally His-tagged recombinant enzyme expressed in Escherichia coli, pH 7.2, 37°C
47.8
-
S103T/V145A/V121A triple mutant
55.2
-
cholesterol oxidase II
63.5
-
V145E mutant, 37°C, pH 7.0
67.7
-
wild type
68.9
-
R135H mutant, 37°C, pH 7.0
72
-
V121A mutant, 37°C, pH 7.0
86.6
-
S103T mutant, 37°C, pH 7.0
additional information