1.1.3.6: cholesterol oxidase
This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.
Word Map on EC 1.1.3.6
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1.1.3.6
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biosensors
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electrode
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esterase
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electrochemical
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brevibacterium
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fabric
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oxidases
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amperometric
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film
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rhodococcus
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raft
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cholestenone
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voltammetry
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cholest-4-en-3-one
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flavoenzyme
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cholesteryl
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nanocomposite
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biosensing
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erythropolis
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filipin
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4-cholesten-3-one
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nocardia
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medicine
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3hcholesterol
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electropolymerization
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lavendulae
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biotechnology
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agriculture
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co-immobilized
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methyl-beta-cyclodextrin
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diagnostics
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bioelectrode
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luminol
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screen-printed
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electrocatalytic
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analysis
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3.1.1.13
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polypyrrole
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multiwalled
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beta-ol
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synthesis
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drug development
- 1.1.3.6
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biosensors
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electrode
- esterase
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electrochemical
- brevibacterium
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fabric
- oxidases
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amperometric
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film
- rhodococcus
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raft
- cholestenone
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voltammetry
- cholest-4-en-3-one
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flavoenzyme
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cholesteryl
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nanocomposite
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biosensing
- erythropolis
- filipin
- 4-cholesten-3-one
- nocardia
- medicine
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3hcholesterol
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electropolymerization
- lavendulae
- biotechnology
- agriculture
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co-immobilized
- methyl-beta-cyclodextrin
- diagnostics
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bioelectrode
- luminol
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screen-printed
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electrocatalytic
- analysis
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3.1.1.13
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polypyrrole
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multiwalled
- beta-ol
- synthesis
- drug development
Reaction
Synonyms
3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx
ECTree
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Application
Application on EC 1.1.3.6 - cholesterol oxidase
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agriculture
analysis
biotechnology
diagnostics
drug development
medicine
synthesis
the enzyme is used for the production of steroid drugs and insecticides
agriculture
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recombinant expression in plants gives insect resistance
agriculture
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recombinant expression in plants gives insect resistance
agriculture
Streptoverticillum cholesterolicum
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recombinant expression in plants gives insect resistance
agriculture
recombinant expression in plants gives insect resistance
agriculture
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strain A19249, enzyme exhibits a potent insecticidal activity
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agriculture
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recombinant expression in plants gives insect resistance
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platinum electrodes modified with thiolipid/lipid bilayer membranes. Cholesterol oxidase spontaneously inserts into the electrode-supported lipid bilayer membrane from solution and is consequently immobilized to the electrode surface. This electrode architecture may be useful in clinical sensing applications
analysis
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the cholesterol oxidase-immobilized polyacrylonitrile hollow fiber may have an industrial or medical application for cholesterol determination or oxidation
analysis
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the enzyme can be used as cholesterol biosensor co-immobilized with cholesterol esterase on oxygen electrodes, determination of total cholesterol in food samples
analysis
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Cellulomonas enzyme is analytically reliable when used for serum cholesterol determination by the endpoint method. Its analytical performance is equivalent to Streptomyces enzymes and meets the analytical goals. It has an advantage over the other enzymes in that it does not ship in the frozen state
analysis
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cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) can be immobilized onto tetraethylorthosilicate derived sol-gel films. These tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films can be used for the estimation of cholesterol oleate up to 780 mg/dl (mM). These TEOS sol-gel enzyme films provide selectivity towards glucose, lactate, uric acid and ascorbic acid
analysis
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simple, reliable, fast and reproducible method to prepare cholesterol oxidase electrodes is described
analysis
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the steady state amperometric measurements of free cholesterol are performed by an enzyme electrode which is developed through electrostatic immobilization of the cholesterol oxidase in poly(vinylferrocenium) perchlorate, PVF+ClO4-, film that is coated on a Pt electrode
analysis
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the development of an enzyme-based sensor employing the enzyme ChOx has great potential as a simple and economical sensor system, engineering of the enzyme for electrochemical monitoring of cholesterol, overview
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Nocardia rhodochrous
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Nocardia erythropolis
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Pseudomonas spp.
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Streptoverticillum cholesterolicum
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
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Cholesterol oxidase has two major biotechnological applications, i.e. in the determination of serum (and food) cholesterol levels and as biocatalyst providing valuable intermediates for industrial steroid drug production
biotechnology
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
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the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium
diagnostics
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the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium
diagnostics
Nocardia rhodochrous
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analytic tool for determining cholesterol in various samples
diagnostics
Nocardia erythropolis
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analytic tool for determining cholesterol in various samples
diagnostics
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analytic tool for determining cholesterol in various samples
diagnostics
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analytic tool for determining cholesterol in various samples
diagnostics
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analytic tool for determining cholesterol in various samples
diagnostics
Streptoverticillum cholesterolicum
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analytic tool for determining cholesterol in various samples
diagnostics
analytic tool for determining cholesterol in various samples
diagnostics
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the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase
diagnostics
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determination of cholesterol, by rapid, cheap and reliable methods, is very important in clinical diagnosis because its level in blood is closely related to human health. A reproducible cholesterol biosensors is prepared based on the direct adsorption of ChOx onto gold substrates (ChOx-Au)
diagnostics
the enzyme is an important biotechnological tool for clinical diagnostics. It is also used for tracking intracellular cholesterol. Its utility is limited by the lack of an efficient temporal control of its activity. A rapamycin-inducible fluorescent cholesterol oxidase from Chromobacterium sp. DS-1 (split GFP-COase) is engineered. Induction by rapamycin allows temporal control over enzyme activity and rapidly leads to an efficient reduction of intracellular cholesterol. It is proposed that this drug-inducible split GFP-COase is a new tool to manipulate the cellular cholesterol content and gain insights into cholesterol-dependent proteins and cholesterol-related cell pathologies
diagnostics
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analytic tool for determining cholesterol in various samples
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diagnostics
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the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase
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the cytotoxicity activity against a few cancer cell lines, while broadly sparing the normal cells, suggests the possible use of this enzyme as a promising future contender for anticancer therapy
drug development
Streptomyces sp. AKHSS
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the enzyme showed significant cytotoxicity on breast (MCF-7), nasopharyngeal (KB) and ovarian (OVCAR) cancer cell lines at very low concentrations ranging from 0.000093 to 0.00014 mM. It exhibits relatively less cytotoxicity on primary mouse embryonic fibroblast (3T3) cells. Thus, cholesterol oxidase from Streptomyces sp. AKHSS could be a potential anticancer agent
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
Nocardia rhodochrous
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
Nocardia erythropolis
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
Corynebacterium cholesterolicum
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
Rhodococcus sp. GK1
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
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