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1.1.3.10: pyranose oxidase

This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.

Word Map on EC 1.1.3.10

Reaction

D-glucose
+
O2
=
2-dehydro-D-glucose
 +
H2O2

Synonyms

C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.10 pyranose oxidase

Specific Activity

Specific Activity on EC 1.1.3.10 - pyranose oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.011
Trametes cinnabarinus
-
-
0.12
Polyporus obtusus
-
-
0.27
-
V546C/T169G/L537W mutant, substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.31
-
unpurified enzyme, at pH 6.5 and 25°C
0.34
-
V546C/T169G/L537W mutant, substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.38
-
V546C/T169G mutant, substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.43
-
V546C/T169G mutant, substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.58
-
purified recombinant His-tagged enzyme, pH 6.5, 30°C
1.16
-
purified recombinant His-tagged enzyme, pH 6.5, 30°C
10.2
mutant K312E, pH 6.5, 30°C
103
-
V546C/T169G/L537W mutant, substrate 1,4-benzoquinone (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
12.8
Polyporus obtusus
-
D-glucose as substrate
13.4
mutant enzyme E540K with a C-terminal His6-tag
189
-
V546C/T169G mutant, substrate 1,4-benzoquinone (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
2.4
mutant T166A/E539K, pH 6.5, 30°C
2.74
recombinant wild-type enzyme
221
-
V546C/T169G mutant, substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
24.1
-
V546C/T169G/L537W mutant, substrate 1,4-benzoquinone (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
246
-
V546C/E542K mutant, substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
261
-
V546C/T169G mutant, substrate ferricenium hexafluorophosphate (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
33.3
-
purified enzyme
4.4
Polyporus obtusus
-
D-xylose as substrate
42.1
-
V546C/T169G mutant, substrate 1,4-benzoquinone (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
5.3
mutant E359K, pH 6.5, 30°C
5.54
-
V546C/E542K mutant, substrate ferricenium hexafluorophosphate (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
51.9
-
V546C/T169G/L537W mutant, substrate ferricenium hexafluorophosphate (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
56.4
-
V546C/T169G/L537W mutant, substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
6.13
-
V546C/E542K mutant, substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
6.2
mutant K312E/E539K, pH 6.5, 30°C
6.4
wild-type, pH 6.5, 30°C
664
-
V546C/E542K mutant, substrate 1,4-benzoquinone (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2' azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
8.15
-
purified enzyme
8.5
Polyporus obtusus
-
purified enzyme, at pH 5.0 and 25°C
8.6
Polyporus obtusus
-
D-gluconolactone as substrate
8.64
-
after 27.9fold purification, at pH 6.5 and 25°C
9.4
Polyporus obtusus
-
L-sorbose as substrate
additional information