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1.1.3.10: pyranose oxidase

This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.

Word Map on EC 1.1.3.10

Reaction

D-glucose
+
O2
=
2-dehydro-D-glucose
+
H2O2

Synonyms

C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.10 pyranose oxidase

KM Value

KM Value on EC 1.1.3.10 - pyranose oxidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0052 - 2
1,4-benzoquinone
10.7
1,5-anhydro-D-glucitol
-
pH 7.0, 37°C
0.07 - 0.09
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical
0.051 - 0.39
2,6-dichloroindophenol
0.065 - 0.094
2,6-dichlorophenolindophenol
0.4 - 2.1
2,6-dimethyl-1,4-benzoquinone
0.31 - 0.48
2-chloro-1,4-benzoquinone
10.32
2-deoxy-D-galactose
100 mM ethanolamine buffer, pH 10.5
11.3 - 99.3
2-deoxy-D-glucose
0.21 - 0.42
2-methoxy-1,4-benzoquinone
0.11 - 0.51
2-methyl-1,4-benzoquinone
3.9
5-thioglucose
-
-
120
6-O-alpha-D-galactopyranosyl-beta-D-glucopyranose
-
-
1 - 1.53
alpha-D-glucose
0.57 - 0.97
beta-D-glucose
290
cellobiose
-
at pH 6.5 and 25°C
22.1 - 68
D-allose
174
D-arabinose
-
pH 7.0, 37°C
0.71
D-cellobiose
100 mM ethanolamine buffer, pH 10.5
103 - 2558
D-fructose
6.25
D-fucose
100 mM ethanolamine buffer, pH 10.5
0.093 - 1260
D-galactose
51.1
D-glucono-1,5-lactone
-
at pH 6.5 and 25°C
38
D-gluconolactone
-
-
0.018 - 240
D-glucose
6.02
D-maltoheptaose
100 mM ethanolamine buffer, pH 10.5
6.14
D-maltopentaose
100 mM ethanolamine buffer, pH 10.5
329
D-maltose
-
pH 7.0, 37°C
6.61
D-maltotriose
100 mM ethanolamine buffer, pH 10.5
110
D-mannoheptose
-
-
295 - 370
D-mannose
2 - 1500
D-melibiose
180
D-ribose
-
with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C
7.51
D-trehalose
100 mM ethanolamine buffer, pH 10.5
2.4 - 78.4
D-xylose
0.092 - 1.12
ferricenium hexafluorophosphate
0.054 - 0.408
ferricenium ion
3.43
ferricyanide
substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.015 - 0.4
ferrocenium hexafluorophosphate
0.187
ferrocenium ion
using D-glucose as cosubstrate, at 30°C, pH 6.5
62
gentibiose
-
-
0.79 - 0.85
glucose
1.55 - 97
L-arabinose
2.9 - 176
L-sorbose
46.4 - 257
maltose
72.8
melibiose
at 30°C, pH 6.5
0.35 - 0.4
methyl-1,4-benzoquinone
50 - 289
methyl-beta-D-glucoside
0.03 - 4.8
O2
0.0832 - 0.65
oxygen
902
sucrose
-
at pH 6.5 and 25°C
0.09
tetrabromo-1,4-benzoquinone
0.088
tetrachloro-1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.22 - 9.42
tetrafluoro-1,4-benzoquinone
192
trehalose
-
pH 7.0, 37°C
additional information
additional information
-