1.1.1.B51: 3-quinuclidinone reductase (NADPH)

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For detailed information about 3-quinuclidinone reductase (NADPH), go to the full flat file.

Reaction

Synonyms

RrQR

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.B51 3-quinuclidinone reductase (NADPH)

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Results	in table
1	AA Sequence
5	Application
3	Cloned(Commentary)
3	Cofactor
2	Crystallization (Commentary)
3	Inhibitors
7	KM Value [mM]
2	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
5	Organism
4	pH Optimum
11	Protein Variants
2	Purification (Commentary)
1	Reaction
5	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
11	Substrates and Products (Substrate)
4	Subunits
1	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
5	Turnover Number [1/s]

Cloned

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Cloned on EC 1.1.1.B51 - 3-quinuclidinone reductase (NADPH)

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an expression vector, pWKLQ, which contains the full length 3-quinuclidinone reductase gene is constructed. Using Escherichia coli cells coexpressing the 3-quinuclidinone reductase and glucose dehydrogenase (cofactor regeneration enzyme) genes, 618 mM 3-quinuclidinone is almost stiochiometrically converted to (R)-3-quinuclidinol with an >99.9% enantiomeric excess within 21 h of reaction

Rhodotorula mucilaginosa

B9ZZZ6

729053

expressed in Escherichia coli

Rhodotorula mucilaginosa

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