1.1.1.67: mannitol 2-dehydrogenase
This is an abbreviated version!
For detailed information about mannitol 2-dehydrogenase, go to the full flat file.
Word Map on EC 1.1.1.67
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1.1.1.67
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polyol
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fluorescens
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d-fructose
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synthesis
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5-dehydrogenase
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1.1.1.138
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heterofermentative
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reuteri
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gluconobacter
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industry
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pfldh
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arabitol
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molasses
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nutrition
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biotechnology
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analysis
- 1.1.1.67
-
polyol
- fluorescens
- d-fructose
- synthesis
-
5-dehydrogenase
-
1.1.1.138
-
heterofermentative
- reuteri
- gluconobacter
- industry
- pfldh
- arabitol
- molasses
- nutrition
- biotechnology
- analysis
Reaction
Synonyms
alcohol dehydrogenase, zinc-containing, D-mannitol dehydrogenase, M2DH, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NAD+-dependent mannitol dehydrogenase, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, TM_0298
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Specific Activity
Specific Activity on EC 1.1.1.67 - mannitol 2-dehydrogenase
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33
after 6.7fold purification, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1
54
63
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MDH expressed in Escherichia coli strain BL21 (DE3) plysS, with fructose as substrate
additional information
purified enzyme at 80°C with D-fructose as the substrate and NADH as the cofactor
54
purified enzyme, at 80°C with fructose as the substrate and NADH as the cofactor
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the activity for inositol, sorbitol, galactitol, ribitol, xylitol, arabitol and erythritol is very low
additional information
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significant yield of D-mannitol (approximately 40%) from 1% w/v D-fructose by Thermotoga neapolitana MtDH