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1.1.1.67: mannitol 2-dehydrogenase

This is an abbreviated version!
For detailed information about mannitol 2-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.67

Reaction

D-mannitol
+
NAD+
=
D-fructose
 +
NADH
 +
H+

Synonyms

alcohol dehydrogenase, zinc-containing, D-mannitol dehydrogenase, M2DH, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NAD+-dependent mannitol dehydrogenase, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, TM_0298

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.67 mannitol 2-dehydrogenase

Cloned

Cloned on EC 1.1.1.67 - mannitol 2-dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BLR(DE3) cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli strain BL21pLysS
-
expression in Escherichia coli
expression in Escherichia coli, effecting strong catalytic activity of an NADH-dependent reduction of D-fructose to D-mannitol in cell extracts of the recombinant Escherichia coli strain
expression of wild-type and mutant enzymes in Escherichia coli strain JM109
expression of wild-type enzyme and mutant enzymes in Escherichia coli
-
fusion of six His codons to the 3'-end of the mdh gene and expression in Escherichia coli M15. The enzyme shares significant sequence similarity with the medium-chain dehydrogenase/reductase protein family
gene mdh, high level expression in Bacillus megaterium requiring the adaptation of the corresponding ribosome binding site, the fdh gene is adapted to Bacillus megaterium codon usage via complete chemical gene synthesis, overview
gene mtdh, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene mtlD, recombinant overexpression of the enzyme in Escherichia coli strain JM109
MtDH is expressed in Escherichia coli BL21(DE3)
subcloned into vector pDEST110 and overexpressed in different strains of Escherichia coli (BL21 (DE3) plysS, JM109, Origami(DE3) or M15)
-
the TM0298 gene subcloned into the NdeI and XhoI sites of pET24a(+) to yield plasmid pTmMtDH, from which MtDH is expressed with a C-terminal His6-tag in Escherichia coli BL21(DE3)