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1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating)

This is an abbreviated version!
For detailed information about malate dehydrogenase (oxaloacetate-decarboxylating), go to the full flat file.

Word Map on EC 1.1.1.38

Reaction

(S)-malate
+
NAD+
=
pyruvate
+
CO2
+
NADH
+
H+

Synonyms

m-NAD(P)-ME, maeA, malic enzyme, malic enzyme 2, ME2, MHD, mitochondrial malic enzyme ME2, mitochondrial NAD(P) malic enzyme, mitochondrial NAD(P) malic enzyme ME2, mitochondrial NAD(P)+-dependent malic enzyme, mitochondrial NAD-malic enzyme, NAD+-dependent malic enzyme, NAD-dependent ME, NAD-malic enzyme, NAD-ME, NAD-specific malic enzyme, NADH-dependent malic enzyme, pyruvic-malic carboxylase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.38 malate dehydrogenase (oxaloacetate-decarboxylating)

Purification

Purification on EC 1.1.1.38 - malate dehydrogenase (oxaloacetate-decarboxylating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
38-40°c treatment, ammonium sulfate, protamine sulfate precipitation, calcium phosphate gel, ammonium sulfate
-
ammonium sulfate, DEAE-cellulose, Blue-B agarose, Orange-A agarose
-
DEAE Sepharose CL-6B column chromatography, and Q Sepharose column chromatography
-
MnCl2 precipitation, protaminsulfate precipitation, Sephadex G-25, hydroxylapatite
-
MnCl2 precipitation, protaminsulfate precipitation, Sephadex G-25, Sephadex G-200, hydroxylapatite
-
protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-cellulose
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and ultrafiltration to 95% purity
-
streptomycin sulfate ammonium sulfate, Phenyl-sepharose, Orange-A-Sepharose
-
streptomycin, ammonium sulfate, heat treatment, acid treatment, calcium phosphate gel, DEAE-sephadex, hydroxylapatite, Sepharose 6B
-