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1.1.1.315: 11-cis-retinol dehydrogenase

This is an abbreviated version!
For detailed information about 11-cis-retinol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.315

Reaction

11-cis-retinol-[retinal-binding-protein]
+
NAD+
=
11-cis-retinal-[retinol-binding-protein]
+
NADH
+
H+

Synonyms

11-cis RD, 11-cis-RDH, 11-cis-retinol dehydrogenase, 11-cis-Ro-DH, atRDH, cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase, CRAD, CRAD2, cRDH, EC 5.2.1.3, RDH10, RDH5, retinol dehydrogenase 10

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.315 11-cis-retinol dehydrogenase

Cloned

Cloned on EC 1.1.1.315 - 11-cis-retinol dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in CHO cells
expressed in COS1 cells
expression in CHO cells
expression in CHO-K1 cells
expression in Cos-1 cells
expression in HEK-293 cell
-
expression in Hi-5 insect cells
expression in Sf9 cell
RDH10 is expressed in COS cells
RDH10 is expressed the enzyme in insect Sf9 cells using the Baculovirus expression system. Purification of RDH10-His6 from Sf9 cells using nickel affinity chromatography produces an inactive enzyme. Therefore, microsomal preparations of RDH10 are used for its kinetic characterization