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1.1.1.270: 3beta-hydroxysteroid 3-dehydrogenase

This is an abbreviated version!
For detailed information about 3beta-hydroxysteroid 3-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.270

Reaction

a 3beta-hydroxysteroid
+
NADP+
=
a 3-oxosteroid
 +
NADPH
 +
H+

Synonyms

17beta-HSD7, 17beta-hydroxysteroid dehydrogenase type 7, 3-keto reducing enzyme, 3-keto reductase, 3-keto-reductase, 3-ketoreductase, 3-ketosteroid reductase, 3-KSR, 3-oxo steroid reductase, 3-oxosteroid reductase, 3beta-HSD, 3beta-HSD1, 3beta-HSD2, 3beta-HSDH, 3beta-hydroxysteroid dehydrogenase, 3beta-hydroxysteroid dehydrogenase type 1, 3beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type 1, 3beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type 2, 3beta-hydroxysteroid dehydrogenase/isomerase, AKR1C1, AKR1C2, AKR1Cs, aldo-keto reductase 1C2, CDD59473, Erg27, Erg27p, Hsd17, HSD17B7, HSD3B1, reductase, 3-oxo steroid, reductive 3beta-HSD, SDR11E1, SDR11E2, steroid-3-ketoreductase, sterol 3-ketoreductase, sterone-reducing enzyme, type 3 3alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.270 3beta-hydroxysteroid 3-dehydrogenase

Cloned

Cloned on EC 1.1.1.270 - 3beta-hydroxysteroid 3-dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3-ketosteroid reductase activity of the human 3(alpha-beta)-hydroxysteroid epimerase, stable expression
-
DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) pLysS
-
expression of HSD17B7 and truncated HSD17B7 (shortened by 127 amino acids at the C terminus) as glutathione-S-transferase fusion protein in Escherichia coli. Erg27p-deficient yeast strain complements the 3-ketosteroid reductase deficiency of the cells and restores growth on sterol-deficient medium
-
expression of HSD17B7 as a glutathione-S-transferase fusion protein in Escherichia coli. Erg27p-deficient yeast strain complements the 3-ketosteroid reductase deficiency of the cells and restores growth on sterol-deficient medium
-
expression of wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
-
heterologous expression in Escherichia coli
isoform type III of 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase with almost exclusive 3-ketosteroid reductase activity, cDNA expression
-
overexpression in Escherichia coli
overexpression in Escherichia coli BL21 (DE3) Codon Plus RP cells
quantitative expression analysis of AKR1C isozymes
-
the cDNA that encodes the mutant 3beta-HSD1 enzymes S194G-1 and R195P-1 are generated via double-stranded, PCR-based mutagenesis and inserted into baculovirus. The recombinant baculovirus system successfully expresses the mutant S194G-1 and R195P-1 enzyme proteins in Sf9 cells with the wild-type 3beta-HSD1 as the control