Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.1.21: aldose reductase

This is an abbreviated version!
For detailed information about aldose reductase, go to the full flat file.

Word Map on EC 1.1.1.21

Reaction

alditol
+
NAD(P)+
=
aldose
+
NAD(P)H
+
H+

Synonyms

17betaHSD5, 20-alpha-HSD, 20-alpha-hydroxysteroid dehydrogenase, AdhA, AKR1B, AKR1B1, AKR1B10, AKR1B13, AKR1B14, AKR1B3, AKR1C3, AKR4C7, aldehyde reductase, alditol/NADP+ oxidoreductase, alditol: NADP+ 1-oxidoreductase, alditol:NAD(P)+1oxidoreductase, alditol:NADP 1-oxidoreductase, alditol:NADP oxidoreductase, aldo-keto reductase, aldo-keto reductase family 1 member B1, aldo-keto reductase family 1 member B7, aldoketo reductase 1C3, aldose reductase, aldose reductase 2, aldose reductase-like, aldose reductase-like protein, aldose xylose reductase, ALDRXV4, ALR, ALR1, ALR2, AR, ARI, BAR, EC 1.1.1.139, Fibroblast growth factor regulated protein, FR-1 protein, GRE3, HAR, HRAR, isobutyraldehyde reductase, More, MVDP, NADPH dependent xylose reductase, NADPH-aldopentose reductase, NADPH-aldose reductase, polyol dehydrogenase (NADP2), RLAR, small intestine reductase, TPN-polyol dehydrogenase, VAS deferens androgen-dependent protein, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.21 aldose reductase

Purification

Purification on EC 1.1.1.21 - aldose reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzymes: AR I and AR II
-
AdhA appears to be insensitive to the presence of oxygen
-
agarose-IDA-Ni resin column chromatography, gel filtration
ALR 1 und ALR 2
-
ALR 2
-
ALR2 partially from lenses
-
amylose resin column chromatography
-
Blue Sepharose column chromatography
enzyme from bovine lens
expressed in baculovirus system
-
native enzyme from lens
native enzyme from lens by anion exchange and Matrex Orange A affinity chromatography
-
native enzyme from lens by anion exchange chromatography
-
native enzyme partially from left ventricular tissue, by ultracentrifugation and gel filtration
-
native enzyme partially from lens by ammonium sulfate fractionation and dialysis
native isozymes partially by ammonium sulfate fractionation and dialysis
Ni-NTA column chromatography
-
Ni-NTA resin column chromatography
-
nickel-charged Sepharose resin column chromatography
optimized extraction by cetyl trimethyl ammonium bromide reversed micelles
-
purification from kidney of prehibernating jerboas
-
Q-Sepharose column chromatography and Sephadex G-100 gel filtration
-
Q-Sepharose column chromatography, Sephadex G-100 gel filtration, and Red-Sepharose column chromatography
-
recombinant ALR2 from Escherichia coli, and recombinant AKR1B10 from Escherichia coli strain BL21 by anion exchange and affinity chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS
recombinant His-tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography, removal of the His-tag by thrombin, and anion exchange chromatography to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by thrombin, followed by anion exchange chromatography
recombinant His-tagged enzyme several fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by thrombin, followed by anion exchange chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21 gold (DE3) by nickel affinity chromatography, tag cleavage by thrombin, followed by anion exchange chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
wild type and expressed in Escherichia coli
wild type and recombinant from Escherichia coli
-