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1.1.1.2: alcohol dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about alcohol dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.2

Reaction

a primary alcohol
+
NADP+
=
an aldehyde
 +
NADPH
 +
H+

Synonyms

2° Adh, 3-DG-reducing enzyme, A3L14_07690, AAur_2040, ADH, ADH-1, ADH-2, ADH-I, ADH-II, ADH2, Adh319, ADH4, Adh6, AdhA, AdhB, AdhD, AKR1A1, AKR1A4, alcohol dehydrogenase C, alcohol dehydrogenase D, alcohol dehydrogenase [NADP(+)], Alcohol dehydrogenase [NADP+], aldehyde reductase, aldehyde reductase (NADPH2), aldehyde/ketone reductase, aldo-keto reductase, Aldo-keto reductase family 1 member A1, aldose reductase, ALDR, alipathic aldehyde reductase, ALR, ALR 1, ALR1, BdhA, bovine brain aldehyde reductase, CbADH, D-glucuronate reductase, daunorubicin reductase, DRD, EhADH1, FeADH, Gre2p, hexogenate dehydrogenase, high-Km aldehyde reductase, HvADH2, iron-containing alcohol dehydrogenase, KpADH, L-hexonate dehydrogenase, LB-RADH, LBADH, liver alcohol dehydrogenase, low-Km aldehyde reductase, mevaldate reductase, Mpd1, NADP(H)-dependent alcohol dehydrogenase, NADP+-dependent alcohol dehydrogenase, NADP-alcohol dehydrogenase, NADP-aldehyde reductase, NADP-dependent aldehyde reductase, NADP-linked aryl alcohol dehydrogenase, NADPH-aldehyde reductase, NADPH-cytochrome c reductase, NADPH-dependent ADH, NADPH-dependent aldehyde reductase, NADPH-dependent FALDR, NADPH-dependent fatty aldehyde reductase, NADPH-linked aldehyde reductase, nonspecific succinic semialdehyde reductase, Octopine dehydrogenase, putative iron alcohol dehydrogenase, PyAeADHII, rabbit kidney aldehyde-ketone reductase, RADH, ScADHVI, short-chain ADH, short-chain alcohol dehydrogenase, short-chain dehydrogenase/reductase, TBADH, TbADH1, Teth39_1597, Teth514_0564, TPN-L-hexonate dehydrogenase, TPNH-linked aldehyde reductase, TPNH-specific aldehyde reductase, triphosphopyridine nucleotide-linked aldehyde reductase, TsAdh319, Y63 protein, yeast alcohol dehydrogenase, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.2 alcohol dehydrogenase (NADP+)

Purification

Purification on EC 1.1.1.2 - alcohol dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1° Adh and 2°Adh
-
aldehyde reductase I
aldehyde reductase I and II
-
aldehyde reductase I and II from brain, no recuctase II activity could be detected in pig kidney
-
aldehyde reductase II
-
by anion-exchange chromatography followed by affinity chromatography on Ni-NTA
-
DEAE-cellulose resin column chromatography, Ni-NTA column chromatography, and Superose 6 gel filtration
-
DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration
native enzyme partially from lens and kidney
one step purification-immobilization on metal chelate-epoxy Sepabeads
-
overexpressed Y63 protein partially purified by Ni-NTA column chromatography
-
Q-Sepharose column chromatography
-
recombinant enzyme from Escherichia coli strain Rosetta2 by heat treatment for 30 min at 75°C, the supernatant is further purified by cation exchange and anion exchange chromatography, ultrafiltration, and gel filtration
recombinant enzyme, purification by nickel ion affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL(DE3) by nickel affinity chromatography
-
reductase I
-
to homogeneity
to near homogeneity by amylose affinity chromatography
Marinobacter nauticus VT8
-
wild-type aldehyde reductase and mutant A H112Q
-
wild-type and mutant enzyme
wild-type and recombinant protein
-