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0.33
(+)-Camphorquinone
-
-
15
(2E,4E)-hexa-2,4-dienal
-
pH 7.4, 25°C, with NAD(P)H
15.2
(2R,3R)-2,3-butanediol
cosubstrate: NADP+, pH 7.5, 80°C
246
(2S,3S)-2,3-butanediol
cosubstrate: NADP+, pH 7.5, 80°C
5.4 - 327.6
(2S,5S)-hexanediol
0.0063
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde)
-
eutypine
0.112
(S)-1-phenylethanol
-
-
0.46 - 0.8
1,2-Cyclohexanedione
15.1
1,4-Butanediol
-
pH 9.5, 30°C, recombinant mutant V84I/Y127M
23.38
1,5-pentanediol
pH 7.0. 30°C
0.00273 - 0.99
1-Pentanol
2.9 - 55.5
1-phenylethanol
56.4 - 79
2,5-hexanedione
0.31
2-butanone
cosubstrate: NADPH, pH 7.5, 80°C
0.85 - 6.38
2-hydroxytetrahydrofuran
-
0.049 - 4.8
2-Methoxybenzaldehyde
1.85 - 17.7
2-methylbutanal
0.05
2-phenylacetaldehyde
-
pH 8.0, 80°C
15
2-phenylethanol
-
pH 8.0, 80°C
6.1
3,4-dihydroxyphenylacetaldehyde
-
-
12
3,4-dihydroxyphenylglycolaldehyde
-
-
0.0054
3-Fluorobenzaldehyde
-
-
10.27
3-methyl-1,5-pentanediol
pH 7.0. 30°C
1.61 - 4.6
3-methyl-1-butanol
0.048 - 1.89
3-Methylbutanal
0.008 - 8.61
3-Pyridinecarboxaldehyde
5.5
3-Pyridinemethanol
-
-
1.6
4-Carboxyacetophenone
-
-
0.1
4-Carboxyphenylglyoxal
-
-
21.8
4-hydroxybenzaldehyde
-
pH 6.5, 30°C
24.1
4-methoxybenzaldehyde
-
pH 6.5, 30°C
1.4
4-Nitroacetophenone
-
-
0.0053
4-nitrobenzaldehyde
-
-
0.0045
4-pyridine carboxaldehyde
-
-
0.062 - 1.2
4-Pyridinecarboxaldehyde
27
5-Hydroxyindoleacetaldehyde
-
-
0.11 - 4
5alpha-dihydrotestosterone
0.32
acetoin
cosubstrate: NADPH, pH 7.5, 80°C
0.034 - 10.9
acetophenone
4.81
acrolein
-
pH 7.0, 37°C
0.008
all-trans-retinal
-
-
0.02
all-trans-retinol
-
-
0.099 - 13
androstane-3,17-dione
0.67
butanaldehyde
-
pH 7.0, 37°C
0.083 - 7.69
Butyraldehyde
0.017 - 0.172
cinnamaldehyde
0.08 - 0.7
cinnamyl alcohol
0.177
cis-11-hexadecenal
Marinobacter nauticus VT8
-
-
0.06
coniferylaldehyde
-
-
1.18 - 36.77
cyclohexanone
8.45 - 19.33
Cyclopentanol
543
D-galactose
-
aldehyde reductase II
1.1
D-galacturonate
-
AR1
1.29 - 2.99
D-glucuronic acid
19.6 - 67
D-glucuronolactone
0.43 - 8.2
D-glyceraldehyde
0.08
daunorubicin
-
reductase I
1.3 - 76
DL-glyceraldehyde
0.091
ethyl 4-bromo-3-oxobutanoate
-
-
0.36
ethyl 4-chloro-3-oxobutanoate
-
-
24 - 25
ethyl acetoacetate
2.7 - 26
ethylacetoacetate
1.6
formaldehyde
-
1° Adh
0.07 - 7.59
glyceraldehyde
0.0038
hydroxyindol-3-ylacetaldehyde
-
low-Km reductase
0.0059 - 0.2
indol-3-ylacetaldehyde
0.6
indolacetaldehyde
-
-
0.32 - 3.7
Indole-3-acetaldehyde
4.9 - 11.1
L-glyceraldehyde
0.31
laurylaldehyde
-
AR2
0.007
m-chlorobenzaldehyde
-
-
0.027 - 0.212
m-methoxybenzaldehyde
0.02
m-methylbenzaldehyde
-
-
0.002 - 4.4
m-nitrobenzaldehyde
1.78
malondialdehyde
-
pH 7.0, 37°C
19.3
meso-2,3-butanediol
cosubstrate: NADP+, pH 7.5, 80°C
1.2
methyl 4-bromo-3-oxobutanoate
1.1 - 150
n-Butyraldehyde
0.702
o-fluorobenzaldehyde
-
-
0.125
o-methylbenzaldehyde
-
-
0.376 - 4.6
o-nitrobenzaldehyde
3.88
octan-1-ol
pH 8, 60°C
0.1
octyl 4-chloro-3-oxobutanoate
-
-
0.004 - 4
p-Carboxybenzaldehyde
0.94 - 6.7
p-chlorobenzaldehyde
0.005 - 0.51
p-cyanobenzaldehyde
0.04
p-fluorobenzaldehyde
-
-
0.0023 - 0.91
p-hydroxyphenylacetaldehyde
0.0007 - 0.65
p-hydroxyphenylglycolaldehyde
0.141 - 0.15
p-Methoxybenzaldehyde
0.041
p-methylbenzaldehyde
-
-
10
p-nitroacetophenone
-
-
0.0013 - 5.9
p-nitrobenzaldehyde
0.091
phenylacetaldehyde
-
80°C, pH not specified in the publication
2.2
Phenylethanol
-
pH 10.0, 25°C
0.008 - 14.54
propionaldehyde
0.042 - 3
pyridine 3-aldehyde
4 - 5.2
pyridine 4-aldehyde
1.2 - 17.75
Pyruvaldehyde
0.066 - 6.9
Salicylaldehyde
0.126
sinapylaldehyde
-
-
2.95
sodium D-glucuronate
0.028 - 18
Succinic semialdehyde
0.06 - 27.1
trans-2-hexenal
0.22 - 1.69
trans-2-hexenol
0.058 - 0.073
veratraldehyde
additional information
additional information
-
5.4
(2S,5S)-hexanediol
-
wild type enzyme, at 70°C
327.6
(2S,5S)-hexanediol
-
mutant enzyme R11L/A180V, at 70°C
0.46
1,2-Cyclohexanedione
-
AR3
0.8
1,2-Cyclohexanedione
-
AR2
0.00251
1-Hexanol
-
-
6.79
1-Hexanol
-
pH 8.8, 30°C
0.00273
1-Pentanol
-
-
0.99
1-Pentanol
-
pH 10.0, 25°C
2.9
1-phenylethanol
-
cofactor NADP+, oxidation, recombinate wild-type, 30°C, pH 7.0
10.8
1-phenylethanol
-
NAD+, oxidation, recombinante mutant G37D, 30°C, pH 7.0
55.5
1-phenylethanol
-
cofactor NADP+, oxidation, recombinate mutant G37D, 30°C, pH 7.0
56.4
2,5-hexanedione
-
wild type enzyme, at 70°C
79
2,5-hexanedione
-
mutant enzyme R11L/A180V, at 70°C
0.38
2-butanol
cosubstrate: NADP+, pH 7.5, 80°C
7
2-butanol
-
r-TBADH, wild-type, mutant D150C-TBADH
7.17
2-butanol
-
mutant H59Q-TBADH
9.2
2-butanol
-
mutant C37A-TBADH
0.85
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant S196A/F197W
-
1.55
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant S196A
-
1.59
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant F197W
-
1.9
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant V84I
-
2.23
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant wild-type enzyme
-
2.4
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant V127C
-
4.39
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant V84I/Y127M
-
4.7
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant V84I/Y127C
-
5.9
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant V127M
-
6.38
2-hydroxytetrahydrofuran
-
pH 9.5, 30°C, recombinant mutant Y127C/S196A
-
0.049
2-Methoxybenzaldehyde
-
-
4.8
2-Methoxybenzaldehyde
-
pH 6.5, 30°C
1.85
2-methylbutanal
-
coenzyme NADPH
17.7
2-methylbutanal
-
coenzyme NADH
30.3
2-Pentanol
-
wild type enzyme, at 70°C
51.1
2-Pentanol
-
mutant enzyme R11L/A180V, at 70°C
60.8
2-Pentanol
pH 10.0, 70°C
0.408
2-propanol
-
-
168
2-propanol
pH 10.5, 60°C
1.61
3-methyl-1-butanol
-
pH 10.0, 25°C
4.6
3-methyl-1-butanol
-
pH 7.0, 25°C
0.048
3-Methylbutanal
-
pH 7.0, 25°C
0.129
3-Methylbutanal
-
pH 7.0, 25°C
0.21
3-Methylbutanal
-
coenzyme NADPH
1.89
3-Methylbutanal
-
coenzyme NADH
0.008
3-Pyridinecarboxaldehyde
-
AR3
0.02
3-Pyridinecarboxaldehyde
-
AR I
0.29
3-Pyridinecarboxaldehyde
-
aldehyde reductase II
0.606
3-Pyridinecarboxaldehyde
-
aldehyde reductase II
0.751
3-Pyridinecarboxaldehyde
-
G2
0.934
3-Pyridinecarboxaldehyde
-
AR1
1.18
3-Pyridinecarboxaldehyde
-
aldehyde reductase I
1.5
3-Pyridinecarboxaldehyde
-
-
2.4
3-Pyridinecarboxaldehyde
-
-
2.6
3-Pyridinecarboxaldehyde
-
-
2.9
3-Pyridinecarboxaldehyde
-
AR3
5
3-Pyridinecarboxaldehyde
-
AR4
8.61
3-Pyridinecarboxaldehyde
-
AR II
0.062
4-Pyridinecarboxaldehyde
-
AR2
0.69
4-Pyridinecarboxaldehyde
-
-
1.2
4-Pyridinecarboxaldehyde
-
enzyme from kidney, brain or liver
0.11
5alpha-dihydrotestosterone
-
AR2
4
5alpha-dihydrotestosterone
-
AR3
0.12 - 0.13
acetaldehyde
-
-
0.17
acetaldehyde
-
80°C, pH not specified in the publication
0.22
acetaldehyde
pH 8.0, 25°C, mutant G225A/A274F
0.25
acetaldehyde
-
1° Adh
0.25
acetaldehyde
-
pH 8.0, 80°C
0.51
acetaldehyde
-
in 30 mM Tris-HCl (pH 8.0)
0.84
acetaldehyde
recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
1
acetaldehyde
native enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
1.58
acetaldehyde
pH 8.0, 25°C, mutant A274F
2.1
acetaldehyde
-
2° Adh
2.1
acetaldehyde
pH 8.0, 25°C, mutant G225A
2.25
acetaldehyde
pH 8, 60°C
2.33
acetaldehyde
pH 8.0, 25°C, wild-type
3.1
acetaldehyde
-
pH 8.0, 80°C
10
acetaldehyde
-
isoform AdhA, pH 8.0, 60°C
28.47
acetaldehyde
-
pH 7.0, 37°C
44.8
acetaldehyde
-
secondary alcohol dehydrogenase
55.8
acetaldehyde
-
isoform AdhB, pH 8.0, 60°C
210
acetaldehyde
-
primary alcohol dehydrogenase
0.7
acetone
-
pH 7.8, 40°C
0.034
acetophenone
-
-
2.8
acetophenone
-
cofactor NADPH, reduction, recombinante wild-type, 30°C, pH 7.0
10.9
acetophenone
-
cofactor NADH, reduction, recombinante mutant G37D, 30°C, pH 7.0
0.099
androstane-3,17-dione
-
AR2
13
androstane-3,17-dione
-
AR3
0.01
benzaldehyde
-
co-substrate NADP
0.023
benzaldehyde
-
aldehyde reductase I
9.09
benzaldehyde
-
aldehyde reductase II
1.43
benzyl alcohol
-
-
2
benzyl alcohol
-
co-substrate NADP
10
butan-1-ol
-
2° Adh
43.75
butan-1-ol
pH 7.0. 30°C
0.31
butan-2-ol
-
pH 7.8, 40°C
0.51
butan-2-ol
-
2° Adh
0.11
Butanal
-
-
0.11
Butanal
-
in 30 mM Tris-HCl (pH 8.0)
2.76
Butanal
-
coenzyme NADPH
6.8
Butanal
pH 7.0, temperature not specified in the publication
23.1
Butanal
-
coenzyme NADH
1.4
butanol
-
-
1.9
butanol
-
pH 11.0, 80°C
3.28
butanol
-
in 30 mM Tris-HCl (pH 8.0)
0.12
Butanone
-
pH 7.8, 40°C
0.083
Butyraldehyde
-
aldehyde reductase I
0.45
Butyraldehyde
-
pH 8.0, 80°C
7.69
Butyraldehyde
-
aldehyde reductase II
0.017
cinnamaldehyde
-
-
0.017
cinnamaldehyde
-
in 30 mM Tris-HCl (pH 8.0)
0.043
cinnamaldehyde
-
pH 7.0, 25°C
0.172
cinnamaldehyde
-
pH 7.0, 25°C
0.08
cinnamyl alcohol
-
pH 10.0, 25°C
0.11
cinnamyl alcohol
-
pH 7.0, 25°C
0.27
cinnamyl alcohol
-
-
0.27
cinnamyl alcohol
-
in 30 mM Tris-HCl (pH 8.0)
0.436
cinnamyl alcohol
-
pH 7.0, 25°C
0.7
cinnamyl alcohol
-
pH 10.0, 25°C
1.18
cyclohexanone
-
G2
36.77
cyclohexanone
-
AR4
8.45
Cyclopentanol
-
r-TBADH, wild-type
8.7
Cyclopentanol
-
mutant H59Q-TBADH
16.71
Cyclopentanol
-
mutant D150C-TBADH
19.33
Cyclopentanol
-
mutant C37S-TBADH
1.5
D-glucuronate
-
-
2.5
D-glucuronate
-
outer medulla enzyme
3.22
D-glucuronate
-
inner medulla enzyme
3.34
D-glucuronate
-
cortex enzyme
4.2
D-glucuronate
-
wild-type aldehyde reductase
4.2
D-glucuronate
wild-type aldehyde reductase
4.2
D-glucuronate
-
aldehyde reductase, mutant V299C
7.5
D-glucuronate
aldehyde reductase, mutant R308A
19.5
D-glucuronate
-
aldehyde reductase, mutant I298C
26.3
D-glucuronate
-
aldehyde reductase, mutant I298A
42
D-glucuronate
-
enzyme from brain
43
D-glucuronate
-
enzyme from kidney, or liver
48
D-glucuronate
-
aldehyde reductase, mutant H112Q
364
D-glucuronate
aldehyde reductase, mutant R311A
1.29
D-glucuronic acid
-
AR1
2.48
D-glucuronic acid
-
G2
2.99
D-glucuronic acid
-
AR3
19.6
D-glucuronolactone
-
AR2
67
D-glucuronolactone
-
AR1
0.43
D-glyceraldehyde
-
AR2
8.2
D-glyceraldehyde
-
AR3
54.4
D-xylose
pH 10.5, 60°C
500
D-xylose
-
aldehyde reductase II
845
D-xylose
-
outer medulla enzyme
1152
D-xylose
-
inner medulla enzyme
1497
D-xylose
-
cortex enzyme
0.21
diacetyl
cosubstrate: NADPH, pH 7.5, 80°C
1.3
DL-glyceraldehyde
-
-
1.5
DL-glyceraldehyde
-
-
1.5
DL-glyceraldehyde
-
-
1.7
DL-glyceraldehyde
-
wild-type aldehyde reductase
1.7
DL-glyceraldehyde
wild-type aldehyde reductase
1.7
DL-glyceraldehyde
-
wild type enzyme, at pH 7.0 and 25°C
1.7
DL-glyceraldehyde
wild type enzyme, at pH 7.0 and 25°C
1.8
DL-glyceraldehyde
-
aldehyde reductase, mutant V299C
1.8
DL-glyceraldehyde
-
mutant enzyme V299C, at pH 7.0 and 25°C
2.2
DL-glyceraldehyde
aldehyde reductase, mutant R311A
2.2
DL-glyceraldehyde
mutant enzyme R311A, at pH 7.0 and 25°C
2.3
DL-glyceraldehyde
aldehyde reductase, mutant R308A
2.3
DL-glyceraldehyde
mutant enzyme R308A, at pH 7.0 and 25°C
2.94
DL-glyceraldehyde
-
aldehyde reductase II
2.94
DL-glyceraldehyde
-
aldehyde reductase II, at pH 7.0 and 37°C
4.67
DL-glyceraldehyde
-
inner medulla enzyme
4.67
DL-glyceraldehyde
-
enzyme from inner medulla, at pH 6.2 and 25°C
4.68
DL-glyceraldehyde
-
cortex enzyme
4.68
DL-glyceraldehyde
-
enzyme from brain cortex, at pH 6.2 and 25°C
5.92
DL-glyceraldehyde
-
outer medulla enzyme
5.92
DL-glyceraldehyde
-
enzyme from outer medulla, at pH 6.2 and 25°C
8.35
DL-glyceraldehyde
-
-
11.5
DL-glyceraldehyde
-
aldehyde reductase, mutant I298C
11.5
DL-glyceraldehyde
-
mutant enzyme I298C, at pH 7.0 and 25°C
12.5
DL-glyceraldehyde
-
aldehyde reductase I
12.5
DL-glyceraldehyde
-
aldehyde reductase I, at pH 7.3 and 37°C
33
DL-glyceraldehyde
-
aldehyde reductase, mutant I298A
33
DL-glyceraldehyde
-
mutant enzyme I298A, at pH 7.0 and 25°C
76
DL-glyceraldehyde
-
aldehyde reductase, mutant H112Q
76
DL-glyceraldehyde
-
mutant enzyme H112Q, at pH 7.0 and 25°C
0.013
ethanol
-
NADP+
0.5
ethanol
pH 8.0, 25°C, wild-type
0.74
ethanol
pH 8.0, 25°C, mutant G225A
0.85
ethanol
pH 8.0, 25°C, mutant G225A/A274F
1.02
ethanol
pH 8.0, 25°C, mutant A274F
8
ethanol
-
pH 8.0, 80°C
9.7
ethanol
-
pH 11.0, 80°C
9.7
ethanol
recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
10.4
ethanol
native enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
16.4
ethanol
-
primary alcohol dehydrogenase
18.8
ethanol
-
in 30 mM Tris-HCl (pH 8.0)
29.4
ethanol
-
80°C, pH not specified in the publication
40
ethanol
-
isoform AdhA, pH 8.0, 60°C
49
ethanol
-
secondary alcohol dehydrogenase
24
ethyl acetoacetate
-
enzyme from brain or liver
25
ethyl acetoacetate
-
enzyme from kidney
2.7
ethylacetoacetate
-
AR2
26
ethylacetoacetate
-
AR3
2.5
glucuronate
-
aldehyde reductase II
0.07
glyceraldehyde
-
AR I
0.07
glyceraldehyde
-
aldehyde reductase I, at pH 7.3 and 37°C
6.67
glyceraldehyde
-
AR II
6.67
glyceraldehyde
-
aldehyde reductase II, at pH 7.0 and 37°C
7.59
glyceraldehyde
-
aldehyde reductase II
0.27
Heptanal
-
coenzyme NADPH
4.25
Heptanal
-
coenzyme NADH
0.04
hexanal
-
-
0.152
hexanal
-
pH 7.0, 25°C
0.18
hexanal
-
coenzyme NADPH
0.83
hexanal
-
coenzyme NADH
5.5
hexanal
-
pH 6.5, 30°C
0.35
Hexanol
-
-
1.22
Hexanol
-
coenzyme NADPH
9.1
Hexanol
-
pH 7.0, 25°C
0.0059
indol-3-ylacetaldehyde
-
low-Km reductase
0.2
indol-3-ylacetaldehyde
-
high-Km aldehyde reductase
0.32
Indole-3-acetaldehyde
-
AR2
3
Indole-3-acetaldehyde
-
AR3
3.7
Indole-3-acetaldehyde
-
AR1
4.9
L-glyceraldehyde
-
AR2
11.1
L-glyceraldehyde
-
AR3
0.027
m-methoxybenzaldehyde
-
-
0.212
m-methoxybenzaldehyde
-
-
0.002
m-nitrobenzaldehyde
-
-
0.0029
m-nitrobenzaldehyde
-
-
0.082
m-nitrobenzaldehyde
-
AR2
2.4
m-nitrobenzaldehyde
-
-
3.9
m-nitrobenzaldehyde
-
AR1
4
m-nitrobenzaldehyde
-
AR3
4.4
m-nitrobenzaldehyde
-
-
0.009
menadione
-
AR4
1.2
methyl 4-bromo-3-oxobutanoate
-
wild-type, pH 6.5, 30°C
1.2
methyl 4-bromo-3-oxobutanoate
-
mutant L54Q, pH 6.5, 30°C
0.2
methylglyoxal
-
80°C, pH not specified in the publication
0.0033
n-butanol
-
-
47.1
n-butanol
-
primary alcohol dehydrogenase
1.1
n-Butyraldehyde
-
AR2
0.019
n-dodecanal
-
enzyme from brain
0.02
n-dodecanal
-
enzyme from liver and kidney
12
n-Propanol
-
-
0.096
NAD+
-
1° Adh, ethanol as substrate
0.2
NAD+
-
ethanol as substrate
0.39
NAD+
pH 8.0, 25°C, wild-type
0.39
NAD+
wild type enzyme, at 25°C, pH not specified in the publication
0.57
NAD+
pH 8.0, 25°C, mutant G225A/A274F
0.57
NAD+
mutant enzyme G225A/A274F, at 25°C, pH not specified in the publication
0.75
NAD+
-
reduction, recombinant mutant G37D, 30°C, pH 7.0
0.8
NAD+
-
octanol as substrate
0.98
NAD+
pH 8.0, 25°C, mutant A274F
0.98
NAD+
mutant enzyme A274F, at 25°C, pH not specified in the publication
1
NAD+
pH 8.0, 25°C, mutant G225A
1
NAD+
mutant enzyme G225A, at 25°C, pH not specified in the publication
1.44
NAD+
cosubstrate octan-1-ol, pH 8, 60°C
9.7
NAD+
-
in 30 mM Tris-HCl (pH 8.0)
20
NAD+
-
80°C, pH not specified in the publication
0.025
NADH
-
1° Adh, acetaldehyde as substrate
0.025
NADH
pH 8.0, 25°C, wild-type
0.025
NADH
wild type enzyme, at 25°C, pH not specified in the publication
0.045
NADH
pH 8.0, 25°C, mutant G225A
0.045
NADH
mutant enzyme G225A, at 25°C, pH not specified in the publication
0.052
NADH
-
aldehyde reductase 2, at pH 6.0 and 25°C
0.06
NADH
pH 8.0, 25°C, mutant G225A/A274F
0.06
NADH
mutant enzyme G225A/A274F, at 25°C, pH not specified in the publication
0.07
NADH
pH 8.0, 25°C, mutant A274F
0.07
NADH
mutant enzyme A274F, at 25°C, pH not specified in the publication
0.52
NADH
-
m-nitrobenzaldehyde as substrate
0.84
NADH
cosubstrate octanal, pH 8, 60°C
1.31
NADH
-
in 30 mM Tris-HCl (pH 8.0)
1.6
NADH
-
oxidation, recombinante mutant G37D, 30°C, pH 7.0
20.55
NADH
substrate furfural, pH 7.2, 30°C
23.84
NADH
substrate furfural, pH 7.2, 30°C
0.007
NADP+
-
-
0.013
NADP+
-
pH 10.0, 25°C
0.015
NADP+
-
reduction, recombinant wild-type, 30°C, pH 7.0
0.02
NADP+
-
1° Adh, ethanol as substrate
0.02
NADP+
-
pH 11.0, 80°C
0.022
NADP+
-
secondary alcohol dehydrogenase
0.022
NADP+
pH 10.5, 60°C
0.049
NADP+
-
2° Adh, butan-2-ol as substrate
0.05
NADP+
-
in 30 mM Tris-HCl (pH 8.0)
0.06
NADP+
-
ethanol as substrate
0.061
NADP+
-
pH 10.0, 25°C
0.0648
NADP+
-
pH 9.5, 30°C, recombinant wild-type enzyme, with 2-hydroxytetrahydrofuran
0.07
NADP+
-
2° Adh, ethanol as substrate
0.071
NADP+
-
80°C, pH not specified in the publication
0.0768
NADP+
-
pH 9.5, 30°C, recombinant mutant V127M, with 2-hydroxytetrahydrofuran
0.08
NADP+
pH 10.0, 70°C
0.0807
NADP+
-
pH 9.5, 30°C, recombinant mutant V84I/Y127M, with 2-hydroxytetrahydrofuran
0.1
NADP+
-
pH 9.5, 30°C, recombinant mutant S196A/F197W, with 2-hydroxytetrahydrofuran
0.107
NADP+
-
pH 9.5, 30°C, recombinant mutant F197W, with 2-hydroxytetrahydrofuran
0.107
NADP+
-
pH 9.5, 30°C, recombinant mutant V127C, with 2-hydroxytetrahydrofuran
0.15
NADP+
-
primary alcohol dehydrogenase
0.159
NADP+
-
pH 9.5, 30°C, recombinant mutant V84I, with 2-hydroxytetrahydrofuran
0.2
NADP+
-
wild type enzyme, at 70°C
0.35
NADP+
-
secondary alcohol dehydrogenase
0.4
NADP+
cosubstrate: 2-butanol, pH 7.5, 80°C
0.456
NADP+
-
pH 9.5, 30°C, recombinant mutant S196A, with 2-hydroxytetrahydrofuran
0.462
NADP+
-
pH 9.5, 30°C, recombinant mutant V84I/Y127C, with 2-hydroxytetrahydrofuran
0.565
NADP+
-
pH 9.5, 30°C, recombinant mutant Y127C/S196A, with 2-hydroxytetrahydrofuran
0.7
NADP+
-
mutant enzyme R11L/A180V, at 70°C
0.8
NADP+
-
benzyl aclohol as substrate
0.83
NADP+
-
primary alcohol dehydrogenase
2.4
NADP+
cosubstrate octan-1-ol, pH 8, 60°C
2.64
NADP+
pH 8.0, 25°C, wild-type
2.77
NADP+
pH 8.0, 25°C, mutant G225A
5.5
NADP+
pH 8.0, 25°C, mutant G225A/A274F
6.21
NADP+
pH 8.0, 25°C, mutant A274F
6.3
NADP+
-
pH 8.0, 80°C
9.1
NADP+
-
reduction, recombinant mutant G37D, 30°C, pH 7.0
9.7
NADP+
recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
10.4
NADP+
native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
26.88
NADP+
pH 7.0. 30°C
46.7
NADP+
-
pH 8.8, 30°C
0.001
NADPH
-
-
0.001
NADPH
-
p-nitrobenzaldehyde as substrate
0.0012
NADPH
-
low-Km reductase
0.0016
NADPH
-
pyridine 3-aldehyde as substrate
0.0019
NADPH
-
D-glucuronate as substrate
0.004
NADPH
-
n-propanol as substrate
0.005
NADPH
-
benzaldehyde as substrate
0.006
NADPH
-
pH 8.0, 80°C
0.006
NADPH
recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
0.007
NADPH
native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)
0.011
NADPH
-
pH 7.0, 25°C
0.011
NADPH
cosubstrate: 2-butanone, pH 7.5, 80°C
0.012
NADPH
-
m-nitrobenzaldehyde as substrate
0.013
NADPH
-
aldehyde reductase II
0.014
NADPH
-
propionaldehyde as substrate
0.014
NADPH
-
pH 8.0, 80°C
0.019
NADPH
-
aldehyde reductase II
0.021
NADPH
-
benzyl alcohol as substrate
0.022
NADPH
-
aldehyde reductase I
0.025
NADPH
-
in 30 mM Tris-HCl (pH 8.0)
0.029
NADPH
-
pH 7.0, 25°C
0.033
NADPH
-
2° Adh, propanone as substrate
0.04
NADPH
-
oxidation, recombinante wild-typ, 30°C, pH 7.0
0.05
NADPH
pH 8.0, 25°C, mutant G225A/A274F
0.055
NADPH
-
2° Adh, acetaldehyde as substrate
0.071
NADPH
-
80°C, pH not specified in the publication
0.094
NADPH
-
1°Adh, acetaldehyde as substrate
0.1
NADPH
-
mutant enzyme R11L/A180V, at 70°C
0.1
NADPH
pH 7.0, temperature not specified in the publication
0.13
NADPH
-
benzaldehyde as substrate
0.21
NADPH
pH 8.0, 25°C, wild-type
0.24
NADPH
substrate furfural, pH 7.2, 30°C
0.25
NADPH
-
high-Km aldehyde reductase
0.26
NADPH
substrate 5-hydroxymethylfurfural, pH 7.2, 30°C
0.36
NADPH
-
secondary alcohol dehydrogenase
0.37
NADPH
pH 8.0, 25°C, mutant A274F
0.37
NADPH
pH 8.0, 25°C, mutant G225A
0.4
NADPH
-
wild type enzyme, at 70°C
1.5
NADPH
-
primary alcohol dehydrogenase
4.11
NADPH
substrate furfural, pH 7.2, 30°C
0.376
o-nitrobenzaldehyde
-
-
1.3
o-nitrobenzaldehyde
-
-
2.8
o-nitrobenzaldehyde
-
AR2
4.6
o-nitrobenzaldehyde
-
AR1
4.6
o-nitrobenzaldehyde
-
AR3
0.01
octanal
-
-
0.09
Octanol
-
-
0.004
p-Carboxybenzaldehyde
-
-
0.018
p-Carboxybenzaldehyde
-
AR1
0.025
p-Carboxybenzaldehyde
-
-
0.031
p-Carboxybenzaldehyde
-
-
0.038
p-Carboxybenzaldehyde
-
wild-type aldehyde reductase
0.038
p-Carboxybenzaldehyde
wild-type aldehyde reductase
0.05
p-Carboxybenzaldehyde
-
-
0.056
p-Carboxybenzaldehyde
-
AR1
0.062
p-Carboxybenzaldehyde
-
AR3
0.078
p-Carboxybenzaldehyde
-
-
0.1
p-Carboxybenzaldehyde
-
aldehyde reductase, mutant V299C
0.11
p-Carboxybenzaldehyde
aldehyde reductase, mutant R308A
0.258
p-Carboxybenzaldehyde
-
AR3
0.32
p-Carboxybenzaldehyde
-
AR2
0.38
p-Carboxybenzaldehyde
-
aldehyde reductase, mutant I298A
0.43
p-Carboxybenzaldehyde
-
aldehyde reductase, mutant I298C
2.8
p-Carboxybenzaldehyde
-
aldehyde reductase, mutant H112Q
4
p-Carboxybenzaldehyde
aldehyde reductase, mutant R311A
0.94
p-chlorobenzaldehyde
-
-
6.7
p-chlorobenzaldehyde
-
-
0.005
p-cyanobenzaldehyde
-
-
0.51
p-cyanobenzaldehyde
-
-
0.0023
p-hydroxyphenylacetaldehyde
-
low-Km reductase
0.057
p-hydroxyphenylacetaldehyde
-
-
0.85
p-hydroxyphenylacetaldehyde
-
high-Km aldehyde reductase
0.91
p-hydroxyphenylacetaldehyde
-
-
0.0007
p-hydroxyphenylglycolaldehyde
-
low-Km reductase
0.016
p-hydroxyphenylglycolaldehyde
-
-
0.059
p-hydroxyphenylglycolaldehyde
-
-
0.65
p-hydroxyphenylglycolaldehyde
-
high-Km aldehyde reductase
0.141
p-Methoxybenzaldehyde
-
-
0.15
p-Methoxybenzaldehyde
-
-
0.0013
p-nitrobenzaldehyde
-
low-Km reductase
0.0026
p-nitrobenzaldehyde
-
low-Km reductase
0.004
p-nitrobenzaldehyde
-
low-Km aldehyde reductase
0.006
p-nitrobenzaldehyde
-
-
0.0084
p-nitrobenzaldehyde
-
AR3
0.029
p-nitrobenzaldehyde
-
aldehyde reductase I
0.034
p-nitrobenzaldehyde
-
AR2
0.041
p-nitrobenzaldehyde
-
high-Km reductase
0.044
p-nitrobenzaldehyde
-
-
0.071
p-nitrobenzaldehyde
-
AR1
0.08
p-nitrobenzaldehyde
-
-
0.12
p-nitrobenzaldehyde
-
-
0.129
p-nitrobenzaldehyde
-
G2
0.15
p-nitrobenzaldehyde
-
-
0.16
p-nitrobenzaldehyde
-
wild-type aldehyde reductase
0.16
p-nitrobenzaldehyde
wild-type aldehyde reductase
0.169
p-nitrobenzaldehyde
-
aldehyde reductase II
0.2
p-nitrobenzaldehyde
-
enzyme from kidney, brain or liver
0.23
p-nitrobenzaldehyde
-
-
0.25
p-nitrobenzaldehyde
aldehyde reductase, mutant R308A
0.25
p-nitrobenzaldehyde
-
high-Km aldehyde reductase
0.262
p-nitrobenzaldehyde
-
AR4
0.27
p-nitrobenzaldehyde
-
-
0.29
p-nitrobenzaldehyde
-
high-Km aldehyde reductase
0.31
p-nitrobenzaldehyde
-
-
0.35
p-nitrobenzaldehyde
-
-
0.38
p-nitrobenzaldehyde
-
aldehyde reductase, mutant V299C
0.38
p-nitrobenzaldehyde
mutant R311A
0.8
p-nitrobenzaldehyde
-
-
1.1
p-nitrobenzaldehyde
-
AR3
1.2
p-nitrobenzaldehyde
-
-
1.2
p-nitrobenzaldehyde
-
AR1
1.2
p-nitrobenzaldehyde
-
aldehyde reductase, mutant I298C
2.8
p-nitrobenzaldehyde
-
aldehyde reductase, mutant I298A
5.9
p-nitrobenzaldehyde
-
aldehyde reductase, mutant H112Q
0.049
pentanal
-
pH 7.0, 25°C
0.06
pentanal
-
pH 7.0, 25°C
0.075
pentanal
-
in 30 mM Tris-HCl (pH 8.0)
0.16
pentanal
-
coenzyme NADPH
0.4
pentanal
pH 7.0, temperature not specified in the publication
3.01
pentanal
-
coenzyme NADH
0.22
Pentanol
-
at pH 10
1.54
Pentanol
-
in 30 mM Tris-HCl (pH 8.0)
0.11
Phenylglyoxal
-
AR2
0.56
propan-2-ol
-
2° Adh
16.4
propan-2-ol
-
secondary alcohol dehydrogenase
27
propanal
-
coenzyme NADH
38.9
propanal
-
coenzyme NADPH
0.008
propionaldehyde
-
aldehyde reductase I
0.087
propionaldehyde
-
-
0.21
propionaldehyde
-
AR I
2.63
propionaldehyde
-
AR II
3.31
propionaldehyde
-
pH 7.0, 37°C
14.28
propionaldehyde
-
aldehyde reductase II
14.54
propionaldehyde
-
aldehyde reductase II
0.042
pyridine 3-aldehyde
-
AR2
0.26
pyridine 3-aldehyde
-
-
3
pyridine 3-aldehyde
-
AR1
4
pyridine 4-aldehyde
-
AR1
5.2
pyridine 4-aldehyde
-
AR3
1.2
Pyruvaldehyde
-
wild type enzyme, at 70°C
3.3
Pyruvaldehyde
pH 7.0, 70°C
4.2
Pyruvaldehyde
-
mutant enzyme R11L/A180V, at 70°C
17.75
Pyruvaldehyde
pH 7.5, 60°C
0.066
Salicylaldehyde
-
-
6.9
Salicylaldehyde
-
pH 6.5, 30°C
2.95
sodium D-glucuronate
-
aldehyde reductase II
2.95
sodium D-glucuronate
-
aldehyde reductase II, at pH 7.0 and 37°C
0.028
Succinic semialdehyde
-
AR4
0.029
Succinic semialdehyde
-
AR1
0.036
Succinic semialdehyde
-
G2
0.037
Succinic semialdehyde
-
-
0.064
Succinic semialdehyde
-
AR3
0.1
Succinic semialdehyde
-
reductase I
0.13
Succinic semialdehyde
-
-
0.17
Succinic semialdehyde
-
wild-type aldehyde reductase
0.17
Succinic semialdehyde
wild-type aldehyde reductase
0.2
Succinic semialdehyde
aldehyde reductase, mutant R308A
0.41
Succinic semialdehyde
-
aldehyde reductase, mutant V299C
1.5
Succinic semialdehyde
-
aldehyde reductase, mutant I298C
2.8
Succinic semialdehyde
-
aldehyde reductase, mutant I298A
10.9
Succinic semialdehyde
-
liver enzyme
14.1
Succinic semialdehyde
aldehyde reductase, mutant R311A
18
Succinic semialdehyde
-
aldehyde reductase, mutant H112Q
0.06
trans-2-hexenal
-
-
27.1
trans-2-hexenal
-
pH 6.5, 30°C
0.22
trans-2-hexenol
-
-
1.69
trans-2-hexenol
-
coenzyme NADPH
0.058
veratraldehyde
-
pH 7.0, 25°C
0.073
veratraldehyde
-
pH 7.0, 25°C
41
xylose
-
AR3
additional information
additional information
-
kinetic analysis of recombinant wild-type an d mutant enzymes, overview
-
additional information
additional information
-
kinetic analysis of wild-type and mutant enzymes, fit to the ordered bi-bi rate equation, steady-state kinetics, detailed overview. For some mutants (G211S, G211C, G211InsA and G211InsS), with NAD+ as a cofactor, the KA or KB values are observed to be higher than the maximum concentration of substrate or cofactor utilized. So, for mutants G211S, G211C, G211InsA and G211InsS, kinetic data is also measured using assay reaction mixtures containing 1-450 mM 2,3-butanediol and 0.001-5.50 mM NAD+
-