1.1.1.12: L-arabinitol 4-dehydrogenase

This is an abbreviated version!
For detailed information about L-arabinitol 4-dehydrogenase, go to the full flat file.

Reaction

Synonyms

ADH, dehydrogenase, L-arabinitol, HjLAD, L-arabinitol dehydrogenase, L-arabitol dehydrogenase, LAD, Lad1, LADA, More, NAD(P)+-dependent arabitol dehydrogenase, pentitol-DPN dehydrogenase, XYL2

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.12 L-arabinitol 4-dehydrogenase

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Results	in table
24	AA Sequence
7	Application
1	CAS Registry Number
13	Cloned(Commentary)
37	Cofactor
3	Crystallization (Commentary)
2	Expression
22	General Information
11	Inhibitors
25	kcat/KM [mM/s]
74	KM Value [mM]
2	Localization
10	Metals/Ions
9	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
50	Organism
3	Pathway
2	PDB ID
9	pH Optimum
7	pH Range
26	Protein Variants
10	Purification (Commentary)
1	Reaction
3	Reaction Type
44	Reference
4	Source Tissue
13	Specific Activity [micromol/min/mg]
4	Storage Stability
80	Substrates and Products (Substrate)
11	Subunits
54	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
4	Temperature Range [°C]
7	Temperature Stability [°C]
42	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.12 - L-arabinitol 4-dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Neurospora crassa

Q7SI09

712767

native enzyme by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interation chromatography, and another different step of anion exchange chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography

Meyerozyma caribbica

A0A1B4XTS0

738728

native enzyme by dialysis, anion exchange chromatography, ammoniuim sulfate fractionation, hydrophobic interaction chromatography, again dialysis and another different step off anion exchange chromatography, dialysis, and gel filtration, followed by repeating the last anion exchange chromatographic step. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography

Meyerozyma caribbica

A0A1B4XTS0

761551

partial

Aspergillus niger

-

668063

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)

Trichoderma reesei

Q96V44

739939

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Trichoderma reesei

Q96V44

762388

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)

Trichoderma reesei

Q96V44

740145

recombinant protein

Neurospora crassa

-

684627

recombinant protein, expression in Escherichia coli

Trichoderma reesei

-

655480

recombinant proteins purified using Ni-agarose

Aspergillus niger

A2QAC0

696988