1.1.1.103: L-threonine 3-dehydrogenase
This is an abbreviated version!
For detailed information about L-threonine 3-dehydrogenase, go to the full flat file.
Reaction
Synonyms
CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase
ECTree
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Metals Ions
Metals Ions on EC 1.1.1.103 - L-threonine 3-dehydrogenase
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Ca2+
Cd2+
Co2+
Cu2+
Mg2+
Mn2+
Zn2+
additional information
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preincubation with 0.07 or 5 mM leads to 3fold increase of C38D mutant enzyme activity, 0.5 mM, 6.5fold increase of wild-type enzyme activity, substrate L-threonine
Cd2+
-
0.064 or 3.2 mM, 6fold increase of L-threonine dehydrogenase activity
Mn2+
-
preincubation with 1 mM, 3.5fold increase of L-threonine oxidation
Mn2+
-
0.064 or 3.2 mM, 4fold increase of L-threonine dehydrogenase activity, activation is dependent on the presence of a reduced thiol in all enzyme stock solutions and assay buffers, binding of 0.86 mol of Mn2+ per mol of enzyme subunit
Zn2+
-
5 mM, 60fold increase of C38D mutant enzyme activity, Zn2+ stimulated activity decreases to 10% when 1 mM Mn2+ is added, no increase in activity for wild-type enzyme
Zn2+
-
exchange of Zn2+ with Co2+ or Cd2+ does not change the specific activity of the enzyme
Zn2+
-
2 Zn2+ per enzyme subunit, binding involves residues G168, G175, G180, and G212, overview
Zn2+
1.22 mol of Zn2+ per mol of enzyme subunit, the catalytic zinc atom at the active center of TDH is coordinated by the highly conserved four residues Cys42, His67, Glu68 and Glu152 with low affinity
Zn2+
one molecule of TDH contains one zinc ion playing a structural role, the metal ion is ligated by foru Cys residues, coenzyme-binding domain shows a larger interdomain cleft compared to other ADHs, binding structure, overview
Zn2+
-
four zinc-binding cysteine residues, 0.1 mM reactivates EDTA-100% inhibited enzyme by 75%, 0.05 mM reactivates by 100%
no effect at 1 mM by AgNO3, MgCl2, and 10 mM EDTA and ethylene glycol tetraacetic acid
additional information
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no effect at 1 mM by AgNO3, MgCl2, and 10 mM EDTA and ethylene glycol tetraacetic acid
additional information
the active site catalytic zinc ion is absent from the TDH structure
additional information
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the active site catalytic zinc ion is absent from the TDH structure