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0.00009
-
substrate: 3-oxohexanoyl-[acyl-carrier protein], pH and temperature not specified in the publication
0.0001
-
substrate: 3-oxobutyryl-[acyl-carrier protein], pH and temperature not specified in the publication
0.0017 - 0.0018
-
recombinant refolded enzyme
0.0019
-
recombinant soluble enzyme
0.00361
-
substrate: 3-oxooctanoyl-[acyl-carrier protein], pH and temperature not specified in the publication
0.00421
-
substrate: 3-oxohexadecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication
0.00456
-
substrate: 3-oxotetradecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication
0.00851
-
substrate: 3-oxododecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication
0.02
with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.06
mutant enzyme K173A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
0.08
with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.15
with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.16
with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.46
mutant enzyme K152A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
0.5
mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
0.65
mutant enzyme Y169A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
0.85
mutant enzyme Y169A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
1.03
with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
1.16
with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
1.2
mutant enzyme K173A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
1.3
mutant enzyme K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
1.6
mutant enzyme R168E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
1.7
mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
136000
-
purified enzyme, protein determination by calculation from absorption at 280 nm
14.8
mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
15.7
mutant enzyme K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
17.4
mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
180400
-
recombinant protein, cosubstrate NADPH, pH 7.0, phosphate buffer
19.3
mutant enzyme K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
2.4
mutant enzyme K173A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
2.45
with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
2.6
wild type enzyme, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
20
mutant enzyme D42A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
20.5
wild type enzyme, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
20.7
mutant enzyme R34A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
20.8
wild type enzyme, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
21
mutant enzyme R34A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
21.7
mutant enzyme R168E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
22
mutant enzyme K152A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
25.5
mutant enzyme K152A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
280
-
acetoacetyl-N-acetylcysteamine
3
-
mutant R187A/R230A, acyl-carrier protein-dependent spectroscopic assay
3.1
mutant enzyme Y169A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
3.5
wild type enzyme, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
3.93
with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
31.8
mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
38.29
with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
3839
-
recombinant protein, cosubstrate NADH, pH 7.0, phosphate buffer
4
-
mutant R230E, acyl-carrier protein-dependent spectroscopic assay
4.3
mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
4.5
mutant enzyme K173A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
42.6
-
mutant R187E/R230E, acyl-carrier protein-independent spectroscopic assay
45.6
-
mutant R187A/R230A, acyl-carrier protein-independent spectroscopic assay
5.4
mutant enzyme D42A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
5.5
mutant enzyme K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
54.2
-
mutant R230E, acyl-carrier protein-independent spectroscopic assay
54.5
-
mutant R187E, acyl-carrier protein-independent spectroscopic assay
55.3
-
mutant R230A, acyl-carrier protein-independent spectroscopic assay
57.9
-
mutant R187A, acyl-carrier protein-independent spectroscopic assay
6.1
-
mutant R230A, acyl-carrier protein-dependent spectroscopic assay
6.9
-
mutant R187A, acyl-carrier protein-dependent spectroscopic assay
7.3
mutant enzyme D42A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
7.9
mutant enzyme R168E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
70.6
-
wild-type, acyl-carrier protein-dependent spectroscopic assay
74.6
mutant enzyme D42A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
8.4
mutant enzyme Q126E/R168E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
83140
-
cosubstrate NADPH, pH 8.35, carbonate buffer
9401
-
purified enzyme, protein determination by amino acid analysis
0.0197
-
-
0.0197
-
substrate: 3-oxodecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication
0.13
with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.13
with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.44
mutant enzyme R34A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
0.44
mutant enzyme R34A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
2
mutant enzyme Y169A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
2
-
mutant R187E/R230E, acyl-carrier protein-dependent spectroscopic assay
59.8
-
purified recombinant enzyme
59.8
-
wild-type, acyl-carrier protein-independent spectroscopic assay
6.3
mutant enzyme R168E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
6.3
-
mutant R187E, acyl-carrier protein-dependent spectroscopic assay
additional information
the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639.
additional information
-
the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639.