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89.2
1,1,1-trifluoroacetone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
5.72
2',3',4',5',6'-pentafluoroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
7.38
3'-fluoroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.142
3-oxododecanoyl-[acyl-carrier protein]
-
pH and temperature not specified in the publication
0.6
3-oxooctanoyl-CoA
pH 7.0, 25°C
14.34
4'-chloroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
12.29
4'-fluoroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.0057 - 3.5
acetoacetyl-CoA
48
acetoacetyl-N-acetylcysteamine
-
-
0.003 - 0.017
acetoacetyl-[acyl-carrier protein]
46.92
acetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
2.71
alpha-chloroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.29 - 4.06
beta-ketobutyryl-CoA
8.31
ethyl 4-chloroacetoacetate
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.127
ethyl acetoacetate
-
-
1.92
ethylbenzoylacetate
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.035
N-(2-sulfanylethyl)acetamide
-
-
0.29
NADP+
with 3-hydroxydecanoyl-N-acetylcysteamine as cosubstrate, pH and temperature not specified in the publication
6.03
propiophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
additional information
additional information
-
0.0057
acetoacetyl-CoA
wild type enzyme, at pH 8.0 and 30°C
0.0136
acetoacetyl-CoA
mutant enzyme Q47L, at pH 8.0 and 30°C
0.0159
acetoacetyl-CoA
mutant enzyme Q47L, at pH 8.0 and 30°C
0.075
acetoacetyl-CoA
pH 7.5
0.165
acetoacetyl-CoA
-
pH 7.6, 30°C
0.261
acetoacetyl-CoA
-
-
0.43
acetoacetyl-CoA
-
wild-type
0.43
acetoacetyl-CoA
-
pH 6.8, 22°C, recombinant enzyme
0.44
acetoacetyl-CoA
-
mutant R230A
0.45
acetoacetyl-CoA
-
mutant R187A
0.47
acetoacetyl-CoA
-
mutant R187A/R230A
0.47
acetoacetyl-CoA
-
mutant R187E
0.49
acetoacetyl-CoA
-
mutant R230E
0.51
acetoacetyl-CoA
pH 6.8, 25°C, recombinant enzyme
0.58
acetoacetyl-CoA
-
mutant R187E/R230E
0.8158
acetoacetyl-CoA
wild type enzyme, at pH 8.0 and 25°C
0.8871
acetoacetyl-CoA
mutant enzyme S36A, at pH 8.0 and 25°C
0.9913
acetoacetyl-CoA
mutant enzyme S40A, at pH 8.0 and 25°C
1
acetoacetyl-CoA
pH and temperature not specified in the publication
2.2
acetoacetyl-CoA
-
pH 7.6, 30°C
3.5
acetoacetyl-CoA
pH 7.0, 25°C
0.003
acetoacetyl-[acyl-carrier protein]
-
-
0.0037
acetoacetyl-[acyl-carrier protein]
-
-
0.0079
acetoacetyl-[acyl-carrier protein]
-
-
0.009
acetoacetyl-[acyl-carrier protein]
-
-
0.017
acetoacetyl-[acyl-carrier protein]
-
-
0.29
beta-ketobutyryl-CoA
-
pH 7.0, 30°C, FabG1
4.06
beta-ketobutyryl-CoA
-
pH 7.0, 30°C, FabG2
0.0093
NADPH
-
-
0.0205
NADPH
wild type enzyme, at pH 8.0 and 25°C
0.023
NADPH
-
+ acetoacetyl-[acyl-carrier-protein]
0.026
NADPH
-
pH 7.6, 30°C
0.0264
NADPH
mutant enzyme S36A, at pH 8.0 and 25°C
0.0412
NADPH
-
in the presence of 1.5 M urea, pH 7.0, 25°C
0.043
NADPH
-
pH 6.8, 22°C, recombinant enzyme
0.0434
NADPH
-
in the presence of 1 M urea, pH 7.0, 25°C
0.0461
NADPH
-
without urea, pH 7.0, 25°C
0.0475
NADPH
pH 6.8, 25°C, recombinant enzyme
0.0571
NADPH
mutant enzyme S40A, at pH 8.0 and 25°C
0.149
NADPH
wild type enzyme, at pH 8.0 and 30°C
0.289
NADPH
mutant enzyme Q47L, at pH 8.0 and 30°C
0.29
NADPH
with acetoacetyl-CoA as cosubstrate, pH and temperature not specified in the publication
0.4553
NADPH
-
in the presence of 4 M urea, pH 7.0, 25°C
0.617
NADPH
mutant enzyme Q47L, at pH 8.0 and 30°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
cooperativity kinetics, binding constants of substrates and cofactors
-
additional information
additional information
-
detailed kinetic and thermodynamic analysis, solvent and multiple kinetic isotope effects, kinetic mechanism
-
additional information
additional information
-
detailed steady-state kinetic analysis, solvent and multiple kinetic isotope effects, random bi bi kinetic mechanism
-
additional information
additional information
kinetic analysis of the recombinant enzyme, kinetic mechanism
-
additional information
additional information
-
kinetic analysis of the recombinant enzyme, kinetic mechanism
-