EC Number   |
Protein Variants |
Reference |
|---|
   2.7.1.78 | D165A |
site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining |
642028 |
| 2.7.1.78 | D169A |
50% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, no reverse reaction |
652544 |
| 2.7.1.78 | D171A |
complete loss of activity |
676207 |
| 2.7.1.78 | D173A |
complete loss of activity |
676207 |
| 2.7.1.78 | D175A |
60% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, 68% of wild-type activity with CMP substrate |
652544 |
| 2.7.1.78 | D180A |
site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity |
642028 |
| 2.7.1.78 | D250A |
site-directed mutagenesis, increased activity compared to the wild-type , reduced 3'-phosphatase activity |
642028 |
| 2.7.1.78 | D254A |
inactive |
-, 761386 |
| 2.7.1.78 | D254A |
site-directed mutagenesis, increased activity compared to the wild-type, nearly no 3'-phosphatase activity remaining |
642028 |
| 2.7.1.78 | D278A |
site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining |
642028 |
