EC Number   |
Protein Variants |
Reference |
|---|
  2.7.1.181 | D350A |
88.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 95.2% with substrate D-mannose |
-, 728331 |
| 2.7.1.181 | D351A |
0.9% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.1% with substrate D-mannose |
728331 |
| 2.7.1.181 | D351E |
4.5% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.5% with substrate D-mannose |
728331 |
| 2.7.1.181 | DELTA398-418 |
6.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 4.7% with substrate D-mannose |
728331 |
| 2.7.1.181 | E274A |
45.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 66.6% with substrate D-mannose |
-, 728331 |
| 2.7.1.181 | H132A |
complete loss of activity |
-, 728331 |
| 2.7.1.181 | H133A |
complete loss of activity |
728331 |
| 2.7.1.181 | more |
uncoupling of WbdD kinase and methyltransferase activities, revealing that although the kinase activity is solely responsible for chain-length regulation, both activities are essential for CBM recognition and export |
-, 757222 |
| 2.7.1.181 | N34D/Q35E/H197E |
complete loss of activity |
728331 |
| 2.7.1.181 | R203A |
complete loss of activity |
-, 728331 |
